The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 65, 243-249, Copyright, 1937, by The Rockefeller Institute for Medical Research New York

ARTICLE

IMMUNOLOGICAL AND CHEMICAL INVESTIGATIONS OF VACCINE VIRUS : VI. ISOLATION OF A HEAT-STABLE, SEROLOGICALLY ACTIVE SUBSTANCE FROM TISSUES INFECTED WITH VACCINE VIRUS



Robert F. Parker M.D.1 and Thomas M. Rivers M.D.1

1 From the Hospital of The Rockefeller Institute for Medical Research

A method has been described by which a stable, serologically active substance has been isolated in a relatively pure state from tissues infected with vaccine virus. It has the characteristics of an alcohol-soluble protein which is not precipitated by boiling in a neutral aqueous solution. In a dilution of 1:640,000 it gives a precipitate when mixed with a serum containing antibodies against Craigie's S antigen of vaccine virus, but no visible reaction occurs when it is mixed with serum depleted of S antibodies by means of absorption.

Submitted on September 24, 1936


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