The Journal of Experimental Medicine
Track the topics, authors and articles important to you
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 156K)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Naidenko, O. V.
Right arrow Articles by Kronenberg, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Naidenko, O. V.
Right arrow Articles by Kronenberg, M.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene*UniGene
*Compound via MeSH
*Substance via MeSH
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
© The Rockefeller University Press, 0022-1007/1999/10/1069/ $5.00
The Journal of Experimental Medicine, Volume 190, Number 8, October 18, 1999 1069-1080

Binding and Antigen Presentation of Ceramide-containing Glycolipids by Soluble Mouse and Human CD1d Molecules

Olga V. Naidenkoa,e, Juli K. Mahera,e, William A. Ernsta,b,c, Teruyuki Sakaif, Robert L. Modlina,c, and Mitchell Kronenberga,d,e
a Department of Microbiology and Immunology and the Molecular Biology Institute, Department of Medicine, University of California at Los Angeles, Los Angeles, California 90095
b Department of Microbiology and Molecular Genetics, Department of Medicine, University of California at Los Angeles, Los Angeles, California 90095
c Division of Dermatology, Department of Medicine, University of California at Los Angeles, Los Angeles, California 90095
d Division of Digestive Diseases, Department of Medicine, University of California at Los Angeles, Los Angeles, California 90095
e La Jolla Institute for Allergy and Immunology, San Diego, California 92121
f Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd., Gunma 370-12, Japan

Correspondence to: Mitchell Kronenberg, La Jolla Institute for Allergy and Immunology, 10355 Science Center Dr., San Diego, CA 92121. Tel:858-678-4540 Fax:858-678-4595 E-mail:mitch{at}liai.org.

We have purified soluble mouse and human CD1d molecules to assess the structural requirements for lipid antigen presentation by CD1. Plate-bound CD1d molecules from either species can present the glycolipid {alpha}-galactosyl ceramide ({alpha}-GalCer) to mouse natural killer T cells, formally demonstrating both the in vitro formation of antigenic complexes, and the presentation of {alpha}-GalCer by these two CD1d molecules. Using surface plasmon resonance, we show that at neutral pH, mouse CD1 and human CD1d bind to immobilized {alpha}-GalCer, unlike human CD1b, which requires acidic pH for lipid antigen binding. The CD1d molecules can also bind both to the nonantigenic ß-GalCer and to phosphatidylethanolamine, indicating that diverse lipids can bind to CD1d. These studies provide the first quantitative analysis of monomeric lipid antigen–CD1 interactions, and they demonstrate that the orientation of the galactose, or even the nature of the polar head group, are likely to be more important for T cell receptor contact than CD1d binding.

Key Words: antigen presentation, CD1, glycolipid, binding assay


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS