Journal of Experimental Medicine, Vol 180, 2413-2418, Copyright © 1994 by Rockefeller University Press

ARTICLES

Functional Myc-Max heterodimer is required for activation-induced apoptosis in T cell hybridomas

RP Bissonnette, A McGahon, A Mahboubi and DR Green
La Jolla Institute for Allergy and Immunology, California 92037.

T cell hybridomas respond to activation signals by undergoing apoptotic cell death, and this is likely to represent comparable events related to tolerance induction in immature and mature T cells in vivo. Previous studies using antisense oligonucleotides implicated the c-Myc protein in the phenomenon of activation-induced apoptosis. This role for c-Myc in apoptosis is now confirmed in studies using a dominant negative form of its heterodimeric binding partner, Max, which we show here inhibits activation-induced apoptosis. Further, coexpression of a reciprocally mutant Myc protein capable of forming functional heterodimers with the mutant Max can compensate for the dominant negative activity and restore activation-induced apoptosis. These results imply that Myc promotes activation-induced apoptosis by obligatory heterodimerization with Max, and therefore, by regulating gene transcription.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Yuan, Z.-R., Wang, R., Solomon, J., Luo, X., Sun, H., Zhang, L., Shi, Y. (2005). Identification and Characterization of Survival-Related Gene, a Novel Cell Survival Gene Controlling Apoptosis and Tumorigenesis. Cancer Res. 65: 10716-10724 [Abstract] [Full Text]  
• Li, Y., Xie, M., Song, X., Gragen, S., Sachdeva, K., Wan, Y., Yan, B. (2003). DEC1 Negatively Regulates the Expression of DEC2 through Binding to the E-box in the Proximal Promoter. J. Biol. Chem. 278: 16899-16907 [Abstract] [Full Text]  
• Song, R. X.-D., Mor, G., Naftolin, F., McPherson, R. A., Song, J., Zhang, Z., Yue, W., Wang, J., Santen, R. J. (2001). Effect of Long-Term Estrogen Deprivation on Apoptotic Responses of Breast Cancer Cells to 17{beta}-Estradiol. JNCI J Natl Cancer Inst 93: 1714-1723 [Abstract] [Full Text]  
• Delgado, M., Ganea, D. (2001). Vasoactive Intestinal Peptide and Pituitary Adenylate Cyclase-Activating Polypeptide Inhibit Expression of Fas Ligand in Activated T Lymphocytes by Regulating c-Myc, NF-{{kappa}}B, NF-AT, and Early Growth Factors 2/3. J. Immunol. 166: 1028-1040 [Abstract] [Full Text]  
• Brunner, T., Kasibhatla, S., Pinkoski, M. J., Frutschi, C., Yoo, N. J., Echeverri, F., Mahboubi, A., Green, D. R. (2000). Expression of Fas Ligand in Activated T Cells Is Regulated by c-Myc. J. Biol. Chem. 275: 9767-9772 [Abstract] [Full Text]  
• Genestier, L., Kasibhatla, S., Brunner, T., Green, D. R. (1999). Transforming Growth Factor beta 1 Inhibits Fas Ligand Expression and Subsequent Activation-induced Cell Death in T Cells via Downregulation of c-Myc. JEM 189: 231-239 [Abstract] [Full Text]  
• Hueber, A., Zörnig, M., Lyon, D., Suda, T., Nagata, S., Evan, G. I. (1997). Requirement for the CD95 Receptor-Ligand Pathway in c-Myc-Induced Apoptosis. Science 278: 1305-1309 [Abstract] [Full Text]  
• Lee, Y., Galoforo, S., Berns, C., Tong, W., Kim, H., Corry, P. (1997). Glucose deprivation-induced cytotoxicity in drug resistant human breast carcinoma MCF-7/ADR cells: role of c-myc and bcl-2 in apoptotic cell death. J. Cell Sci. 110: 681-686 [Abstract]  
• Wright, D. A., Futcher, B., Ghosh, P., Geha, R. S. (1996). Association of Human Fas (CD95) with a Ubiquitin-conjugating Enzyme (UBC-FAP). J. Biol. Chem. 271: 31037-31043 [Abstract] [Full Text]  
• Martin, S. J., O'Brien, G. A., Nishioka, W. K., McGahon, A. J., Mahboubi, A., Saido, T. C., Green, D. R. (1995). Proteolysis of Fodrin (Non-erythroid Spectrin) during Apoptosis. J. Biol. Chem. 270: 6425-6428 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS