The Journal of Experimental Medicine
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Published online 28 April 2003 doi:10.1084/jem.20030402
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© Rockefeller University Press, 0022-1007/2003/5/1165 $5.00
The Journal of Experimental Medicine, Volume 197, Number 9, 1165-1172

Expression of a Targeted {lambda}1 Light Chain Gene Is Developmentally Regulated and Independent of Ig{kappa} Rearrangements

Philipp Oberdoerffer1,2, Tatiana I. Novobrantseva1,2 and Klaus Rajewsky1,2

1 Center for Blood Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115
2 Institute for Genetics, University of Cologne, 121 Weyertal, 50932 Cologne, Germany

Address correspondence to K. Rajewsky, Center for Blood Research, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115. Phone: 617-278-3067; Fax: 617-278-3129; E-mail: rajewsky{at}cbr.med.harvard.edu

Immunoglobulin light chain (IgL) rearrangements occur more frequently at Ig{kappa} than at Ig{lambda}. Previous results suggested that the unrearranged Ig{kappa} locus negatively regulates Ig{lambda} transcription and/or rearrangement. Here, we demonstrate that expression of a VJ{lambda}1-joint inserted into its physiological position in the Ig{lambda} locus is independent of Ig{kappa} rearrangements. Expression of the inserted VJ{lambda}1 gene segment is developmentally controlled like that of a VJ{kappa}-joint inserted into the Ig{kappa} locus and furthermore coincides developmentally with the occurrence of Ig{kappa} rearrangements in wild-type mice. We conclude that developmentally controlled transcription of a gene rearrangement in the Ig{lambda} locus occurs in the presence of an unrearranged Ig{kappa} locus and is therefore not negatively regulated by the latter. Our data also indicate light chain editing in ~30% of {lambda}1 expressing B cell progenitors.

Key Words: gene rearrangement • B lymphocyte • light chain • receptor editing • development


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