The Journal of Experimental Medicine
PBL InterferonSource
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 18 December 2000.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 236K)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Blom, B.
Right arrow Articles by Liu, Y.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blom, B.
Right arrow Articles by Liu, Y.-J.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Medline Plus Health Information
*Stem Cells
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
© The Rockefeller University Press, 0022-1007/2000/12/1785/ $5.00
The Journal of Experimental Medicine, Volume 192, Number 12, December 18, 2000 1785-1796

Original Article

Generation of Interferon {alpha}–producing Predendritic Cell (Pre-DC)2 from Human CD34+ Hematopoietic Stem Cells

Bianca Bloma, Stephen Hoa, Svetlana Antonenkoa, and Yong-Jun Liua
a Department of Immunobiology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304

Correspondence to: Yong-Jun Liu, Department of Immunobiology, DNAX Research Institute of Molecular and Cellular Biology, 901 California Ave., Palo Alto, CA 94304. Tel:650-496-1157 Fax:650-496-1200 E-mail:yliu{at}dnax.org.

Upon viral stimulation, the natural interferon (IFN)-{alpha}/ß–producing cells (IPCs; also known as pre-dendritic cells (DCs (2)) in human blood and peripheral lymphoid tissues rapidly produce huge amounts of IFN-{alpha}/ß. After performing this innate antiviral immune response, IPCs can differentiate into DCs and strongly stimulate T cell–mediated adaptive immune responses. Using four-color immunofluorescence flow cytometry, we have mapped the developmental pathway of pre-DC2/IPCs from CD34+ hematopoietic stem cells in human fetal liver, bone marrow, and cord blood. At least four developmental stages were identified, including CD34++CD45RA- early progenitor cells, CD34++CD45RA+ late progenitor cells, CD34+CD45RA++CD4+interleukin (IL)-3R{alpha}++ pro-DC2, and CD34-CD45RA++ CD4+IL-3R{alpha}++ pre-DC2/IPCs. Pro-DC2s have already acquired the capacity to produce large amounts of IFN-{alpha}/ß upon viral stimulation and to differentiate into DCs in culture with IL-3 and CD40 ligand. CD34++CD45RA- early progenitor cells did not have the capacity to produce large amounts of IFN-{alpha}/ß in response to viral stimulation; however, they can be induced to undergo proliferation and differentiation into IPCs/pre-DC2 in culture with FLT3 ligand.

Key Words: dendritic cells, interferon {alpha}/ß–producing cells, stem cells, FLT3 ligand, interferon-producing cell


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS