Stoichiometry of the murine {gamma}{delta} T cell receptor

doi:10.1084/jem.20051886

Published online 17 January 2006 doi:10.1084/jem.20051886
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 203, Number 1, 47-52

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BRIEF DEFINITIVE REPORT

Stoichiometry of the murine ${gamma}$ ${delta}$ T cell receptor

Sandra M. Hayes and Paul E. Love

Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892

CORRESPONDENCE Sandra M. Hayes: hayessa{at}upstate.edu

The T cell receptor for antigen (TCR) complex is organized intotwo functional domains: the antigen-binding clonotypic heterodimerand the signal-transducing invariant CD3 and TCR ${zeta}$ chains. Inmost vertebrates, there are two different clonotypic heterodimers(TCR ${alpha}$ ß and TCR ${gamma}$ ${delta}$ ) that define the ${alpha}$ ß and ${gamma}$ ${delta}$ Tcell lineages, respectively. ${alpha}$ ß- and ${gamma}$ ${delta}$ TCRs also differin their invariant chain subunit composition, in that ${alpha}$ ßTCRscontain CD3 ${gamma}$ ${varepsilon}$ and CD3 ${delta}$ ${varepsilon}$ dimers, whereas ${gamma}$ ${delta}$ TCRs contain only CD3 ${gamma}$ ${varepsilon}$ dimers.This difference in subunit composition of the ${alpha}$ ß- and ${gamma}$ ${delta}$ TCRs raises the question of whether the stoichiometries of thesereceptor complexes are different. As the stoichiometry of themurine ${gamma}$ ${delta}$ TCR has not been previously investigated, we used twoquantitative immunofluorescent approaches to determine the valencyof TCR ${gamma}$ ${delta}$ heterodimers and CD3 ${gamma}$ ${varepsilon}$ dimers in surface murine ${gamma}$ ${delta}$ TCR complexes.Our results support a model of murine ${gamma}$ ${delta}$ TCR stoichiometry in whichthere are two CD3 ${gamma}$ ${varepsilon}$ dimers for every TCR ${gamma}$ ${delta}$ heterodimer.

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