Altered glycosylation of leukosialin, CD43, in HIV-1-infected cells of the CEM line

doi:10.1084/jem.180.5.1609

BioLegend: PerCP, PerCP/Cy5.5 Ab Conjugates

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Lefebvre, J. C.
	Articles by Peyron, J. F.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Lefebvre, J. C.
	Articles by Peyron, J. F.


Social Bookmarking

	What's this?

ARTICLES

Altered glycosylation of leukosialin, CD43, in HIV-1-infected cells of the CEM line

JC Lefebvre, V Giordanengo, M Limouse, A Doglio, M Cucchiarini, F Monpoux, R Mariani and JF Peyron
Laboratoire de Virologie, Faculte de Medecine, Hopital de Cimiez, France.

CD43 (leukosialin, gpL115, sialophorin) is a major sialoglycoprotein widely expressed on hematopoietic cells that is defective in the congenital immunodeficiency Wiskott-Aldrich syndrome. It is thought to play an important role in cell-cell interactions and to be a costimulatory molecule for T lymphocyte activation. Using a metabolic 35SO4(2-) radiolabeling assay or biotinylation of cell surface proteins, we describe here that CD43 are sulfated molecules the glycosylation of which is altered in human immunodeficiency virus type 1 (HIV-1)-infected leukemic T cells of the CEM line. Hyposialylation of O-glycans and changed substitution on N-acetylgalactosamine residues are observed. The glycosylation defect is associated with an impairment of CD43-mediated homotypic aggregation which can be restored by resialylation. The hyposialylation of CD43 on HIV-1+ cells may explain the high prevalence of autoantibodies directed against nonsialylated CD43 that have been detected in HIV-1-infected individuals. A defect in glycosylation of important molecules such as CD43 or, as we recently described, CD45 may explain alterations of T cell functions and viability in HIV-1-infected individuals. In addition, a possible implication of hyposialylation in the HIV-1-infected cells entrapment in lymph nodes could be envisioned.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Mody, P. D., Cannon, J. L., Bandukwala, H. S., Blaine, K. M., Schilling, A. B., Swier, K., Sperling, A. I. (2007). Signaling through CD43 regulates CD4 T-cell trafficking. Blood 110: 2974-2982 [Abstract] [Full Text]
Markine-Goriaynoff, N., Gillet, L., Karlsen, O. A., Haarr, L., Minner, F., Pastoret, P.-P., Fukuda, M., Vanderplasschen, A. (2004). The core 2 {beta}-1,6-N-acetylglucosaminyltransferase-M encoded by bovine herpesvirus 4 is not essential for virus replication despite contributing to post-translational modifications of structural proteins. J. Gen. Virol. 85: 355-367 [Abstract] [Full Text]
Lanteri, M., Giordanengo, V., Hiraoka, N., Fuzibet, J.-G., Auberger, P., Fukuda, M., Baum, L. G., Lefebvre, J.-C. (2003). Altered T cell surface glycosylation in HIV-1 infection results in increased susceptibility to galectin-1-induced cell death. Glycobiology 13: 909-918 [Abstract] [Full Text]
Falkenberg, V. R., Alvarez, K., Roman, C., Fregien, N. (2003). Multiple transcription initiation and alternative splicing in the 5' untranslated region of the core 2 {beta}1-6 N-acetylglucosaminyltransferase I gene. Glycobiology 13: 411-418 [Abstract] [Full Text]
Lanteri, M., Giordanengo, V., Vidal, F., Gaudray, P., Lefebvre, J.-C. (2002). A complete {alpha}1,3-galactosyltransferase gene is present in the human genome and partially transcribed. Glycobiology 12: 785-792 [Abstract] [Full Text]
Da Silva, N., Bharti, A., Shelley, C. S. (2002). hnRNP-K and Puralpha act together to repress the transcriptional activity of the CD43 gene promoter. Blood 100: 3536-3544 [Abstract] [Full Text]
Barat, C., Tremblay, M. J. (2002). Engagement of CD43 Enhances Human Immunodeficiency Virus Type 1 Transcriptional Activity and Virus Production That Is Induced upon TCR/CD3 Stimulation. J. Biol. Chem. 277: 28714-28724 [Abstract] [Full Text]
Cermak, L., Simova, S., Pintzas, A., Horejsi, V., Andera, L. (2002). Molecular Mechanisms Involved in CD43-mediated Apoptosis of TF-1 Cells. ROLES OF TRANSCRIPTION, Daxx EXPRESSION, AND ADHESION MOLECULES. J. Biol. Chem. 277: 7955-7961 [Abstract] [Full Text]
Nguyen, J. T., Evans, D. P., Galvan, M., Pace, K. E., Leitenberg, D., Bui, T. N., Baum, L. G. (2001). CD45 Modulates Galectin-1-Induced T Cell Death: Regulation by Expression of Core 2 O-Glycans. J. Immunol. 167: 5697-5707 [Abstract] [Full Text]
Carlow, D. A., Ardman, B., Ziltener, H. J. (1999). A Novel CD8 T Cell-Restricted CD45RB Epitope Shared by CD43 Is Differentially Affected by Glycosylation. J. Immunol. 163: 1441-1448 [Abstract] [Full Text]
Ujita, M., McAuliffe, J., Schwientek, T., Almeida, R., Hindsgaul, O., Clausen, H., Fukuda, M. (1998). Synthesis of Poly-N-acetyllactosamine in Core 2�Branched O-Glycans. THE REQUIREMENT OF NOVEL beta -1,4-GALACTOSYLTRANSFERASE IV AND beta -1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE. J. Biol. Chem. 273: 34843-34849 [Abstract] [Full Text]
Tsuboi, S., Fukuda, M. (1998). Overexpression of Branched O-Linked Oligosaccharides on T Cell Surface Glycoproteins Impairs Humoral Immune Responses in Transgenic Mice. J. Biol. Chem. 273: 30680-30687 [Abstract] [Full Text]
Bannwarth, S., Giordanengo, V., Lesimple, J., Lefebvre, J.-C. (1998). Molecular Cloning of a New Secreted Sulfated Mucin-like Protein With a C-type Lectin Domain That Is Expressed in Lymphoblastic Cells. J. Biol. Chem. 273: 1911-1916 [Abstract] [Full Text]
Skrincosky, D., Kain, R., El-Battari, A., Exner, M., Kerjaschki, D., Fukuda, M. (1997). Altered Golgi Localization of Core 2�beta -1,6-N-Acetylglucosaminyltransferase Leads to Decreased Synthesis of Branched O-Glycans. J. Biol. Chem. 272: 22695-22702 [Abstract] [Full Text]
Brown, T. J., Shuford, W. W., Wang, W.-C., Nadler, S. G., Bailey, T. S., Marquardt, H., Mittler, R. S. (1996). Characterization of a CD43/Leukosialin-mediated Pathway for Inducing Apoptosis in Human T-Lymphoblastoid Cells. J. Biol. Chem. 271: 27686-27695 [Abstract] [Full Text]