Distinct Cytokine Profiles of Neonatal Natural Killer T Cells after Expansion with Subsets of Dendritic Cells

doi:10.1084/jem.193.10.1221

Published online 21 May 2001.

This Article

	Full Text
	Full Text (PDF, 135K)
	PPT slides of all figures
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Kadowaki, N.
	Articles by Liu, Y.-J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Kadowaki, N.
	Articles by Liu, Y.-J.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0022-1007/2001/5/1221/ $5.00
The Journal of Experimental Medicine, Volume 193, Number 10, May 21, 2001 1221-1226

Brief Definitive Report

Distinct Cytokine Profiles of Neonatal Natural Killer T Cells after Expansion with Subsets of Dendritic Cells

Norimitsu Kadowaki^a, Svetlana Antonenko^a, Stephen Ho^a, Marie-Clotilde Rissoan^b, Vassili Soumelis^a, Steven A. Porcelli^c, Lewis L. Lanier^d, and Yong-Jun Liu^a
^a Department of Immunobiology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304
^b Schering-Plough Corporation, Laboratory for Immunological Research, 69571 Dardilly, France
^c Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461
^d Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143

Correspondence to: Yong-Jun Liu, DNAX Research Institute, 901 California Ave., Palo Alto, CA 94304. Tel:650-496-1157 Fax:650-496-1200 E-mail:yliu{at}dnax.org.

Natural killer T (NKT) cells are a highly conserved subset ofT cells that have been shown to play a critical role in suppressingT helper cell type 1–mediated autoimmune diseases andgraft versus host disease in an interleukin (IL)-4–dependentmanner. Thus, it is important to understand how the developmentof IL-4– versus interferon (IFN)- ${gamma}$ –producing NKTcells is regulated. Here, we show that NKT cells from adultblood and those from cord blood undergo massive expansion incell numbers (500–70,000-fold) during a 4-wk culture withIL-2, IL-7, phytohemagglutinin, anti-CD3, and anti-CD28 mAbs.Unlike adult NKT cells that preferentially produce both IL-4and IFN- ${gamma}$ , neonatal NKT cells preferentially produce IL-4 afterpolyclonal activation. Addition of type 2 dendritic cells (DC2)enhances the development of neonatal NKT cells into IL-4⁺IFN- ${gamma}$ ^-NKT2 cells, whereas addition of type 1 dendritic cells (DC1)induces polarization towards IL-4^-IFN- ${gamma}$ ⁺ NKT1 cells. Adult NKTcells display limited plasticity for polarization induced byDC1 or DC2. Thus, newly generated NKT cells may possess thepotent ability to develop into IL-4⁺IFN- ${gamma}$ ^- NKT2 cells in responseto appropriate stimuli and may thereafter acquire the tendencyto produce both IL-4 and IFN- ${gamma}$ .

Key Words: cord blood, interleukin 4, interferon ${gamma}$ , autoimmune diseases,graft versus host disease

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Borderia, A. V., Hartmann, B. M., Fernandez-Sesma, A., Moran, T. M., Sealfon, S. C. (2008). Antiviral-Activated Dendritic Cells: A Paracrine-Induced Response State. J. Immunol. 181: 6872-6881 [Abstract] [Full Text]
Baev, D. V., Caielli, S., Ronchi, F., Coccia, M., Facciotti, F., Nichols, K. E., Falcone, M. (2008). Impaired SLAM-SLAM Homotypic Interaction between Invariant NKT Cells and Dendritic Cells Affects Differentiation of IL-4/IL-10-Secreting NKT2 Cells in Nonobese Diabetic Mice. J. Immunol. 181: 869-877 [Abstract] [Full Text]
Yamaura, A., Hotta, C., Nakazawa, M., Van Kaer, L., Minami, M. (2008). Human invariant V{alpha}24+ natural killer T cells acquire regulatory functions by interacting with IL-10-treated dendritic cells. Blood 111: 4254-4263 [Abstract] [Full Text]
Shen, W., Stone, K., Jales, A., Leitenberg, D., Ladisch, S. (2008). Inhibition of TLR Activation and Up-Regulation of IL-1R-Associated Kinase-M Expression by Exogenous Gangliosides. J. Immunol. 180: 4425-4432 [Abstract] [Full Text]
Wang, Y.-H., Angkasekwinai, P., Lu, N., Voo, K. S., Arima, K., Hanabuchi, S., Hippe, A., Corrigan, C. J., Dong, C., Homey, B., Yao, Z., Ying, S., Huston, D. P., Liu, Y.-J. (2007). IL-25 augments type 2 immune responses by enhancing the expansion and functions of TSLP-DC activated Th2 memory cells. JEM 204: 1837-1847 [Abstract] [Full Text]
Jung, I. D., Lee, J. S., Kim, Y. J., Jeong, Y.-I., Lee, C.-M., Baumruker, T., Billlich, A., Banno, Y., Lee, M. G., Ahn, S.-C., Park, W. S., Han, J., Park, Y.-M. (2007). Sphingosine kinase inhibitor suppresses a Th1 polarization via the inhibition of immunostimulatory activity in murine bone marrow-derived dendritic cells. Int Immunol 19: 411-426 [Abstract] [Full Text]
Coquet, J. M., Kyparissoudis, K., Pellicci, D. G., Besra, G., Berzins, S. P., Smyth, M. J., Godfrey, D. I. (2007). IL-21 Is Produced by NKT Cells and Modulates NKT Cell Activation and Cytokine Production. J. Immunol. 178: 2827-2834 [Abstract] [Full Text]
Schott, M. (2006). Immunesurveillance by dendritic cells: potential implication for immunotherapy of endocrine cancers.. Endocr Relat Cancer 13: 779-795 [Abstract] [Full Text]
Montoya, C. J., Jie, H.-B., Al-Harthi, L., Mulder, C., Patino, P. J., Rugeles, M. T., Krieg, A. M., Landay, A. L., Wilson, S. B. (2006). Activation of Plasmacytoid Dendritic Cells with TLR9 Agonists Initiates Invariant NKT Cell-Mediated Cross-Talk with Myeloid Dendritic Cells. J. Immunol. 177: 1028-1039 [Abstract] [Full Text]
Kotsianidis, I., Silk, J. D., Spanoudakis, E., Patterson, S., Almeida, A., Schmidt, R. R., Tsatalas, C., Bourikas, G., Cerundolo, V., Roberts, I. A. G., Karadimitris, A. (2006). Regulation of hematopoiesis in vitro and in vivo by invariant NKT cells. Blood 107: 3138-3144 [Abstract] [Full Text]
Sen, Y., Yongyi, B., Yuling, H., Luokun, X., Li, H., Jie, X., Tao, D., Gang, Z., Junyan, L., Chunsong, H., Zhang, X., Youxin, J., Feili, G., Boquan, J., Jinquan, T. (2005). V{alpha}24-Invariant NKT Cells from Patients with Allergic Asthma Express CCR9 at High Frequency and Induce Th2 Bias of CD3+ T Cells upon CD226 Engagement. J. Immunol. 175: 4914-4926 [Abstract] [Full Text]
Rossi, M., Young, J. W. (2005). Human Dendritic Cells: Potent Antigen-Presenting Cells at the Crossroads of Innate and Adaptive Immunity. J. Immunol. 175: 1373-1381 [Abstract] [Full Text]
Tabata, S., Kadowaki, N., Kitawaki, T., Shimaoka, T., Yonehara, S., Yoshie, O., Uchiyama, T. (2005). Distribution and kinetics of SR-PSOX/CXCL16 and CXCR6 expression on human dendritic cell subsets and CD4+ T cells. J. Leukoc. Biol. 77: 777-786 [Abstract] [Full Text]
Fugier-Vivier, I. J., Rezzoug, F., Huang, Y., Graul-Layman, A. J., Schanie, C. L., Xu, H., Chilton, P. M., Ildstad, S. T. (2005). Plasmacytoid precursor dendritic cells facilitate allogeneic hematopoietic stem cell engraftment. JEM 201: 373-383 [Abstract] [Full Text]
Gao, J.-X., Liu, X., Wen, J., Caligiuri, M. A., Stroynowski, I., Zheng, P., Liu, Y. (2003). Two-signal requirement for activation and effector function of natural killer cell response to allogeneic tumor cells. Blood 102: 4456-4463 [Abstract] [Full Text]
Yang, Y., Ueno, A., Bao, M., Wang, Z., Im, J. S., Porcelli, S., Yoon, J.-W. (2003). Control of NKT Cell Differentiation by Tissue-Specific Microenvironments. J. Immunol. 171: 5913-5920 [Abstract] [Full Text]
Gansuvd, B., Hubbard, W. J., Hutchings, A., Thomas, F. T., Goodwin, J., Wilson, S. B., Exley, M. A., Thomas, J. M. (2003). Phenotypic and Functional Characterization of Long-Term Cultured Rhesus Macaque Spleen-Derived NKT Cells. J. Immunol. 171: 2904-2911 [Abstract] [Full Text]
Jefford, M., Schnurr, M., Toy, T., Masterman, K.-A., Shin, A., Beecroft, T., Tai, T. Y., Shortman, K., Shackleton, M., Davis, I. D., Parente, P., Luft, T., Chen, W., Cebon, J., Maraskovsky, E. (2003). Functional comparison of DCs generated in vivo with Flt3 ligand or in vitro from blood monocytes: differential regulation of function by specific classes of physiologic stimuli. Blood 102: 1753-1763 [Abstract] [Full Text]
Gillessen, S., Naumov, Y. N., Nieuwenhuis, E. E. S., Exley, M. A., Lee, F. S., Mach, N., Luster, A. D., Blumberg, R. S., Taniguchi, M., Balk, S. P., Strominger, J. L., Dranoff, G., Wilson, S. B. (2003). CD1d-restricted T cells regulate dendritic cell function and antitumor immunity in a granulocyte-macrophage colony-stimulating factor-dependent fashion. Proc. Natl. Acad. Sci. USA 100: 8874-8879 [Abstract] [Full Text]
van der Vliet, H. J. J., Molling, J. W., Nishi, N., Masterson, A. J., Kolgen, W., Porcelli, S. A., van den Eertwegh, A. J. M., von Blomberg, B. M. E., Pinedo, H. M., Giaccone, G., Scheper, R. J. (2003). Polarization of V{alpha}24+ V{beta}11+ Natural Killer T Cells of Healthy Volunteers and Cancer Patients Using {alpha}-Galactosylceramide-loaded and Environmentally Instructed Dendritic Cells. Cancer Res. 63: 4101-4106 [Abstract] [Full Text]
Matsuda, J. L., Gapin, L., Baron, J. L., Sidobre, S., Stetson, D. B., Mohrs, M., Locksley, R. M., Kronenberg, M. (2003). Mouse V{alpha}14i natural killer T cells are resistant to cytokine polarization in vivo. Proc. Natl. Acad. Sci. USA 100: 8395-8400 [Abstract] [Full Text]
Kang, S.-M., Compans, R. W. (2003). Enhancement of Mucosal Immunization with Virus-Like Particles of Simian Immunodeficiency Virus. J. Virol. 77: 3615-3623 [Abstract] [Full Text]
Araki, M., Kondo, T., Gumperz, J. E., Brenner, M. B., Miyake, S., Yamamura, T. (2003). Th2 bias of CD4+ NKT cells derived from multiple sclerosis in remission. Int Immunol 15: 279-288 [Abstract] [Full Text]
Schotte, R., Rissoan, M.-C., Bendriss-Vermare, N., Bridon, J.-M., Duhen, T., Weijer, K., Briere, F., Spits, H. (2003). The transcription factor Spi-B is expressed in plasmacytoid DC precursors and inhibits T-, B-, and NK-cell development. Blood 101: 1015-1023 [Abstract] [Full Text]
Gurney, K. B., Yang, O. O., Wilson, S. B., Uittenbogaart, C. H. (2002). TCR{gamma}{delta}+ and CD161+ Thymocytes Express HIV-1 in the SCID-hu Mouse, Potentially Contributing to Immune Dysfunction in HIV Infection. J. Immunol. 169: 5338-5346 [Abstract] [Full Text]
Gadue, P., Stein, P. L. (2002). NK T Cell Precursors Exhibit Differential Cytokine Regulation and Require Itk for Efficient Maturation. J. Immunol. 169: 2397-2406 [Abstract] [Full Text]
Gorgun, G., Miller, K. B., Foss, F. M. (2002). Immunologic mechanisms of extracorporeal photochemotherapy in chronic graft-versus-host disease. Blood 100: 941-947 [Abstract] [Full Text]
Sandberg, J. K., Fast, N. M., Palacios, E. H., Fennelly, G., Dobroszycki, J., Palumbo, P., Wiznia, A., Grant, R. M., Bhardwaj, N., Rosenberg, M. G., Nixon, D. F. (2002). Selective Loss of Innate CD4+ V{alpha}24 Natural Killer T Cells in Human Immunodeficiency Virus Infection. J. Virol. 76: 7528-7534 [Abstract] [Full Text]
Kim, C. H., Johnston, B., Butcher, E. C. (2002). Trafficking machinery of NKT cells: shared and differential chemokine receptor expression among Valpha 24+Vbeta 11+ NKT cell subsets with distinct cytokine-producing capacity. Blood 100: 11-16 [Abstract] [Full Text]
Trobonjaca, Z., Kroger, A., Stober, D., Leithauser, F., Moller, P., Hauser, H., Schirmbeck, R., Reimann, J. (2002). Activating Immunity in the Liver. II. IFN-{beta} Attenuates NK Cell-Dependent Liver Injury Triggered by Liver NKT Cell Activation. J. Immunol. 168: 3763-3770 [Abstract] [Full Text]
Laloux, V., Beaudoin, L., Ronet, C., Lehuen, A. (2002). Phenotypic and Functional Differences Between NKT Cells Colonizing Splanchnic and Peripheral Lymph Nodes. J. Immunol. 168: 3251-3258 [Abstract] [Full Text]
Mellor, E. A., Austen, K. F., Boyce, J. A. (2002). Cysteinyl Leukotrienes and Uridine Diphosphate Induce Cytokine Generation by Human Mast Cells Through an Interleukin 4-regulated Pathway that Is Inhibited by Leukotriene Receptor Antagonists. JEM 195: 583-592 [Abstract] [Full Text]