Characterization of the function of intercellular adhesion molecule (ICAM)-3 and comparison with ICAM-1 and ICAM-2 in immune responses

doi:10.1084/jem.179.2.619

This Article

	Full Text (PDF, 1911K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by de Fougerolles, A. R.
	Articles by Springer, T. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by de Fougerolles, A. R.
	Articles by Springer, T. A.


Social Bookmarking

	What's this?

ARTICLES

Characterization of the function of intercellular adhesion molecule (ICAM)-3 and comparison with ICAM-1 and ICAM-2 in immune responses

AR de Fougerolles, X Qin and TA Springer
Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115.

We have characterized the immunobiology of the interaction of intercellular adhesion molecule 3 (ICAM-3; CD50) with its counter- receptor, leukocyte function-associated antigen 1 (LFA-1; CD11a/CD18). Purified ICAM-3 supported LFA-1-dependent adhesion in a temperature- and cation-dependent manner. Activation of cells bearing LFA-1 increased adhesiveness for ICAM-3 in parallel to adhesiveness for ICAM- 1. Although CBR-IC3/1 monoclonal antibody (mAb) blocked adhesion of cells to purified LFA-1, when tested alone, neither CBR-IC3/1 nor five novel ICAM-3 mAbs characterized here blocked adhesion of cells to purified ICAM-3 or homotypic adhesion. Two ICAM-3 mAbs, CBR-IC3/1 and CBR-IC3/2, were required to block LFA-1-dependent adhesion to purified ICAM-3- or LFA-1-dependent, ICAM-1-, ICAM-2-independent homotypic adhesion of lymphoid cell lines. Two ICAM-3 mAbs, CBR-IC3/1 and CBR- IC3/6, induced LFA-1-independent aggregation that was temperature and divalent cation dependent and was completely inhibited by ICAM-3 mAb, CBR-IC3/2, recognizing a distinct epitope. Purified ICAM-3 provided a costimulatory signal for proliferation of resting T lymphocytes. mAb to ICAM-3, together with mAbs to ICAM-1 and ICAM-2, inhibited peripheral blood lymphocyte proliferation in response to phytohemagglutinin, allogeneic stimulator cells, and specific antigen. Inhibition was almost complete and to the same level as with mAb to LFA-1, suggesting the most functionally important, and possibly all, of the ligands for LFA-1 have been defined.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Jolly, C., Mitar, I., Sattentau, Q. J. (2007). Adhesion Molecule Interactions Facilitate Human Immunodeficiency Virus Type 1-Induced Virological Synapse Formation between T Cells. J. Virol. 81: 13916-13921 [Abstract] [Full Text]
Huang, M.-T., Larbi, K. Y., Scheiermann, C., Woodfin, A., Gerwin, N., Haskard, D. O., Nourshargh, S. (2006). ICAM-2 mediates neutrophil transmigration in vivo: evidence for stimulus specificity and a role in PECAM-1-independent transmigration. Blood 107: 4721-4727 [Abstract] [Full Text]
Drukker, M., Katchman, H., Katz, G., Even-Tov Friedman, S., Shezen, E., Hornstein, E., Mandelboim, O., Reisner, Y., Benvenisty, N. (2006). Human Embryonic Stem Cells and Their Differentiated Derivatives Are Less Susceptible to Immune Rejection Than Adult Cells. Stem Cells 24: 221-229 [Abstract] [Full Text]
Song, G., Yang, Y., Liu, J.-h., Casasnovas, J. M., Shimaoka, M., Springer, T. A., Wang, J.-h. (2005). An atomic resolution view of ICAM recognition in a complex between the binding domains of ICAM-3 and integrin {alpha}L{beta}2. Proc. Natl. Acad. Sci. USA 102: 3366-3371 [Abstract] [Full Text]
Crawford, K., Stark, A., Kitchens, B., Sternheim, K., Pantazopoulos, V., Triantafellow, E., Wang, Z., Vasir, B., Larsen, C. E., Gabuzda, D., Reinherz, E., Alper, C. A. (2003). CD2 engagement induces dendritic cell activation: implications for immune surveillance and T-cell activation. Blood 102: 1745-1752 [Abstract] [Full Text]
Tohyama, Y., Katagiri, K., Pardi, R., Lu, C., Springer, T. A., Kinashi, T. (2003). The Critical Cytoplasmic Regions of the {alpha}L/{beta}2 Integrin in Rap1-induced Adhesion and Migration. Mol. Biol. Cell 14: 2570-2582 [Abstract] [Full Text]
Smits, H. H., de Jong, E. C., Schuitemaker, J. H. N., Geijtenbeek, T. B. H., van Kooyk, Y., Kapsenberg, M. L., Wierenga, E. A. (2002). Intercellular Adhesion Molecule-1/LFA-1 Ligation Favors Human Th1 Development. J. Immunol. 168: 1710-1716 [Abstract] [Full Text]
Beals, C. R., Edwards, A. C., Gottschalk, R. J., Kuijpers, T. W., Staunton, D. E. (2001). CD18 Activation Epitopes Induced by Leukocyte Activation. J. Immunol. 167: 6113-6122 [Abstract] [Full Text]
Babina, M., Mammeri, K., Henz, B. M. (2001). Retinoic acid up-regulates myeloid ICAM-3 expression and function in a cell-specific fashion--evidence for retinoid signaling pathways in the mast cell lineage. J. Leukoc. Biol. 69: 361-372 [Abstract] [Full Text]
Bleijs, D., Binnerts, M., van Vliet, S., Figdor, C., van Kooyk, Y (2000). Low-affinity LFA-1/ICAM-3 interactions augment LFA-1/ICAM-1-mediated T cell adhesion and signaling by redistribution of LFA-1. J. Cell Sci. 113: 391-400 [Abstract]
Salomon, B., Bluestone, J. A. (1998). Cutting Edge: LFA-1 Interaction with ICAM-1 and ICAM-2 Regulates Th2 Cytokine Production. J. Immunol. 161: 5138-5142 [Abstract] [Full Text]
Heiska, L., Alfthan, K., Gronholm, M., Vilja, P., Vaheri, A., Carpen, O. (1998). Association of Ezrin with Intercellular Adhesion Molecule-1 and -2�(ICAM-1 and ICAM-2). REGULATION BY PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE. J. Biol. Chem. 273: 21893-21900 [Abstract] [Full Text]
Bell, E. D., May, A. P., Simmons, D. L. (1998). The Leukocyte Function-Associated Antigen-1 (LFA-1)-Binding Site on ICAM-3 Comprises Residues on Both Faces of the First Immunoglobulin Domain. J. Immunol. 161: 1363-1370 [Abstract] [Full Text]
Kessel, J. M., Hayflick, J., Weyrich, A. S., Hoffman, P. A., Gallatin, M., McIntyre, T. M., Prescott, S. M., Zimmerman, G. A. (1998). Coengagement of ICAM-3 and Fc Receptors Induces Chemokine Secretion and Spreading by Myeloid Leukocytes. J. Immunol. 160: 5579-5587 [Abstract] [Full Text]
Schofield, K. P., Humphries, M. J., de Wynter, E., Testa, N., Gallagher, J. T. (1998). The Effect of alpha 4beta 1-Integrin Binding Sequences of Fibronectin on Growth of Cells From Human Hematopoietic Progenitors. Blood 91: 3230-3238 [Abstract] [Full Text]
Woska, J. R. Jr., Morelock, M. M., Jeanfavre, D. D., Caviness, G. O., Bormann, B.-J., Rothlein, R. (1998). Molecular Comparison of Soluble Intercellular Adhesion Molecule (sICAM)-1 and sICAM-3 Binding to Lymphocyte Function-associated Antigen-1. J. Biol. Chem. 273: 4725-4733 [Abstract] [Full Text]
Shanley, T. P., Warner, R. L., Crouch, L. D., Dietsch, G. N., Clark, D. L., O'Brien, M. M., Gallatin, W. M., Ward, P. A. (1998). Requirements for {alpha}d in IgG Immune Complex-Induced Rat Lung Injury. J. Immunol. 160: 1014-1020 [Abstract] [Full Text]
Hallahan, D. E., Virudachalam, S. (1997). Intercellular adhesion molecule 1�knockout abrogates radiation induced pulmonary�inflammation. Proc. Natl. Acad. Sci. USA 94: 6432-6437 [Abstract] [Full Text]
Klickstein, L. B., York, M. R., Fougerolles, A. R.d., Springer, T. A. (1996). Localization of the Binding Site on Intercellular Adhesion Molecule-3 (ICAM-3) for Lymphocyte Function-associated Antigen 1(LFA-1). J. Biol. Chem. 271: 23920-23927 [Abstract] [Full Text]
D'Souza, S. E., Byers-Ward, V. J., Gardiner, E. E., Wang, H., Sung, S.-S. (1996). Identification of an Active Sequence within the First Immunoglobulin Domain of Intercellular Cell Adhesion Molecule-1 (ICAM-1) That Interacts with Fibrinogen. J. Biol. Chem. 271: 24270-24277 [Abstract] [Full Text]
Huang, C., Springer, T. A. (1995). A Binding Interface on the I Domain of Lymphocyte Function-associated Antigen-1 (LFA-1) Required for Specific Interaction with Intercellular Adhesion Molecule 1 (ICAM-1). J. Biol. Chem. 270: 19008-19016 [Abstract] [Full Text]
Clark, E., Brugge, J. (1995). Integrins and signal transduction pathways: the road taken. Science 268: 233-239 [Abstract]
Holness, C. L., Bates, P. A., Littler, A. J., Buckley, C. D., McDowall, A., Bossy, D., Hogg, N., Simmons, D. L. (1995). Analysis of the Binding Site on Intercellular Adhesion Molecule 3 for the Leukocyte Integrin Lymphocyte Function-associated Antigen 1. J. Biol. Chem. 270: 877-884 [Abstract] [Full Text]
Jun, C.-D., Carman, C. V., Redick, S. D., Shimaoka, M., Erickson, H. P., Springer, T. A. (2001). Ultrastructure and Function of Dimeric, Soluble Intercellular Adhesion Molecule-1 (ICAM-1). J. Biol. Chem. 276: 29019-29027 [Abstract] [Full Text]
Yalamanchili, P., Lu, C., Oxvig, C., Springer, T. A. (2000). Folding and Function of I Domain-deleted Mac-1 and Lymphocyte Function-associated Antigen-1. J. Biol. Chem. 275: 21877-21882 [Abstract] [Full Text]