The Journal of Experimental Medicine
Track the topics, authors and articles important to you
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 6 June 1999.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 310K)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ruetsch, N. R.
Right arrow Articles by Bosma, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ruetsch, N. R.
Right arrow Articles by Bosma, M. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
© The Rockefeller University Press, 0022-1007/2000/6/1933/ $5.00
The Journal of Experimental Medicine, Volume 191, Number 11, June 5, 2000 1933-1944

Original Article

Unexpected Rearrangement and Expression of the Immunoglobulin {lambda}1 Locus in Scid Mice

Norman R. Ruetscha, Gayle C. Bosmaa, and Melvin J. Bosmaa
a Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, Pennsylvania 19111

Correspondence to: Melvin J. Bosma, Fox Chase Cancer Center, Institute for Cancer Research, 7701 Burholme Ave., Philadelphia, PA 19111. Tel:215-728-3630 Fax:215-728-2412 E-mail:MJ_Bosma{at}fccc.edu.

In severe combined immunodeficient (scid) mice, V(D)J recombination is severely impaired due to a recessive mutation (scid). Thus, we were surprised to find in this study that V{lambda}1–J{lambda}1 rearrangement is routinely detectable in scid fetal liver, adult bone marrow, and spleen in the apparent absence of completed VH–DJH and V{kappa}–J{kappa} rearrangements. Particularly surprising, we found the level of V{lambda}1–J{lambda}1 rearrangement in scid fetal liver to be comparable to that in fetal liver of wild-type mice. The majority of scid V{lambda}1–J{lambda}1 rearrangements contained abnormal deletions at the VJ junction, consistent with the known effect of scid. However, ~15% of V{lambda}1–J{lambda}1 rearrangements lacked abnormal deletions. Productive {lambda}1 transcripts resulting from in-frame rearrangements were readily detectable in scid adult bone marrow and spleen, consistent with our ability to detect {lambda}1-expressing cells by flow cytometry in the spleens of bcl-2–transgenic scid mice. Strikingly, {lambda}1 transcripts from individual scid mice often showed VJ junctional sequences with the same recurring palindromic (P) additions of three, four, or five nucleotides. To account for these findings, we suggest that (a) nonhomologous end joining of V{lambda}1 and J{lambda}1 coding ends in fetal B lineage cells may not be (severely) impaired by scid; (b) recurring P additions in scid {lambda}1 transcripts may reflect certain molecular constraints imposed by scid on the resolution of V{lambda}1 and J{lambda}1 hairpin coding ends; and (c), scid lymphocytes with productively rearranged V{lambda}1 and J{lambda}1 elements may differentiate into recombinase-inactive cells and emigrate from bone marrow to spleen.

Key Words: B cell differentiation, pro-B cells, premature Ig{lambda} recombination, VJ{lambda} junctional diversity, P additions


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS