Evidence for a functional interaction between the beta chain of major histocompatibility complex class II and the T cell receptor alpha chain during recognition of a bacterial superantigen

doi:10.1084/jem.180.5.1931

This Article

	Full Text (PDF, 484K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Deckhut, A. M.
	Articles by Woodland, D. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Deckhut, A. M.
	Articles by Woodland, D. L.


Social Bookmarking

	What's this?

ARTICLES

Evidence for a functional interaction between the beta chain of major histocompatibility complex class II and the T cell receptor alpha chain during recognition of a bacterial superantigen

AM Deckhut, Y Chien, MA Blackman and DL Woodland
Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105.

Several studies have suggested that there is a direct interaction between the T cell receptor (TCR) and the major histocompatibility complex (MHC) molecule during T cell recognition of superantigen. To further investigate this possibility, we have analyzed T cell recognition of a bacterial superantigen, Staphylococcal enterotoxin B (SEB), presented by a series of mutant murine I-Ek molecules in which residues of either the alpha or beta chain predicted to interact with the TCR have been substituted. Individual T cell hybridomas gave distinct patterns of responsiveness to SEB presented by the I-E beta k mutants that could not be attributed to differences in the binding of SEB to the mutants. This effect appeared to be dependent on the TCR- alpha chain because some of these hybridomas expressed identical TCR transgenic beta chains. In contrast, none of the hybridomas gave distinct patterns of responsiveness to SEB presented by the I-E alpha k mutants. Taken together, these observations support the idea that there is a functional interaction between the alpha chain of the TCR and the beta chain of the MHC class II molecule. The data also support the idea that this interaction might enhance superantigen recognition in some cases.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Petersson, K., Pettersson, H., Skartved, N. J., Walse, B., Forsberg, G. (2003). Staphylococcal Enterotoxin H Induces V{alpha}-Specific Expansion of T Cells. J. Immunol. 170: 4148-4154 [Abstract] [Full Text]
Hogan, R. J., VanBeek, J., Broussard, D. R., Surman, S. L., Woodland, D. L. (2001). Identification of MHC Class II-Associated Peptides That Promote the Presentation of Toxic Shock Syndrome Toxin-1 to T Cells. J. Immunol. 166: 6514-6522 [Abstract] [Full Text]
Nilsson, H., Bjork, P., Dohlsten, M., Antonsson, P. (1999). Staphylococcal Enterotoxin H Displays Unique MHC Class II-Binding Properties. J. Immunol. 163: 6686-6693 [Abstract] [Full Text]
Reinherz, E. L., Tan, K., Tang, L., Kern, P., Liu, J., Xiong, Y., Hussey, R. E., Smolyar, A., Hare, B., Zhang, R., Joachimiak, A., Chang, H., Wagner, G., Wang, J. (1999). The Crystal Structure of a T Cell Receptor in Complex with Peptide and MHC Class II. Science 286: 1913-1921 [Abstract] [Full Text]
Blish, C. A., Gallay, B. J., Turk, G. L., Kline, K. M., Wheat, W., Fink, P. J. (1999). Chronic Modulation of the TCR Repertoire in the Lymphoid Periphery. J. Immunol. 162: 3131-3140 [Abstract] [Full Text]
Lamphear, J. G., Bohach, G. A., Rich, R. R. (1998). Structural Dichotomy of Staphylococcal Enterotoxin C Superantigens Leading to MHC Class II-Independent Activation of T Lymphocytes. J. Immunol. 160: 2107-2114 [Abstract] [Full Text]
Khandekar, S. S., Bettencourt, B. M., Wyss, D. F., Naylor, J. W., Brauer, P. P., Huestis, K., Dwyer, D. S., Profy, A. T., Osburne, M. S., Banerji, J., Jones, B. (1997). Conformational Integrity and Ligand Binding Properties of a Single Chain T-cell Receptor Expressed in Escherichia coli. J. Biol. Chem. 272: 32190-32197 [Abstract] [Full Text]
Cho, B. K., Schodin, B. A., Kranz, D. M. (1995). Characterization of a Single-chain Antibody to the beta-Chain of the T Cell Receptor. J. Biol. Chem. 270: 25819-25826 [Abstract] [Full Text]