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Brief Definitive Report |
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Repertoire by the Helix-Loop-Helix Proteins E2A and HEB in Nonlymphoid Cells
Correspondence to: Jimut Kanti Ghosh, Chattar Manzil Pl., Lucknow 226 001, India.
During specific stages of thymocyte development, the T cell receptor (TCR) locus is assembled from variable (V), diversity (D), and joining (J) gene segments. Proper TCR and V(D)J rearrangement during thymocyte development requires the presence of the E2A proteins. Here we show that E2A and a closely related protein, HEB, in the presence of recombination activating gene (RAG)1 and RAG2, each have the ability to activate TCR and rearrangement in human kidney cells. The coding joints are diverse, contain nucleotide deletions, and occasionally show the presence of P nucleotides. Interestingly, only a subset of V, D, and J segments are targeted by the E2A and HEB proteins. Thus, E2A and HEB permit localized accessibility of the TCR and loci to the recombination machinery. These data indicate that a distinct but diverse TCR repertoire can be induced in nonlymphoid cells by the mere presence of the V(D)J recombinase and the transcriptional regulators, E2A and HEB.
Key Words:
E2A, HEB, recombination activating gene, T cell receptor and rearrangements, chromatin accessibility
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