Stimulation of CD28 triggers an association between CD28 and phosphatidylinositol 3-kinase in Jurkat T cells

doi:10.1084/jem.179.3.1071

This Article

	Full Text (PDF, 885K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Truitt, K. E.
	Articles by Imboden, J. B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Truitt, K. E.
	Articles by Imboden, J. B.


Social Bookmarking

	What's this?

ARTICLES

Stimulation of CD28 triggers an association between CD28 and phosphatidylinositol 3-kinase in Jurkat T cells

KE Truitt, CM Hicks and JB Imboden
Department of Medicine, San Francisco Veterans Affairs Medical Center, California.

The T cell surface molecule CD28 can provide costimulatory signals that permit the full activation of T cells. Here we demonstrate that stimulation of CD28, either by B7, its natural ligand, or by the anti- CD28 monoclonal antibody 9.3, induces an association between CD28 and phosphatidylinositol 3-kinase (PI3-K) in Jurkat T cells, raising the possibility that an interaction with PI3-K contributes to CD28-mediated signaling. To examine the mechanism of the association, we synthesized tyrosine-phosphorylated oligopeptides corresponding to each of the four tyrosines in the CD28 cytoplasmic domain. When added to lysates of B7- stimulated Jurkat cells, the oligopeptide corresponding to Tyr 173 inhibits the coimmunoprecipitation of PI3-K with CD28; the other oligopeptides have no effect. Tyr 173 is contained within the sequence YMNM, a motif that is also found in the platelet-derived growth factor receptor and that, when phosphorylated, forms a high affinity binding site for the p85 subunit of PI3-K. These observations suggest that phosphorylation of Tyr 173 may mediate the interaction between CD28 and PI3-K. However, because CD28 is not known to be phosphorylated, it remains possible that CD28 interacts with PI3-K through a mechanism independent of tyrosine phosphorylation.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Yarke, C. A., Dalheimer, S. L., Zhang, N., Catron, D. M., Jenkins, M. K., Mueller, D. L. (2008). Proliferating CD4+ T Cells Undergo Immediate Growth Arrest upon Cessation of TCR Signaling In Vivo. J. Immunol. 180: 156-162 [Abstract] [Full Text]
Nirula, A., Ho, M., Phee, H., Roose, J., Weiss, A. (2006). Phosphoinositide-dependent kinase 1 targets protein kinase A in a pathway that regulates interleukin 4. JEM 203: 1733-1744 [Abstract] [Full Text]
Wabnitz, G. H., Nebl, G., Klemke, M., Schroder, A. J., Samstag, Y. (2006). Phosphatidylinositol 3-Kinase Functions as a Ras Effector in the Signaling Cascade That Regulates Dephosphorylation of the Actin-Remodeling Protein Cofilin after Costimulation of Untransformed Human T Lymphocytes. J. Immunol. 176: 1668-1674 [Abstract] [Full Text]
Wu, L. X., La Rose, J., Chen, L., Neale, C., Mak, T., Okkenhaug, K., Wange, R., Rottapel, R. (2005). CD28 Regulates the Translation of Bcl-xL via the Phosphatidylinositol 3-Kinase/Mammalian Target of Rapamycin Pathway. J. Immunol. 174: 180-194 [Abstract] [Full Text]
Sadra, A., Cinek, T., Imboden, J. B. (2004). Translocation of CD28 to lipid rafts and costimulation of IL-2. Proc. Natl. Acad. Sci. USA 101: 11422-11427 [Abstract] [Full Text]
Chemnitz, J. M., Parry, R. V., Nichols, K. E., June, C. H., Riley, J. L. (2004). SHP-1 and SHP-2 Associate with Immunoreceptor Tyrosine-Based Switch Motif of Programmed Death 1 upon Primary Human T Cell Stimulation, but Only Receptor Ligation Prevents T Cell Activation. J. Immunol. 173: 945-954 [Abstract] [Full Text]
Tskvitaria-Fuller, I., Rozelle, A. L., Yin, H. L., Wulfing, C. (2003). Regulation of Sustained Actin Dynamics by the TCR and Costimulation as a Mechanism of Receptor Localization. J. Immunol. 171: 2287-2295 [Abstract] [Full Text]
Matsumoto, A., Seki, Y.-i., Watanabe, R., Hayashi, K., Johnston, J. A., Harada, Y., Abe, R., Yoshimura, A., Kubo, M. (2003). A Role of Suppressor of Cytokine Signaling 3 (SOCS3/CIS3/SSI3) in CD28-mediated Interleukin 2 Production. JEM 197: 425-436 [Abstract] [Full Text]
Ho, E. L., Carayannopoulos, L. N., Poursine-Laurent, J., Kinder, J., Plougastel, B., Smith, H. R. C., Yokoyama, W. M. (2002). Costimulation of Multiple NK Cell Activation Receptors by NKG2D. J. Immunol. 169: 3667-3675 [Abstract] [Full Text]
Venuprasad, K., Banerjee, P. P., Chattopadhyay, S., Sharma, S., Pal, S., Parab, P. B., Mitra, D., Saha, B. (2002). Human Neutrophil-Expressed CD28 Interacts with Macrophage B7 to Induce Phosphatidylinositol 3-Kinase-Dependent IFN-{gamma} Secretion and Restriction of Leishmania Growth. J. Immunol. 169: 920-928 [Abstract] [Full Text]
Cook, J. A., August, A., Henderson, A. J. (2002). Recruitment of Phosphatidylinositol 3-Kinase to CD28 Inhibits HIV Transcription by a Tat-Dependent Mechanism. J. Immunol. 169: 254-260 [Abstract] [Full Text]
Martin, M., Schneider, H., Azouz, A., Rudd, C. E. (2001). Cytotoxic T Lymphocyte Antigen 4 and CD28 Modulate Cell Surface Raft Expression in Their Regulation of T Cell Function. JEM 194: 1675-1682 [Abstract] [Full Text]
Ni, H.-T., Deeths, M. J., Mescher, M. F. (2001). LFA-1-Mediated Costimulation of CD8+ T Cell Proliferation Requires Phosphatidylinositol 3-Kinase Activity. J. Immunol. 166: 6523-6529 [Abstract] [Full Text]
Harada, Y., Tokushima, M., Matsumoto, Y., Ogawa, S., Otsuka, M., Hayashi, K., Weiss, B. D., June, C. H., Abe, R. (2001). Critical Requirement for the Membrane-Proximal Cytosolic Tyrosine Residue for CD28-Mediated Costimulation In Vivo. J. Immunol. 166: 3797-3803 [Abstract] [Full Text]
Yu, C.-T., Shih, H.-m., Lai, M.-Z. (2001). Multiple Signals Required for Cyclic AMP-Responsive Element Binding Protein (CREB) Binding Protein Interaction Induced by CD3/CD28 Costimulation. J. Immunol. 166: 284-292 [Abstract] [Full Text]
Wu, J., Cherwinski, H., Spies, T., Phillips, J. H., Lanier, L. L. (2000). DAP10 and DAP12 Form Distinct, but Functionally Cooperative, Receptor Complexes in Natural Killer Cells. JEM 192: 1059-1068 [Abstract] [Full Text]
Witherden, D. A., Boismenu, R., Havran, W. L. (2000). CD81 and CD28 Costimulate T Cells Through Distinct Pathways. J. Immunol. 165: 1902-1909 [Abstract] [Full Text]
Secchiero, P., Zella, D., Curreli, S., Mirandola, P., Capitani, S., Gallo, R. C., Zauli, G. (2000). Engagement of CD28 Modulates CXC Chemokine Receptor 4 Surface Expression in Both Resting and CD3-Stimulated CD4+ T Cells. J. Immunol. 164: 4018-4024 [Abstract] [Full Text]
Yashiro-Ohtani, Y., Zhou, X.-Y., Toyo-oka, K., Tai, X.-G., Park, C.-S., Hamaoka, T., Abe, R., Miyake, K., Fujiwara, H. (2000). Non-CD28 Costimulatory Molecules Present in T Cell Rafts Induce T Cell Costimulation by Enhancing the Association of TCR with Rafts. J. Immunol. 164: 1251-1259 [Abstract] [Full Text]
Lan, Z., Wu, H., Li, W., Wu, S., Lu, L., Xu, M., Dai, W. (2000). Transforming activity of receptor tyrosine kinase Tyro3 is mediated, at least in part, by the PI3 kinase-signaling pathway. Blood 95: 633-638 [Abstract] [Full Text]
Cuevas, B., Lu, Y., Watt, S., Kumar, R., Zhang, J., Siminovitch, K. A., Mills, G. B. (1999). SHP-1 Regulates Lck-induced Phosphatidylinositol 3-Kinase Phosphorylation and Activity. J. Biol. Chem. 274: 27583-27589 [Abstract] [Full Text]
Leung, B. L., Haughn, L., Veillette, A., Hawley, R. G., Rottapel, R., Julius, M. (1999). TCR{alpha}{beta}-Independent CD28 Signaling and Costimulation Require Non-CD4-Associated Lck. J. Immunol. 163: 1334-1341 [Abstract] [Full Text]
Wu, J., Song, Y., Bakker, A. B., Bauer, S., Spies, T., Lanier, L. L., Phillips, J. H. (1999). An Activating Immunoreceptor Complex Formed by NKG2D and DAP10. Science 285: 730-732 [Abstract] [Full Text]
Borrione, P., Peola, S., Mariani, S., Besostri, B., Mallone, R., Malavasi, F., Pileri, A., Massaia, M. (1999). CD38 Stimulation Lowers the Activation Threshold and Enhances the Alloreactivity of Cord Blood T Cells by Activating the Phosphatidylinositol 3-Kinase Pathway and Inducing CD73 Expression. J. Immunol. 162: 6238-6246 [Abstract] [Full Text]
Ni, H.-T., Deeths, M. J., Li, W., Mueller, D. L., Mescher, M. F. (1999). Signaling Pathways Activated by Leukocyte Function-Associated Ag-1-Dependent Costimulation. J. Immunol. 162: 5183-5189 [Abstract] [Full Text]
Sadra, A., Cinek, T., Arellano, J. L., Shi, J., Truitt, K. E., Imboden, J. B. (1999). Identification of Tyrosine Phosphorylation Sites in the CD28 Cytoplasmic Domain and Their Role in the Costimulation of Jurkat T Cells. J. Immunol. 162: 1966-1973 [Abstract] [Full Text]
Slavik, J. M., Hutchcroft, J. E., Bierer, B. E. (1999). CD80 and CD86 Are Not Equivalent in Their Ability to Induce the Tyrosine Phosphorylation of CD28. J. Biol. Chem. 274: 3116-3124 [Abstract] [Full Text]
Barz, C., Nagel, T., Truitt, K. E., Imboden, J. B. (1998). Mutational Analysis of CD28-Mediated Costimulation of Jun-N-Terminal Kinase and IL-2 Production. J. Immunol. 161: 5366-5372 [Abstract] [Full Text]
Lu, Y., Cuevas, B., Gibson, S., Khan, H., LaPushin, R., Imboden, J., Mills, G. B. (1998). Phosphatidylinositol 3-Kinase Is Required for CD28 But Not CD3 Regulation of the TEC Family Tyrosine Kinase EMT/ITK/TSK: Functional and Physical Interaction of EMT with Phosphatidylinositol 3-Kinase. J. Immunol. 161: 5404-5412 [Abstract] [Full Text]
Marti, F., Xu, C. W., Selvakumar, A., Brent, R., Dupont, B., King, P. D. (1998). LCK-phosphorylated human killer cell-inhibitory receptors recruit and activate phosphatidylinositol 3-kinase. Proc. Natl. Acad. Sci. USA 95: 11810-11815 [Abstract] [Full Text]
Siefken, R., Klein-He{beta}ling, S., Serfling, E., Kurrle, R., Schwinzer, R. (1998). A CD28-Associated Signaling Pathway Leading to Cytokine Gene Transcription and T Cell Proliferation Without TCR Engagement. J. Immunol. 161: 1645-1651 [Abstract] [Full Text]
Lee, D. M., Staats, H. F., Sundy, J. S., Patel, D. D., Sempowski, G. D., Scearce, R. M., Jones, D. M., Haynes, B. F. (1998). Immunologic Characterization of CD7-Deficient Mice. J. Immunol. 160: 5749-5756 [Abstract] [Full Text]
Kaga, S., Ragg, S., Rogers, K. A., Ochi, A. (1998). Activation of p21-CDC42/Rac-Activated Kinases by CD28 Signaling: p21-Activated Kinase (PAK) and MEK Kinase 1 (MEKK1) May Mediate the Interplay Between CD3 and CD28 Signals. J. Immunol. 160: 4182-4189 [Abstract] [Full Text]
Porcu, P., Gaddy, J., Broxmeyer, H. E. (1998). Alloantigen-induced unresponsiveness in cord blood T lymphocytes is associated with defective activation of Ras. Proc. Natl. Acad. Sci. USA 95: 4538-4543 [Abstract] [Full Text]
Cefai, D., Schneider, H., Matangkasombut, O., Kang, H., Brody, J., Rudd, C. E. (1998). CD28 Receptor Endocytosis Is Targeted by Mutations That Disrupt Phosphatidylinositol 3-Kinase Binding and Costimulation. J. Immunol. 160: 2223-2230 [Abstract] [Full Text]
Kim, H.-H., Tharayil, M., Rudd, C. E. (1998). Growth Factor Receptor-bound Protein 2�SH2/SH3 Domain Binding to CD28 and Its Role in Co-signaling. J. Biol. Chem. 273: 296-301 [Abstract] [Full Text]
Punt, J. A., Havran, W., Abe, R., Sarin, A., Singer, A. (1997). T Cell Receptor (TCR)-induced Death of Immature CD4+CD8+ Thymocytes by Two Distinct Mechanisms Differing in Their Requirement for CD28 Costimulation: Implications for Negative Selection in the Thymus. JEM 186: 1911-1922 [Abstract] [Full Text]
Liao, X. C., Fournier, S., Killeen, N., Weiss, A., Allison, J. P., Littman, D. R. (1997). Itk Negatively Regulates Induction of �T Cell Proliferation by CD28 Costimulation. JEM 186: 221-228 [Abstract] [Full Text]
Wu, Y., Guo, Y., Huang, A., Zheng, P., Liu, Y. (1997). CTLA-4-B7 Interaction Is Sufficient to Costimulate T Cell Clonal Expansion. JEM 185: 1327-1336 [Abstract] [Full Text]
Hutchcroft, J. E., Tsai, B., Bierer, B. E. (1996). Differential Phosphorylation of the T Lymphocyte Costimulatory Receptor CD28. ACTIVATION-DEPENDENT CHANGES AND REGULATION BY PROTEIN KINASE C. J. Biol. Chem. 271: 13362-13370 [Abstract] [Full Text]
Pag�s, F.�o., Ragueneau, M., Klasen, S., Battifora, M., Couez, D., Sweet, R., Truneh, A., Ward, S. G., Olive, D. (1996). Two Distinct Intracytoplasmic Regions of the T-cell Adhesion Molecule CD28 Participate in Phosphatidylinositol 3-Kinase Association. J. Biol. Chem. 271: 9403-9409 [Abstract] [Full Text]
Gibson, S., August, A., Branch, D., Dupont, B., Mills, G. B. (1996). Functional LCK Is Required for Optimal CD28-mediated Activation of the TEC Family Tyrosine Kinase EMT/ITK. J. Biol. Chem. 271: 7079-7083 [Abstract] [Full Text]
Leung, H. T., Bradshaw, J., Cleaveland, J. S., Linsley, P. S. (1995). Cytotoxic T Lymphocyte-associated Molecule-4, a High Avidity Receptor for CD80 and CD86, Contains an Intracellular Localization Motif in Its Cytoplasmic Tail. J. Biol. Chem. 270: 25107-25114 [Abstract] [Full Text]
Hartley, D., Meisner, H., Corvera, S. (1995). Specific Association of the beta Isoform of the p85 Subunit of Phosphatidylinositol-3 Kinase with the Proto-oncogene c-cbl. J. Biol. Chem. 270: 18260-18263 [Abstract] [Full Text]
Harada, Y., Tanabe, E., Watanabe, R., Weiss, B. D., Matsumoto, A., Ariga, H., Koiwai, O., Fukui, Y., Kubo, M., June, C. H., Abe, R. (2001). Novel Role of Phosphatidylinositol 3-Kinase in CD28-mediated Costimulation. J. Biol. Chem. 276: 9003-9008 [Abstract] [Full Text]
Kang, H., Schneider, H., Rudd, C. E. (2002). Phosphatidylinositol 3-Kinase p85 Adaptor Function in T-cells. CO-STIMULATION AND REGULATION OF CYTOKINE TRANSCRIPTION INDEPENDENT OF ASSOCIATED p110. J. Biol. Chem. 277: 912-921 [Abstract] [Full Text]