Brief Definitive Report

A Processed Pseudogene Codes for a New Antigen Recognized by a CD8⁺ T Cell Clone on Melanoma

Correspondence to: Richard Breathnach, INSERM U463, 9 Quai Moncousu, 44093 Nantes cedex 01, France. Tel:33-02-40-08-47-50 Fax:33-02-40-36-66-97 E-mail:breathna{at}nantes.inserm.fr.

The M88.7 T cell clone recognizes an antigen presented by HLA B*1302 on the melanoma cell line M88. A cDNA encoding this antigen (NA88-A) was isolated using a library transfection approach. Analysis of the genomic gene's sequence identified it is a processed pseudogene, derived from a retrotranscript of mRNA coding for homeoprotein HPX42B. The NA88-A gene exhibits several premature stop codons, deletions, and insertions relative to the HPX42B gene. In NA88-A RNA, a short open reading frame codes for the peptide MTQGQHFLQKV from which antigenic peptides are derived; a stop codon follows the peptide's COOH-terminal Val codon. Part of the HPX42B mRNA's 3' untranslated region codes for a peptide of similar sequence (MTQGQHFSQKV). If produced, this peptide can be recognized by M88.7 T cells. However, in HPX42B mRNA, the peptide's COOH-terminal Val codon is followed by a Trp codon. As a result, expression of HPX42B mRNA does not lead to antigen production. A model is proposed for events that participated in creation of a gene coding for a melanoma antigen from a pseudogene.

Key Words: peptide, epitope, processing, tumor immunity, CTL

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Godet, Y., Moreau-Aubry, A., Guilloux, Y., Vignard, V., Khammari, A., Dreno, B., Jotereau, F., Labarriere, N. (2008). MELOE-1 is a new antigen overexpressed in melanomas and involved in adoptive T cell transfer efficiency. JEM 205: 2673-2682 [Abstract] [Full Text]  
• Geffroy-Luseau, A., Moreau-Aubry, A., Bataille, R., Pellat-Deceunynck, C. (2005). Allogeneic T lymphocytes as a source of peptide-dependent T cells specific for myeloma cells. Int Immunol 17: 1193-1200 [Abstract] [Full Text]  
• Godefroy, E., Moreau-Aubry, A., Diez, E., Dreno, B., Jotereau, F., Guilloux, Y. (2005). {alpha}v{beta}3-dependent cross-presentation of matrix metalloproteinase-2 by melanoma cells gives rise to a new tumor antigen. JEM 202: 61-72 [Abstract] [Full Text]  
• Frints, S. G. M., Marynen, P., Hartmann, D., Fryns, J.-P., Steyaert, J., Schachner, M., Rolf, B., Craessaerts, K., Snellinx, A., Hollanders, K., D'Hooge, R., De Deyn, P. P., Froyen, G. (2003). CALL interrupted in a patient with non-specific mental retardation: gene dosage-dependent alteration of murine brain development and behavior. Hum Mol Genet 12: 1463-1474 [Abstract] [Full Text]  
• Espinoza-Delgado, I. (2002). Cancer Vaccines. The Oncologist 7: 20-33 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS