The Journal of Experimental Medicine
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© The Rockefeller University Press, 0022-1007/2000/2/669/ $5.00
The Journal of Experimental Medicine, Volume 191, Number 4, February 21, 2000 669-682

Original Article

Fc{gamma} Receptor–mediated Phagocytosis in Macrophages Lacking the Src Family Tyrosine Kinases Hck, Fgr, and Lyn

Cheryl J. Fitzer-Attasa,b, Malcolm Lowrya,b, Mary T. Crowleya,b, Alexander J. Finna,b, Fanying Mengc, Anthony L. DeFrancoa,b, and Clifford A. Lowellc
a From the Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143
b From the George Williams Hooper Foundation, University of California at San Francisco, San Francisco, California 94143
c From the Department of Laboratory Medicine, University of California at San Francisco, San Francisco, California 94143

Correspondence to: Clifford A. Lowell, Department of Laboratory Medicine, University of California at San Francisco, 513 Parnassus Ave., San Francisco, CA 94143. Tel:415-476-2963 Fax:415-502-6497 E-mail:clowell{at}cgl.ucsf.edu.

Macrophage Fc{gamma} receptors (Fc{gamma}Rs) mediate the uptake and destruction of antibody-coated viruses, bacteria, and parasites. We examined Fc{gamma}R signaling and phagocytic function in bone marrow–derived macrophages from mutant mice lacking the major Src family kinases expressed in these cells, Hck, Fgr, and Lyn. Many Fc{gamma}R-induced functional responses and signaling events were diminished or delayed in these macrophages, including immunoglobulin (Ig)G-coated erythrocyte phagocytosis, respiratory burst, actin cup formation, and activation of Syk, phosphatidylinositol 3-kinase, and extracellular signal–regulated kinases 1 and 2. Significant reduction of IgG-dependent phagocytosis was not seen in hck-/-fgr-/- or lyn-/- cells, although the single mutant lyn-/- macrophages did manifest signaling defects. Thus, Src family kinases clearly have roles in two events leading to Fc{gamma}R-mediated phagocytosis, one involving initiation of actin polymerization and the second involving activation of Syk and subsequent internalization. Since Fc{gamma}R-mediated phagocytosis did occur at modest levels in a delayed fashion in triple mutant macrophages, these Src family kinases are not absolutely required for uptake of IgG-opsonized particles.

Key Words: actin polymerization, Fc{gamma} receptors, macrophage, phagocytosis, Src family kinases


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