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/ Mice
Overtly React with Self-Major Histocompatibility Complex
Molecules upon Restoration of Normal Surface Density of
T Cell Receptor-CD3 Complex
By
From the Centre d'Immunologie Institut National de la Santè et de la Recherche Mèdicale-Centre
National de Recherche Scientifique de Marseille-Luminy, 13288 Marseille Cedex 9, France
CD3-
/
-deficient mice have small thymuses containing cells that show a profound reduction
in the surface levels of T cell receptors and terminate their differentiation at the CD4+CD8+
stage. Rather unexpectedly, CD3
or very low single positive T cells accumulate over time in the
spleen and lymph nodes of CD3-/-deficient mice after a process dependent on MHC expression. Fusion of these peripheral T cells with a CD3--positive derivative of the BW5147
TCR-
/
thymoma resulted in hybridomas that do express an heterogeneous set of T cell
receptor / dimers at their surface and at density comparable to those found in hybridomas
derived from wild-type peripheral T cells. We have investigated the specificities of these T cell
receptors using spleen cells from congenic and mutant mouse strains, and showed that the majority of them readily recognized self-MHC class I or class II molecules. These results demonstrate that by increasing the density and/or output of the T cell receptors expressed in peripheral T cells, one can confer them with the capacity to respond to normal density of self-MHC
molecules.
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