The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 61, 501-514, Copyright, 1935, by The Rockefeller Institute for Medical Research New York

ARTICLE

CULTIVATION OF THE VIRUS OF GRASSERIE IN SILKWORM TISSUE CULTURES

William Trager Ph.D.1

1 From the Department of Animal and Plant Pathology of The Rockefeller Institute for Medical Research, Princeton, N. J.

A medium has been developed in which certain cells from the gonads of female silkworms multiply and live for periods of 2 to 3 weeks.

In such tissue cultures, strains of silkworm grasserie virus were maintained in successive passages up to the number of ten. The virus multiplied greatly and typical polyhedral bodies formed in the cells of infected cultures.

 Submitted on January 3, 1935


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