The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 59, 519-528, Copyright, 1934, by The Rockefeller Institute for Medical Research New York

ARTICLE

QUANTITATIVE STUDIES ON THE PRECIPITIN REACTION : THE RÔLE OF MULTIPLE REACTIVE GROUPS IN ANTIGEN-ANTIBODY UNION AS ILLUSTRATED BY AN INSTANCE OF CROSS-PRECIPITATION



Michael Heidelberger Ph.D.1 and Forrest E. Kendall Ph.D.1

1 From the Department of Medicine, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York

1. Antisera to R-salt-azo-benzidine-azo-crystalline egg albumin give precipitates with crystalline egg albumin by virtue of their antidye content.

2. The quantitative course of the reactions with increasing amounts of antigen is very similar for the dye-antidye and egg albumin-anti-egg albumin systems, but differs markedly for the cross reaction between egg albumin and antidye.

3. A possible explanation for the occurrence of this one-sided crossreaction is given in terms of reactive groupings on the antigen and antibody.

4. A qualitative expression of the course of the cross-reaction is given in terms of the laws of classical chemistry.

 Submitted on January 8, 1934


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