Journal of Experimental Medicine, Vol 180, 1973-1978, Copyright © 1994 by Rockefeller University Press

ARTICLES

Persistence of human T cell lymphotropic virus type 1 (HTLV-1) sequences in peripheral blood mononuclear cells from patients with mycosis fungoides [published erratum appears in J Exp Med 1995 Jan 1;181(1):441]

N Manca, E Piacentini, M Gelmi, P Calzavara, MA Manganoni, A Glukhov, F Gargiulo, M De Francesco, F Pirali and G De Panfilis
Institute of Microbiology of Brescia University, Spedali Civili of Brescia, Italy.

Mycosis fungoides (MF) is a rare form of cutaneous T cell lymphoma suspected of having a viral etiology. As in adult T cell leukemia, the virus involved may be human T lymphotropic virus type 1 (HTLV-1). We cultured the peripheral blood mononuclear cells (PBMC) of 29 patients with MF HTLV-1 seronegative by enzyme-linked immunosorbent assay and Western blot. The presence of reverse transcriptase (RT) and p24 antigen was investigated in the concentrate supernatant of the culture. The DNA of all studied patients was submitted to polymerase chain reaction and Southern blot analysis using primers and probes recognizing the tax region of HTLV-1/2 and the pol region of HTLV-1. 10 of 29 patients were found positive to HTLV-1, whereas they were always negative to RT and p24. The same results were confirmed in double blind after 6 mo. Our findings suggest HTLV-1 may be involved in the etiology of MF, at least in certain cases.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• De Francesco, M. A., Gargiulo, F., Esteban, P., Calzavara-Pinton, P. G., Venturini, M., Perandin, F., Baronio, M., Pollara, C., Terlenghi, L., Manca, N. (2004). Polymorphism analysis of Epstein-Barr virus isolates of lymphoblastoid cell lines from patients with mycosis fungoides. J Med Microbiol 53: 381-387 [Abstract] [Full Text]  
• Nielsen, M., Nissen, M. H., Gerwien, J., Zocca, M.-B., Rasmussen, H. M., Nakajima, K., Ropke, C., Geisler, C., Kaltoft, K., Odum, N. (2002). Spontaneous interleukin-5 production in cutaneous T-cell lymphoma lines is mediated by constitutively activated Stat3. Blood 99: 973-977 [Abstract] [Full Text]  
• Koya, Y., Ohashi, T., Kato, H., Hanabuchi, S., Tsukahara, T., Takemura, F., Etoh, K.-i., Matsuoka, M., Fujii, M., Kannagi, M. (1999). Establishment of a Seronegative Human T-Cell Leukemia Virus Type 1 (HTLV-1) Carrier State in Rats Inoculated with a Syngeneic HTLV-1-Immortalized T-Cell Line Preferentially Expressing Tax. J. Virol. 73: 6436-6443 [Abstract] [Full Text]  
• Zucker-Franklin, D., Kosann, M. K., Pancake, B. A., Ramsay, D. L., Soter, N. A. (1999). Hypopigmented Mycosis Fungoides Associated With Human T Cell Lymphotropic Virus Type I Tax in a Pediatric Patient. Pediatrics 103: 1039-1044 [Full Text]  
• Nielsen, M., Kaltoft, K., Nordahl, M., Ropke, C., Geisler, C., Mustelin, T., Dobson, P., Svejgaard, A., Odum, N. (1997). Constitutive activation of a slowly migrating isoform of Stat3 in mycosis fungoides: Tyrphostin AG490 inhibits Stat3 activation and growth of mycosis fungoides tumor cell lines. Proc. Natl. Acad. Sci. USA 94: 6764-6769 [Abstract] [Full Text]  
• Kikuchi, A., Nishikawa, T., Ikeda, Y., Yamaguchi, K. (1997). Absence of Human T-Lymphotropic Virus Type I in Japanese Patients With Cutaneous T-Cell Lymphoma. Blood 89: 1529-1532 [Abstract] [Full Text]  
• Kikuchi, A., Nishikawa, T., Yamaguchi, K. (1997). Absence of Human T-Cell Lymphotropic Virus Type I in Cutaneous T-Cell Lymphoma. NEJM 336: 296-297 [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS