Journal of Experimental Medicine, Vol 182, 163-174, Copyright © 1995 by Rockefeller University Press

ARTICLES

Antigen capture and major histocompatibility class II compartments of freshly isolated and cultured human blood dendritic cells

HW Nijman, MJ Kleijmeer, MA Ossevoort, VM Oorschot, MP Vierboom, M van de Keur, P Kenemans, WM Kast, HJ Geuze and CJ Melief
Department of Immunohematology and Blood Bank, Academic Hospital Leiden, The Netherlands.

Dendritic cells (DC) represent potent antigen-presenting cells for the induction of T cell-dependent immune responses. Previous work on antigen uptake and presentation by human DC is based largely on studies of blood DC that have been cultured for various periods of time before analysis. These cultured cells may therefore have undergone a maturation process from precursors that have different capacities for antigen capture and presentation. We have now used immunoelectron microscopy and antigen presentation assays to compare freshly isolated DC (f-DC) and cultured DC (c-DC). f-DC display a round appearance, whereas c-DC display characteristic long processes. c-DC express much more cell surface major histocompatibility complex (MHC) class II than f-DC. The uptake of colloidal gold-labeled bovine serum albumin (BSA), however, is greater in f-DC, as is the presentation of 65-kD heat shock protein to T cell clones. The most striking discovery is that the majority of MHC class II molecules in both f-DC and c-DC occur in intracellular vacuoles with a complex shape (multivesicular and multilaminar). These MHC class II enriched compartments (MIIC) represent the site to which BSA is transported within 30 min. Although MIIC appear as more dense structures with less MHC class II molecules in f-DC than c-DC, the marker characteristics are very similar. The MIIC in both types of DC are acidic, contain invariant chain, and express the recently described HLA-DM molecule that can contribute to antigen presentation. CD19+ peripheral blood B cells have fewer MIIC and surface MHC class II expression than DCs, while monocytes had low levels of MIIC and surface MHC class II. These results demonstrate in dendritic cells the elaborate development of MIIC expressing several of the components that are required for efficient antigen presentation.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Spadaro, M., Caorsi, C., Ceruti, P., Varadhachary, A., Forni, G., Pericle, F., Giovarelli, M. (2008). Lactoferrin, a major defense protein of innate immunity, is a novel maturation factor for human dendritic cells. FASEB J. 22: 2747-2757 [Abstract] [Full Text]  
• Verkade, M. A., van Druningen, C. J., Vaessen, L. M. B., Hesselink, D. A., Weimar, W., Betjes, M. G. H. (2007). Functional impairment of monocyte-derived dendritic cells in patients with severe chronic kidney disease. Nephrol Dial Transplant 22: 128-138 [Abstract] [Full Text]  
• Cappello, P., Fraone, T., Barberis, L., Costa, C., Hirsch, E., Elia, A. R., Caorsi, C., Musso, T., Novelli, F., Giovarelli, M. (2006). CC-Chemokine Ligand 16 Induces a Novel Maturation Program in Human Immature Monocyte-Derived Dendritic Cells. J. Immunol. 177: 6143-6151 [Abstract] [Full Text]  
• Potolicchio, I., Chitta, S., Xu, X., Fonseca, D., Crisi, G., Horejsi, V., Strominger, J. L., Stern, L. J., Raposo, G., Santambrogio, L. (2005). Conformational Variation of Surface Class II MHC Proteins during Myeloid Dendritic Cell Differentiation Accompanies Structural Changes in Lysosomal MIIC. J. Immunol. 175: 4935-4947 [Abstract] [Full Text]  
• Moghaddami, M., Mayrhofer, G., Cleland, L. G. (2005). MHC class II compartment, endocytosis and phagocytic activity of macrophages and putative dendritic cells isolated from normal tissues rich in synovium. Int Immunol 17: 1117-1130 [Abstract] [Full Text]  
• Morelli, A. E., Larregina, A. T., Shufesky, W. J., Sullivan, M. L. G., Stolz, D. B., Papworth, G. D., Zahorchak, A. F., Logar, A. J., Wang, Z., Watkins, S. C., Falo, L. D. Jr, Thomson, A. W. (2004). Endocytosis, intracellular sorting, and processing of exosomes by dendritic cells. Blood 104: 3257-3266 [Abstract] [Full Text]  
• Moldenhauer, A., Frank, R. C., Pinilla-Ibarz, J., Holland, G., Boccuni, P., Scheinberg, D. A., Salama, A., Seeger, K., Moore, M. A. S., Nimer, S. D. (2004). Histone deacetylase inhibition improves dendritic cell differentiation of leukemic blasts with AML1-containing fusion proteins. J. Leukoc. Biol. 76: 623-633 [Abstract] [Full Text]  
• Takahashi, M., Kurosaka, K., Kobayashi, Y. (2004). Immature dendritic cells reduce proinflammatory cytokine production by a coculture of macrophages and apoptotic cells in a cell-to-cell contact-dependent manner. J. Leukoc. Biol. 75: 865-873 [Abstract] [Full Text]  
• Boes, M., Bertho, N., Cerny, J., Op den Brouw, M., Kirchhausen, T., Ploegh, H. (2003). T Cells Induce Extended Class II MHC Compartments in Dendritic Cells in a Toll-Like Receptor-Dependent Manner. J. Immunol. 171: 4081-4088 [Abstract] [Full Text]  
• Setterblad, N., Roucard, C., Bocaccio, C., Abastado, J.-P., Charron, D., Mooney, N. (2003). Composition of MHC class II-enriched lipid microdomains is modified during maturation of primary dendritic cells. J. Leukoc. Biol. 74: 40-48 [Abstract] [Full Text]  
• Bykovskaia, S. N., Shurin, G. V., Graner, S., Bunker, M. L., Olson, W., Thomas, R., Shurin, M. R., Marks, S., Storkus, W. J., Shogan, J. (2002). Differentiation of Immunostimulatory Stem-Cell- and Monocyte-Derived Dendritic Cells Involves Maturation of Intracellular Compartments Responsible for Antigen Presentation and Secretion. Stem Cells 20: 380-393 [Abstract] [Full Text]  
• Kleijmeer, M., Ramm, G., Schuurhuis, D., Griffith, J., Rescigno, M., Ricciardi-Castagnoli, P., Rudensky, A. Y., Ossendorp, F., Melief, C. J.M., Stoorvogel, W., Geuze, H. J. (2001). Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. JCB 155: 53-64 [Abstract] [Full Text]  
• Hamerman, J. A., Aderem, A. (2001). Functional Transitions in Macrophages During In Vivo Infection with Mycobacterium bovis Bacillus Calmette-Guerin. J. Immunol. 167: 2227-2233 [Abstract] [Full Text]  
• Ruedl, C., Koebel, P., Karjalainen, K. (2001). In Vivo-Matured Langerhans Cells Continue to Take Up and Process Native Proteins Unlike In Vitro-Matured Counterparts. J. Immunol. 166: 7178-7182 [Abstract] [Full Text]  
• Auffermann-Gretzinger, S., Keeffe, E. B., Levy, S. (2001). Impaired dendritic cell maturation in patients with chronic, but not resolved, hepatitis C virus infection. Blood 97: 3171-3176 [Abstract] [Full Text]  
• Engering, A., Pieters, J. (2001). Association of distinct tetraspanins with MHC class II molecules at different subcellular locations in human immature dendritic cells. Int Immunol 13: 127-134 [Abstract] [Full Text]  
• Baron, C, Raposo, G, Scholl, S., Bausinger, H, Tenza, D, Bohbot, A, Pouillart, P, Goud, B, Hanau, D, Salamero, J (2001). Modulation of MHC class II transport and lysosome distribution by macrophage-colony stimulating factor in human dendritic cells derived from monocytes. J. Cell Sci. 114: 999-1010 [Abstract]  
• Askew, D., Chu, R. S., Krieg, A. M., Harding, C. V. (2000). CpG DNA Induces Maturation of Dendritic Cells with Distinct Effects on Nascent and Recycling MHC-II Antigen-Processing Mechanisms. J. Immunol. 165: 6889-6895 [Abstract] [Full Text]  
• Dzionek, A., Fuchs, A., Schmidt, P., Cremer, S., Zysk, M., Miltenyi, S., Buck, D. W., Schmitz, J. (2000). BDCA-2, BDCA-3, and BDCA-4: Three Markers for Distinct Subsets of Dendritic Cells in Human Peripheral Blood. J. Immunol. 165: 6037-6046 [Abstract] [Full Text]  
• Kato, M., Neil, T. K., Fearnley, D. B., McLellan, A. D., Vuckovic, S., Hart, D. N. J. (2000). Expression of multilectin receptors and comparative FITC-dextran uptake by human dendritic cells. Int Immunol 12: 1511-1519 [Abstract] [Full Text]  
• Garcia-Del Portillo, F., Jungnitz, H., Rohde, M., Guzman, C. A. (2000). Interaction of Salmonella enterica Serotype Typhimurium with Dendritic Cells Is Defined by Targeting to Compartments Lacking Lysosomal Membrane Glycoproteins. Infect. Immun. 68: 2985-2991 [Abstract] [Full Text]  
• Inaba, K., Turley, S., Iyoda, T., Yamaide, F., Shimoyama, S., e Sousa, C. R., Germain, R. N., Mellman, I., Steinman, R. M. (2000). The Formation of Immunogenic Major Histocompatibility Complex Class II-Peptide Ligands in Lysosomal Compartments of Dendritic Cells Is Regulated by Inflammatory Stimuli. JEM 191: 927-936 [Abstract] [Full Text]  
• Steinman, R. M., Turley, S., Mellman, I., Inaba, K. (2000). The Induction of Tolerance by Dendritic Cells That Have Captured Apoptotic Cells. JEM 191: 411-416 [Full Text]  
• Denzer, K, Kleijmeer, M., Heijnen, H., Stoorvogel, W, Geuze, H. (2000). Exosome: from internal vesicle of the multivesicular body to intercellular signaling device. J. Cell Sci. 113: 3365-3374 [Abstract]  
• Ashany, D., Savir, A., Bhardwaj, N., Elkon, K. B. (1999). Dendritic Cells Are Resistant to Apoptosis Through the Fas (CD95/APO-1) Pathway. J. Immunol. 163: 5303-5311 [Abstract] [Full Text]  
• Fanger, N. A., Maliszewski, C. R., Schooley, K., Griffith, T. S. (1999). Human Dendritic Cells Mediate Cellular Apoptosis via Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL). JEM 190: 1155-1164 [Abstract] [Full Text]  
• Davidson, H. W. (1999). Direct Transport of Newly Synthesized HLA-DR from the trans-Golgi Network to Major Histocompatibility Complex Class II Containing Compartments (MIICS) Demonstrated Using a Novel Tyrosine-sulfated Chimera. J. Biol. Chem. 274: 27315-27322 [Abstract] [Full Text]  
• Kohrgruber, N., Halanek, N., Groger, M., Winter, D., Rappersberger, K., Schmitt-Egenolf, M., Stingl, G., Maurer, D. (1999). Survival, Maturation, and Function of CD11c- and CD11c+ Peripheral Blood Dendritic Cells Are Differentially Regulated by Cytokines. J. Immunol. 163: 3250-3259 [Abstract] [Full Text]  
• Sorg, R. V., Kogler, G., Wernet, P. (1999). Identification of Cord Blood Dendritic Cells as an Immature CD11c- Population. Blood 93: 2302-2307 [Abstract] [Full Text]  
• Wubbolts, R, Fernandez-Borja, M, Jordens, I, Reits, E, Dusseljee, S, Echeverri, C, Vallee, R., Neefjes, J (1999). Opposing motor activities of dynein and kinesin determine retention and transport of MHC class II-containing compartments. J. Cell Sci. 112: 785-795 [Abstract]  
• Hmama, Z., Gabathuler, R., Jefferies, W. A., de Jong, G., Reiner, N. E. (1998). Attenuation of HLA-DR Expression by Mononuclear Phagocytes Infected with Mycobacterium tuberculosis Is Related to Intracellular Sequestration of Immature Class II Heterodimers. J. Immunol. 161: 4882-4893 [Abstract] [Full Text]  
• Hammond, C., Denzin, L. K., Pan, M., Griffith, J. M., Geuze, H. J., Cresswell, P. (1998). The Tetraspan Protein CD82 Is a Resident of MHC Class II Compartments Where It Associates with HLA-DR, -DM, and -DO Molecules. J. Immunol. 161: 3282-3291 [Abstract] [Full Text]  
• Escola, J.-M., Kleijmeer, M. J., Stoorvogel, W., Griffith, J. M., Yoshie, O., Geuze, H. J. (1998). Selective Enrichment of Tetraspan Proteins on the Internal Vesicles of Multivesicular Endosomes and on Exosomes Secreted by Human B-lymphocytes. J. Biol. Chem. 273: 20121-20127 [Abstract] [Full Text]  
• Ahuja, S. S., Mummidi, S., Malech, H. L., Ahuja, S. K. (1998). Human Dendritic Cell (DC)-Based Anti-Infective Therapy: Engineering DCs to Secrete Functional IFN-{gamma} and IL-12. J. Immunol. 161: 868-876 [Abstract] [Full Text]  
• Rovere, P., Zimmermann, V. S., Forquet, F., Demandolx, D., Trucy, J., Ricciardi-Castagnoli, P., Davoust, J. (1998). Dendritic cell maturation and antigen presentation in the absence of invariant chain. Proc. Natl. Acad. Sci. USA 95: 1067-1072 [Abstract] [Full Text]  
• Raposo, G., Tenza, D., Mecheri, S., Peronet, R., Bonnerot, C., Desaymard, C. (1997). Accumulation of Major Histocompatibility Complex Class II Molecules in Mast Cell Secretory Granules and Their Release upon Degranulation. Mol. Biol. Cell 8: 2631-2645 [Abstract] [Full Text]  
• Kleijmeer, M. J., Morkowski, S., Griffith, J. M., Rudensky, A. Y., Geuze, H. J. (1997). Major Histocompatibility Complex Class II Compartments in Human and Mouse B Lymphoblasts Represent Conventional Endocytic Compartments. JCB 139: 639-649 [Abstract] [Full Text]  
• Hart, D. N.J. (1997). Dendritic Cells: Unique Leukocyte Populations Which Control the Primary Immune Response. Blood 90: 3245-3287 [Full Text]  
• Josien, R., Heslan, M., Soulillou, J.-P., Cuturi, M.-C. (1997). Rat Spleen Dendritic Cells Express Natural Killer Cell Receptor Protein 1 (NKR-P1) and Have Cytotoxic Activity to Select Targets via a Ca2+-dependent Mechanism. JEM 186: 467-472 [Abstract] [Full Text]  
• Fearnley, D.B., McLellan, A.D., Mannering, S.I., Hock, B.D., Hart, D.N.J. (1997). Isolation of Human Blood Dendritic Cells Using the CMRF-44 Monoclonal Antibody: Implications for Studies on Antigen-Presenting Cell Function and Immunotherapy. Blood 89: 3708-3716 [Abstract] [Full Text]  
• Denzin, L. K., Hammond, C., Cresswell, P. (1996). HLA-DM Interactions with Intermediates in HLA-DR Maturation and a Role for HLA-DM in Stabilizing Empty HLA-DR Molecules. JEM 184: 2153-2166 [Abstract] [Full Text]  
• Escola, J.-M., Deleuil, F., Stang, E., Boretto, J., Chavrier, P., Gorvel, J.-P. (1996). Characterization of a Lysozyme-Major Histocompatibility Complex Class II Molecule-loading Compartment as a Specialized Recycling Endosome in Murine B Lymphocytes. J. Biol. Chem. 271: 27360-27365 [Abstract] [Full Text]  
• Lim, T., Chen, G., McDonald, R., Toews, G. (1996). Granulocyte-macrophage colony-stimulating factor overrides the immunosuppressive function of corticosteroids on rat pulmonary dendritic cells. Stem Cells 14: 292-299 [Abstract]  
• Walter, W., Scheuer, C., Loos, M., Reichert, T. E., Maeurer, M. J. (2001). H2-Mbeta 1 and H2-Mbeta 2 Heterodimers Equally Promote CLIP Removal in I-Aq Molecules from Autoimmune-prone DBA/1 Mice. J. Biol. Chem. 276: 11086-11091 [Abstract] [Full Text]  
• Wilson, H. L., Ni, K., O'Neill, H. C. (2000). Identification of progenitor cells in long-term spleen stromal cultures that produce immature dendritic cells. Proc. Natl. Acad. Sci. USA 97: 4784-4789 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS