Naturally processed viral peptides recognized by cytotoxic T lymphocytes on cells chronically infected by human immunodeficiency virus type 1

doi:10.1084/jem.180.4.1283

This Article

	Full Text (PDF, 2434K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Tsomides, T. J.
	Articles by Eisen, H. N.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Tsomides, T. J.
	Articles by Eisen, H. N.


Social Bookmarking

	What's this?

ARTICLES

Naturally processed viral peptides recognized by cytotoxic T lymphocytes on cells chronically infected by human immunodeficiency virus type 1

TJ Tsomides, A Aldovini, RP Johnson, BD Walker, RA Young and HN Eisen
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.

We have established long-term cultures of several cell lines stably and uniformly expressing human immunodeficiency virus type 1 (HIV-1) in order to (a) identify naturally processed HIV-1 peptides recognized by cytotoxic T lymphocytes (CTL) from HIV-1-seropositive individuals and (b) consider the hypothesis that naturally occurring epitope densities on HIV-infected cells may limit their lysis by CTL. Each of two A2- restricted CD8+ CTL specific for HIV-1 gag or reverse transcriptase (RT) recognized a single naturally processed HIV-1 peptide in trifluoroacetic acid (TFA) extracts of infected cells: gag 77-85 (SLYNTVATL) or RT 476-484 (ILKEPVHGV). Both processed peptides match the synthetic peptides that are optimally active in cytotoxicity assays and have the consensus motif described for A2-associated peptides. Their abundances were approximately 400 and approximately 12 molecules per infected Jurkat-A2 cell, respectively. Other synthetic HIV-1 peptides active at subnanomolar concentrations were not present in infected cells. Except for the antigen processing mutant line T2, HIV- infected HLA-A2+ cell lines were specifically lysed by both A2- restricted CTL, although infected Jurkat-A2 cells were lysed more poorly by RT-specific CTL than by gag-specific CTL, suggesting that low cell surface density of a natural peptide may limit the effectiveness of some HIV-specific CTL despite their vigorous activity against synthetic peptide-treated target cells.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Joshi, A., Tang, J., Kuzma, M., Wagner, J., Mookerjee, B., Filicko, J., Carabasi, M., Flomenberg, N., Flomenberg, P. (2009). Adenovirus DNA polymerase is recognized by human CD8+ T cells. J. Gen. Virol. 90: 84-94 [Abstract] [Full Text]
Varghese, J. C., Kane, K. P. (2008). TCR Complex-Activated CD8 Adhesion Function by Human T Cells. J. Immunol. 181: 6002-6009 [Abstract] [Full Text]
Calarota, S. A., Foli, A., Maserati, R., Baldanti, F., Paolucci, S., Young, M. A., Tsoukas, C. M., Lisziewicz, J., Lori, F. (2008). HIV-1-Specific T Cell Precursors with High Proliferative Capacity Correlate with Low Viremia and High CD4 Counts in Untreated Individuals. J. Immunol. 180: 5907-5915 [Abstract] [Full Text]
Almeida, J. R., Price, D. A., Papagno, L., Arkoub, Z. A., Sauce, D., Bornstein, E., Asher, T. E., Samri, A., Schnuriger, A., Theodorou, I., Costagliola, D., Rouzioux, C., Agut, H., Marcelin, A.-G., Douek, D., Autran, B., Appay, V. (2007). Superior control of HIV-1 replication by CD8+ T cells is reflected by their avidity, polyfunctionality, and clonal turnover. JEM 204: 2473-2485 [Abstract] [Full Text]
Schaubert, K. L., Price, D. A., Frahm, N., Li, J., Ng, H. L., Joseph, A., Paul, E., Majumder, B., Ayyavoo, V., Gostick, E., Adams, S., Marincola, F. M., Sewell, A. K., Altfeld, M., Brenchley, J. M., Douek, D. C., Yang, O. O., Brander, C., Goldstein, H., Kan-Mitchell, J. (2007). Availability of a Diversely Avid CD8+ T Cell Repertoire Specific for the Subdominant HLA-A2-Restricted HIV-1 Gag p2419-27 Epitope. J. Immunol. 178: 7756-7766 [Abstract] [Full Text]
Bennett, M. S., Ng, H. L., Dagarag, M., Ali, A., Yang, O. O. (2007). Epitope-Dependent Avidity Thresholds for Cytotoxic T-Lymphocyte Clearance of Virus-Infected Cells. J. Virol. 81: 4973-4980 [Abstract] [Full Text]
Sacha, J. B., Chung, C., Rakasz, E. G., Spencer, S. P., Jonas, A. K., Bean, A. T., Lee, W., Burwitz, B. J., Stephany, J. J., Loffredo, J. T., Allison, D. B., Adnan, S., Hoji, A., Wilson, N. A., Friedrich, T. C., Lifson, J. D., Yang, O. O., Watkins, D. I. (2007). Gag-Specific CD8+ T Lymphocytes Recognize Infected Cells before AIDS-Virus Integration and Viral Protein Expression. J. Immunol. 178: 2746-2754 [Abstract] [Full Text]
Adnan, S., Balamurugan, A., Trocha, A., Bennett, M. S., Ng, H. L., Ali, A., Brander, C., Yang, O. O. (2006). Nef interference with HIV-1-specific CTL antiviral activity is epitope specific. Blood 108: 3414-3419 [Abstract] [Full Text]
Anikeeva, N., Lebedeva, T., Clapp, A. R., Goldman, E. R., Dustin, M. L., Mattoussi, H., Sykulev, Y. (2006). Quantum dot/peptide-MHC biosensors reveal strong CD8-dependent cooperation between self and viral antigens that augment the T cell response. Proc. Natl. Acad. Sci. USA 103: 16846-16851 [Abstract] [Full Text]
Sommerfeldt, N., Beckhove, P., Ge, Y., Schutz, F., Choi, C., Bucur, M., Domschke, C., Sohn, C., Schneeweis, A., Rom, J., Pollmann, D., Leucht, D., Vlodavsky, I., Schirrmacher, V. (2006). Heparanase: a new metastasis-associated antigen recognized in breast cancer patients by spontaneously induced memory T lymphocytes.. Cancer Res. 66: 7716-7723 [Abstract] [Full Text]
Kan-Mitchell, J., Bajcz, M., Schaubert, K. L., Price, D. A., Brenchley, J. M., Asher, T. E., Douek, D. C., Ng, H. L., Yang, O. O., Rinaldo, C. R. Jr., Benito, J. M., Bisikirska, B., Hegde, R., Marincola, F. M., Boggiano, C., Wilson, D., Abrams, J., Blondelle, S. E., Wilson, D. B. (2006). Degeneracy and Repertoire of the Human HIV-1 Gag p1777-85 CTL Response.. J. Immunol. 176: 6690-6701 [Abstract] [Full Text]
Margalit, A., Sheikhet, H. M., Carmi, Y., Berko, D., Tzehoval, E., Eisenbach, L., Gross, G. (2006). Induction of Antitumor Immunity by CTL Epitopes Genetically Linked to Membrane-Anchored {beta}2-Microglobulin. J. Immunol. 176: 217-224 [Abstract] [Full Text]
Anikeeva, N., Somersalo, K., Sims, T. N., Thomas, V. K., Dustin, M. L., Sykulev, Y. (2005). Distinct role of lymphocyte function-associated antigen-1 in mediating effective cytolytic activity by cytotoxic T lymphocytes. Proc. Natl. Acad. Sci. USA 102: 6437-6442 [Abstract] [Full Text]
Huang, X.-L., Fan, Z., Colleton, B. A., Buchli, R., Li, H., Hildebrand, W. H., Rinaldo, C. R. Jr. (2005). Processing and Presentation of Exogenous HLA Class I Peptides by Dendritic Cells from Human Immunodeficiency Virus Type 1-Infected Persons. J. Virol. 79: 3052-3062 [Abstract] [Full Text]
Choi, C., Witzens, M., Bucur, M., Feuerer, M., Sommerfeldt, N., Trojan, A., Ho, A., Schirrmacher, V., Goldschmidt, H., Beckhove, P. (2005). Enrichment of functional CD8 memory T cells specific for MUC1 in bone marrow of patients with multiple myeloma. Blood 105: 2132-2134 [Abstract] [Full Text]
Du Pasquier, R. A., Schmitz, J. E., Jean-Jacques, J., Zheng, Y., Gordon, J., Khalili, K., Letvin, N. L., Koralnik, I. J. (2004). Detection of JC Virus-Specific Cytotoxic T Lymphocytes in Healthy Individuals. J. Virol. 78: 10206-10210 [Abstract] [Full Text]
Du Pasquier, R. A., Kuroda, M. J., Zheng, Y., Jean-Jacques, J., Letvin, N. L., Koralnik, I. J. (2004). A prospective study demonstrates an association between JC virus-specific cytotoxic T lymphocytes and the early control of progressive multifocal leukoencephalopathy. Brain 127: 1970-1978 [Abstract] [Full Text]
Ferrari, G., Neal, W., Ottinger, J., Jones, A. M., Edwards, B. H., Goepfert, P., Betts, M. R., Koup, R. A., Buchbinder, S., McElrath, M. J., Tartaglia, J., Weinhold, K. J. (2004). Absence of Immunodominant Anti-Gag p17 (SL9) Responses among Gag CTL-Positive, HIV-Uninfected Vaccine Recipients Expressing the HLA-A*0201 Allele. J. Immunol. 173: 2126-2133 [Abstract] [Full Text]
Betts, M. R., Price, D. A., Brenchley, J. M., Lore, K., Guenaga, F. J., Smed-Sorensen, A., Ambrozak, D. R., Migueles, S. A., Connors, M., Roederer, M., Douek, D. C., Koup, R. A. (2004). The Functional Profile of Primary Human Antiviral CD8+ T Cell Effector Activity Is Dictated by Cognate Peptide Concentration. J. Immunol. 172: 6407-6417 [Abstract] [Full Text]
Kan-Mitchell, J., Bisikirska, B., Wong-Staal, F., Schaubert, K. L., Bajcz, M., Bereta, M. (2004). The HIV-1 HLA-A2-SLYNTVATL Is a Help-Independent CTL Epitope. J. Immunol. 172: 5249-5261 [Abstract] [Full Text]
Wang, R., Epstein, J., Charoenvit, Y., Baraceros, F. M., Rahardjo, N., Gay, T., Banania, J.-G., Chattopadhyay, R., de la Vega, P., Richie, T. L., Tornieporth, N., Doolan, D. L., Kester, K. E., Heppner, D. G., Norman, J., Carucci, D. J., Cohen, J. D., Hoffman, S. L. (2004). Induction in Humans of CD8+ and CD4+ T Cell and Antibody Responses by Sequential Immunization with Malaria DNA and Recombinant Protein. J. Immunol. 172: 5561-5569 [Abstract] [Full Text]
Moris, A., Nobile, C., Buseyne, F., Porrot, F., Abastado, J.-P., Schwartz, O. (2004). DC-SIGN promotes exogenous MHC-I-restricted HIV-1 antigen presentation. Blood 103: 2648-2654 [Abstract] [Full Text]
Yokomaku, Y., Miura, H., Tomiyama, H., Kawana-Tachikawa, A., Takiguchi, M., Kojima, A., Nagai, Y., Iwamoto, A., Matsuda, Z., Ariyoshi, K. (2004). Impaired Processing and Presentation of Cytotoxic-T-Lymphocyte (CTL) Epitopes Are Major Escape Mechanisms from CTL Immune Pressure in Human Immunodeficiency Virus Type 1 Infection. J. Virol. 78: 1324-1332 [Abstract] [Full Text]
Ali, A., Lubong, R., Ng, H., Brooks, D. G., Zack, J. A., Yang, O. O. (2004). Impacts of Epitope Expression Kinetics and Class I Downregulation on the Antiviral Activity of Human Immunodeficiency Virus Type 1-Specific Cytotoxic T Lymphocytes. J. Virol. 78: 561-567 [Abstract] [Full Text]
Samino, Y., Lopez, D., Guil, S., de Leon, P., Del Val, M. (2004). An Endogenous HIV Envelope-derived Peptide without the Terminal NH3+ Group Anchor Is Physiologically Presented by Major Histocompatibility Complex Class I Molecules. J. Biol. Chem. 279: 1151-1160 [Abstract] [Full Text]
Jamieson, B. D., Yang, O. O., Hultin, L., Hausner, M. A., Hultin, P., Matud, J., Kunstman, K., Killian, S., Altman, J., Kommander, K., Korber, B., Giorgi, J., Wolinsky, S. (2003). Epitope Escape Mutation and Decay of Human Immunodeficiency Virus Type 1-Specific CTL Responses. J. Immunol. 171: 5372-5379 [Abstract] [Full Text]
Smith, P. D., Meng, G., Salazar-Gonzalez, J. F., Shaw, G. M. (2003). Macrophage HIV-1 infection and the gastrointestinal tract reservoir. J. Leukoc. Biol. 74: 642-649 [Abstract] [Full Text]
Daftarian, P., Ali, S., Sharan, R., Lacey, S. F., La Rosa, C., Longmate, J., Buck, C., Siliciano, R. F., Diamond, D. J. (2003). Immunization with Th-CTL Fusion Peptide and Cytosine-Phosphate-Guanine DNA in Transgenic HLA-A2 Mice Induces Recognition of HIV-Infected T Cells and Clears Vaccinia Virus Challenge. J. Immunol. 171: 4028-4039 [Abstract] [Full Text]
Yang, O. O., Sarkis, P. T. N., Trocha, A., Kalams, S. A., Johnson, R. P., Walker, B. D. (2003). Impacts of Avidity and Specificity on the Antiviral Efficiency of HIV-1-Specific CTL. J. Immunol. 171: 3718-3724 [Abstract] [Full Text]
Lucchiari-Hartz, M., Lindo, V., Hitziger, N., Gaedicke, S., Saveanu, L., van Endert, P. M., Greer, F., Eichmann, K., Niedermann, G. (2003). Differential proteasomal processing of hydrophobic and hydrophilic protein regions: Contribution to cytotoxic T lymphocyte epitope clustering in HIV-1-Nef. Proc. Natl. Acad. Sci. USA 100: 7755-7760 [Abstract] [Full Text]
Dagarag, M., Ng, H., Lubong, R., Effros, R. B., Yang, O. O. (2003). Differential Impairment of Lytic and Cytokine Functions in Senescent Human Immunodeficiency Virus Type 1-Specific Cytotoxic T Lymphocytes. J. Virol. 77: 3077-3083 [Abstract] [Full Text]
Cohen, W. M., Bianco, A., Connan, F., Camoin, L., Dalod, M., Lauvau, G., Ferries, E., Culmann-Penciolelli, B., van Endert, P. M., Briand, J. P., Choppin, J., Guillet, J. G. (2002). Study of Antigen-Processing Steps Reveals Preferences Explaining Differential Biological Outcomes of Two HLA-A2-Restricted Immunodominant Epitopes from Human Immunodeficiency Virus Type 1. J. Virol. 76: 10219-10225 [Abstract] [Full Text]
DiPaolo, R. J., Unanue, E. R. (2002). Cutting Edge: Chemical Dominance Does Not Relate to Immunodominance: Studies of the CD4+ T Cell Response to a Model Antigen. J. Immunol. 169: 1-4 [Abstract] [Full Text]
Alexander, J., Oseroff, C., Dahlberg, C., Qin, M., Ishioka, G., Beebe, M., Fikes, J., Newman, M., Chesnut, R. W., Morton, P. A., Fok, K., Appella, E., Sette, A. (2002). A Decaepitope Polypeptide Primes for Multiple CD8+ IFN-{gamma} and Th Lymphocyte Responses: Evaluation of Multiepitope Polypeptides as a Mode for Vaccine Delivery. J. Immunol. 168: 6189-6198 [Abstract] [Full Text]
von Bergwelt-Baildon, M. S., Vonderheide, R. H., Maecker, B., Hirano, N., Anderson, K. S., Butler, M. O., Xia, Z., Zeng, W. Y., Wucherpfennig, K. W., Nadler, L. M., Schultze, J. L. (2002). Human primary and memory cytotoxic T lymphocyte responses are efficiently induced by means of CD40-activated B cells as antigen-presenting cells: potential for clinical application. Blood 99: 3319-3325 [Abstract] [Full Text]
Lieberman, J., Shankar, P., Manjunath, N., Andersson, J. (2001). Dressed to kill? A review of why antiviral CD8 T lymphocytes fail to prevent progressive immunodeficiency in HIV-1 infection. Blood 98: 1667-1677 [Abstract] [Full Text]
Herberts, C. A., Stittelaar, K. J., van der Heeft, E., van Gaans-van den Brink, J., Poelen, M. C. M., Roholl, P. J. M., van Alphen, L. J. W., Melief, C. J. M., de Jong, A. P. J. M., van Els, C. A. C. M. (2001). A measles virus glycoprotein-derived human CTL epitope is abundantly presented via the proteasomal-dependent MHC class I processing pathway. J. Gen. Virol. 82: 2131-2142 [Abstract] [Full Text]
Wang, R., Epstein, J., Baraceros, F. M., Gorak, E. J., Charoenvit, Y., Carucci, D. J., Hedstrom, R. C., Rahardjo, N., Gay, T., Hobart, P., Stout, R., Jones, T. R., Richie, T. L., Parker, S. E., Doolan, D. L., Norman, J., Hoffman, S. L. (2001). Induction of CD4+ T cell-dependent CD8+ type 1 responses in humans by a malaria DNA vaccine. Proc. Natl. Acad. Sci. USA 10.1073/pnas.181123498v1 [Abstract] [Full Text]
Day, C. L., Shea, A. K., Altfeld, M. A., Olson, D. P., Buchbinder, S. P., Hecht, F. M., Rosenberg, E. S., Walker, B. D., Kalams, S. A. (2001). Relative Dominance of Epitope-Specific Cytotoxic T-Lymphocyte Responses in Human Immunodeficiency Virus Type 1-Infected Persons with Shared HLA Alleles. J. Virol. 75: 6279-6291 [Abstract] [Full Text]
Velazquez, C., DiPaolo, R., Unanue, E. R. (2001). Quantitation of Lysozyme Peptides Bound to Class II MHC Molecules Indicates Very Large Differences in Levels of Presentation. J. Immunol. 166: 5488-5494 [Abstract] [Full Text]
Goulder, P. J.R., Altfeld, M. A., Rosenberg, E. S., Nguyen, T., Tang, Y., Eldridge, R. L., Addo, M. M., He, S., Mukherjee, J. S., Phillips, M. N., Bunce, M., Kalams, S. A., Sekaly, R. P., Walker, B. D., Brander, C. (2001). Substantial Differences in Specificity of HIV-specific Cytotoxic T Cells in Acute and Chronic HIV Infection. JEM 193: 181-194 [Abstract] [Full Text]
Goulder, P. J.R., Tang, Y., Brander, C., Betts, M. R., Altfeld, M., Annamalai, K., Trocha, A., He, S., Rosenberg, E. S., Ogg, G., O'Callaghan, C. A., Kalams, S. A., McKinney, R. E. , Jr., Mayer, K., Koup, R. A., Pelton, S. I., Burchett, S. K., McIntosh, K., Walker, B. D. (2000). Functionally Inert HIV-specific Cytotoxic T Lymphocytes Do Not Play a Major Role in Chronically Infected Adults and Children. JEM 192: 1819-1832 [Abstract] [Full Text]
Goepfert, P. A., Bansal, A., Edwards, B. H., Ritter, G. D. Jr., Tellez, I., McPherson, S. A., Sabbaj, S., Mulligan, M. J. (2000). A Significant Number of Human Immunodeficiency Virus Epitope-Specific Cytotoxic T Lymphocytes Detected by Tetramer Binding Do Not Produce Gamma Interferon. J. Virol. 74: 10249-10255 [Abstract] [Full Text]
Le Gall, S., Buseyne, F., Trocha, A., Walker, B. D., Heard, J.-M., Schwartz, O. (2000). Distinct Trafficking Pathways Mediate Nef-Induced and Clathrin-Dependent Major Histocompatibility Complex Class I Down-Regulation. J. Virol. 74: 9256-9266 [Abstract] [Full Text]
Samri, A., Haas, G., Duntze, J., Bouley, J.-M., Calvez, V., Katlama, C., Autran, B. (2000). Immunogenicity of Mutations Induced by Nucleoside Reverse Transcriptase Inhibitors for Human Immunodeficiency Virus Type 1-Specific Cytotoxic T Cells. J. Virol. 74: 9306-9312 [Abstract] [Full Text]
Dela Cruz, C. S., Tan, R., Rowland-Jones, S. L., Barber, B. H. (2000). Creating HIV-1 reverse transcriptase cytotoxic T lymphocyte target structures by HLA-A2 heavy chain modifications. Int Immunol 12: 1293-1302 [Abstract] [Full Text]
Trimble, L. A., Shankar, P., Patterson, M., Daily, J. P., Lieberman, J. (2000). Human Immunodeficiency Virus-Specific Circulating CD8 T Lymphocytes Have Down-Modulated CD3zeta and CD28, Key Signaling Molecules for T-Cell Activation. J. Virol. 74: 7320-7330 [Abstract] [Full Text]
Mollet, L., Li, T.-S., Samri, A., Tournay, C., Tubiana, R., Calvez, V., Debre, P., Katlama, C., Autran, B. (2000). Dynamics of HIV-Specific CD8+ T Lymphocytes with Changes in Viral Load. J. Immunol. 165: 1692-1704 [Abstract] [Full Text]
Goulder, P. J. R., Brander, C., Annamalai, K., Mngqundaniso, N., Govender, U., Tang, Y., He, S., Hartman, K. E., O'Callaghan, C. A., Ogg, G. S., Altfeld, M. A., Rosenberg, E. S., Cao, H., Kalams, S. A., Hammond, M., Bunce, M., Pelton, S. I., Burchett, S. A., McIntosh, K., Coovadia, H. M., Walker, B. D. (2000). Differential Narrow Focusing of Immunodominant Human Immunodeficiency Virus Gag-Specific Cytotoxic T-Lymphocyte Responses in Infected African and Caucasoid Adults and Children. J. Virol. 74: 5679-5690 [Abstract] [Full Text]
Crotzer, V. L., Christian, R. E., Brooks, J. M., Shabanowitz, J., Settlage, R. E., Marto, J. A., White, F. M., Rickinson, A. B., Hunt, D. F., Engelhard, V. H. (2000). Immunodominance Among EBV-Derived Epitopes Restricted by HLA-B27 Does Not Correlate with Epitope Abundance in EBV-Transformed B-Lymphoblastoid Cell Lines. J. Immunol. 164: 6120-6129 [Abstract] [Full Text]
Iroegbu, J., Birk, M., Lazdina, U., Sonnerborg, A., Sallberg, M. (2000). Variability and Immunogenicity of Human Immunodeficiency Virus Type 1 p24 Gene Quasispecies. CVI 7: 377-383 [Abstract] [Full Text]
Rinaldo, C. R. Jr., Huang, X.-L., Fan, Z., Margolick, J. B., Borowski, L., Hoji, A., Kalinyak, C., McMahon, D. K., Riddler, S. A., Hildebrand, W. H., Day, R. B., Mellors, J. W. (2000). Anti-Human Immunodeficiency Virus Type 1 (HIV-1) CD8+ T-Lymphocyte Reactivity during Combination Antiretroviral Therapy in HIV-1-Infected Patients with Advanced Immunodeficiency. J. Virol. 74: 4127-4138 [Abstract] [Full Text]
Huang, X.-L., Fan, Z., Kalinyak, C., Mellors, J. W., Rinaldo, C. R. Jr. (2000). CD8+ T-Cell Gamma Interferon Production Specific for Human Immunodeficiency Virus Type 1 (HIV-1) in HIV-1-Infected Subjects. CVI 7: 279-287 [Abstract] [Full Text]
Lucchiari-Hartz, M., van Endert, P. M., Lauvau, G., Maier, R., Meyerhans, A., Mann, D., Eichmann, K., Niedermann, G. (2000). Cytotoxic T Lymphocyte Epitopes of HIV-1 Nef: Generation of Multiple Definitive Major Histocompatibility Complex Class I Ligands by Proteasomes. JEM 191: 239-252 [Abstract] [Full Text]
Ogg, G. S., Kostense, S., Klein, M. R., Jurriaans, S., Hamann, D., McMichael, A. J., Miedema, F. (1999). Longitudinal Phenotypic Analysis of Human Immunodeficiency Virus Type 1-Specific Cytotoxic T Lymphocytes: Correlation with Disease Progression. J. Virol. 73: 9153-9160 [Abstract] [Full Text]
Shankar, P., Xu, Z., Lieberman, J. (1999). Viral-Specific Cytotoxic T Lymphocytes Lyse Human Immunodeficiency Virus-Infected Primary T Lymphocytes by the Granule Exocytosis Pathway. Blood 94: 3084-3093 [Abstract] [Full Text]
Kalams, S. A., Buchbinder, S. P., Rosenberg, E. S., Billingsley, J. M., Colbert, D. S., Jones, N. G., Shea, A. K., Trocha, A. K., Walker, B. D. (1999). Association between Virus-Specific Cytotoxic T-Lymphocyte and Helper Responses in Human Immunodeficiency Virus Type 1 Infection. J. Virol. 73: 6715-6720 [Abstract] [Full Text]
Hay, C. M., Ruhl, D. J., Basgoz, N. O., Wilson, C. C., Billingsley, J. M., DePasquale, M. P., D'Aquila, R. T., Wolinsky, S. M., Crawford, J. M., Montefiori, D. C., Walker, B. D. (1999). Lack of Viral Escape and Defective In Vivo Activation of Human Immunodeficiency Virus Type 1-Specific Cytotoxic T Lymphocytes in Rapidly Progressive Infection. J. Virol. 73: 5509-5519 [Abstract] [Full Text]
Sewell, A. K., Price, D. A., Teisserenc, H., Booth, B. L. Jr., Gileadi, U., Flavin, F. M., Trowsdale, J., Phillips, R. E., Cerundolo, V. (1999). IFN-{gamma} Exposes a Cryptic Cytotoxic T Lymphocyte Epitope in HIV-1 Reverse Transcriptase. J. Immunol. 162: 7075-7079 [Abstract] [Full Text]
Prilliman, K. R., Jackson, K. W., Lindsey, M., Wang, J., Crawford, D., Hildebrand, W. H. (1999). HLA-B15 Peptide Ligands Are Preferentially Anchored at Their C Termini. J. Immunol. 162: 7277-7284 [Abstract] [Full Text]
Ishioka, G. Y., Fikes, J., Hermanson, G., Livingston, B., Crimi, C., Qin, M., France del Guercio, M., Oseroff, C., Dahlberg, C., Alexander, J., Chesnut, R. W., Sette, A. (1999). Utilization of MHC Class I Transgenic Mice for Development of Minigene DNA Vaccines Encoding Multiple HLA-Restricted CTL Epitopes. J. Immunol. 162: 3915-3925 [Abstract] [Full Text]
Wagner, R., Leschonsky, B., Harrer, E., Paulus, C., Weber, C., Walker, B. D., Buchbinder, S., Wolf, H., Kalden, J. R., Harrer, T. (1999). Molecular and Functional Analysis of a Conserved CTL Epitope in HIV-1 p24 Recognized from a Long-Term Nonprogressor: Constraints on Immune Escape Associated with Targeting a Sequence Essential for Viral Replication. J. Immunol. 162: 3727-3734 [Abstract] [Full Text]
Stuhler, G., Zobywalski, A., Grunebach, F., Brossart, P., Reichardt, V. L., Barth, H., Stevanovic, S., Brugger, W., Kanz, L., Schlossman, S. F. (1999). Immune regulatory loops determine productive interactions within human T lymphocyte-dendritic cell clusters. Proc. Natl. Acad. Sci. USA 96: 1532-1535 [Abstract] [Full Text]
Gray, C. M., Lawrence, J., Schapiro, J. M., Altman, J. D., Winters, M. A., Crompton, M., Loi, M., Kundu, S. K., Davis, M. M., Merigan, T. C. (1999). Frequency of Class I HLA-Restricted Anti-HIV CD8+ T Cells in Individuals Receiving Highly Active Antiretroviral Therapy (HAART). J. Immunol. 162: 1780-1788 [Abstract] [Full Text]
Pion, S., Christianson, G. J., Fontaine, P., Roopenian, D. C., Perreault, C. (1999). Shaping the Repertoire of Cytotoxic T-Lymphocyte Responses: Explanation for the Immunodominance Effect Whereby Cytotoxic T Lymphocytes Specific for Immunodominant Antigens Prevent Recognition of Nondominant Antigens. Blood 93: 952-962 [Abstract] [Full Text]
Gugasyan, R., Vidavsky, I., Nelson, C. A., Gross, M. L., Unanue, E. R. (1998). Isolation and Quantitation of a Minor Determinant of Hen Egg White Lysozyme Bound to I-Ak by Using Peptide-Specific Immunoaffinity. J. Immunol. 161: 6074-6083 [Abstract] [Full Text]
Paradela, A., Garcia-Peydro, M., Vazquez, J., Rognan, D., Lopez de Castro, J. A. (1998). The Same Natural Ligand Is Involved in Allorecognition of Multiple HLA-B27 Subtypes by a Single T Cell Clone: Role of Peptide and the MHC Molecule in Alloreactivity. J. Immunol. 161: 5481-5490 [Abstract] [Full Text]
Harrer, T., Harrer, E., Barbosa, P., Kaufmann, F., Wagner, R., Bruggemann, S., Kalden, J. R., Feinberg, M., Johnson, R. P., Buchbinder, S., Walker, B. D. (1998). Recognition of Two Overlapping CTL Epitopes in HIV-1 p17 by CTL from a Long-Term Nonprogressing HIV-1-Infected Individual. J. Immunol. 161: 4875-4881 [Abstract] [Full Text]
Van Baalen, C. A., Schutten, M., Huisman, R. C., Boers, P. H.�M., Gruters, R. A., Osterhaus, A. D.�M.�E. (1998). Kinetics of Antiviral Activity by Human Immunodeficiency Virus Type 1-Specific Cytotoxic T Lymphocytes (CTL) and Rapid Selection of CTL Escape Virus In Vitro. J. Virol. 72: 6851-6857 [Abstract] [Full Text]
Kmieciak, D., Wasik, T. J., Teppler, H., Pientka, J., Hsu, S. H., Takahashi, H., Okumura, K., Kaneko, Y., Kozbor, D. (1998). The Effect of Deletion of the V3 Loop of gp120 on Cytotoxic T Cell Responses and HIV gp120-Mediated Pathogenesis. J. Immunol. 160: 5676-5683 [Abstract] [Full Text]
Gallimore, A., Dumrese, T., Hengartner, H., Zinkernagel, R. M., Rammensee, H.-G. (1998). Protective Immunity Does Not Correlate with the Hierarchy of �Virus-specific Cytotoxic T Cell Responses to Naturally Processed Peptides. JEM 187: 1647-1657 [Abstract] [Full Text]
Durali, D., Morvan, J., Letourneur, F., Schmitt, D., Guegan, N., Dalod, M., Saragosti, S., Sicard, D., Levy, J.-P., Gomard, E. (1998). Cross-Reactions between the Cytotoxic T-Lymphocyte Responses of Human Immunodeficiency Virus-Infected African and European Patients. J. Virol. 72: 3547-3553 [Abstract] [Full Text]
Poon, D. T.�K., Li, G., Aldovini, A. (1998). Nucleocapsid and Matrix Protein Contributions to Selective Human Immunodeficiency Virus Type 1�Genomic RNA Packaging. J. Virol. 72: 1983-1993 [Abstract] [Full Text]
Uger, R. A., Barber, B. H. (1998). Creating CTL Targets with Epitope-Linked {beta}2-Microglobulin Constructs. J. Immunol. 160: 1598-1605 [Abstract] [Full Text]
Nelson, G. W., Kaslow, R., Mann, D. L. (1997). Frequency of HLA allele-specific peptide motifs in HIV-1 proteins correlates with the allele's association with relative rates of disease progression after HIV-1�infection. Proc. Natl. Acad. Sci. USA 94: 9802-9807 [Abstract] [Full Text]
Goulder, P.J.R., Sewell, A.K., Lalloo, D.G., Price, D.A., Whelan, J.A., Evans, J., Taylor, G.P., Luzzi, G., Giangrande, P., Phillips, R.E., McMichael, A.J. (1997). Patterns of Immunodominance in HIV-1-specific Cytotoxic T Lymphocyte Responses in Two Human Histocompatibility Leukocyte Antigens (HLA)-identical Siblings with HLA-A*0201 Are Influenced by Epitope Mutation. JEM 185: 1423-1433 [Abstract] [Full Text]
Stuhler, G., Schlossman, S.�F. (1997). Antigen organization regulates cluster formation and induction of cytotoxic T lymphocytes by helper T cell�subsets. Proc. Natl. Acad. Sci. USA 94: 622-627 [Abstract] [Full Text]
Wang, R., Epstein, J., Baraceros, F. M., Gorak, E. J., Charoenvit, Y., Carucci, D. J., Hedstrom, R. C., Rahardjo, N., Gay, T., Hobart, P., Stout, R., Jones, T. R., Richie, T. L., Parker, S. E., Doolan, D. L., Norman, J., Hoffman, S. L. (2001). Induction of CD4+ T cell-dependent CD8+ type 1 responses in humans by a malaria DNA vaccine. Proc. Natl. Acad. Sci. USA 98: 10817-10822 [Abstract] [Full Text]