Cytokine mRNA expression during an in vitro response of human B lymphocytes: kinetics of B cell tumor necrosis factor alpha, interleukin (IL)6, IL-10, and transforming growth factor beta 1 mRNAs

doi:10.1084/jem.178.2.521

This Article

	Full Text (PDF, 1308K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Matthes, T.
	Articles by Zubler, R. H.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Matthes, T.
	Articles by Zubler, R. H.


Social Bookmarking

	What's this?

ARTICLES

Cytokine mRNA expression during an in vitro response of human B lymphocytes: kinetics of B cell tumor necrosis factor alpha, interleukin (IL)6, IL-10, and transforming growth factor beta 1 mRNAs

T Matthes, C Werner-Favre, H Tang, X Zhang, V Kindler and RH Zubler
Department of Medicine, Geneva University Hospital, Switzerland.

Expression of mRNA for eight cytokines was analyzed in an in vitro response-proliferation and Ig-secretion--of normal human B lymphocytes. This was made possible by the use of murine thymoma cells as helper cells in conjunction with human T cell supernatant, and the design of human DNA sequence-specific primers for RT-polymerase chain reaction. mRNAs for interleukin (IL)2 and IL-4, but also for IL-1 alpha and IL-1 beta remained undetectable during the whole culture period in highly purified B cells prepared by a three-step purification protocol. However, tumor necrosis factor alpha and IL-6 mRNAs peaked during days 1-3 after culture start and became undetectable after 5-6 d, shortly before bulk B cell proliferation started to decline. In contrast, transforming growth factor beta 1 mRNA, after a progressive increase during the first few days, and IL-10 mRNA, after a peak on days 1-3, remained detectable in immunoglobulin (Ig)-secreting cultures throughout the observation period of 22 d. Clonal analysis on 8-d cultures that had been seeded with single B cells by autocloning with the cell sorter, revealed that 85% of 77 B cell clones studied, expressed TGF-beta 1 mRNA, and only 19% IL-10 mRNA. These findings show a differentiation stage-related cytokine program during a B cell response, whereby (a) B cells can become activated without IL-1 alpha or IL-1 beta expression; (b) mRNA for positive (IL-10) and negative (TGF-beta 1) autoregulatory factors coexists in cell populations during the later phase of the response, although not necessarily in all B cell clones; and (c) normal Ig-secreting cells cease IL-6 expression in contrast to their malignant counterparts, myeloma cells.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Rivera, J., Zaragoza, O., Casadevall, A. (2005). Antibody-Mediated Protection against Cryptococcus neoformans Pulmonary Infection Is Dependent on B Cells. Infect. Immun. 73: 1141-1150 [Abstract] [Full Text]
Shen, H., Whitmire, J. K., Fan, X., Shedlock, D. J., Kaech, S. M., Ahmed, R. (2003). A Specific Role for B Cells in the Generation of CD8 T Cell Memory by Recombinant Listeria monocytogenes. J. Immunol. 170: 1443-1451 [Abstract] [Full Text]
Mouzaki, A., Theodoropoulou, M., Gianakopoulos, I., Vlaha, V., Kyrtsonis, M.-C., Maniatis, A. (2002). Expression patterns of Th1 and Th2 cytokine genes in childhood idiopathic thrombocytopenic purpura (ITP) at presentation and their modulation by intravenous immunoglobulin G (IVIg) treatment: their role in prognosis. Blood 100: 1774-1779 [Abstract] [Full Text]
Jego, G., Bataille, R., Pellat-Deceunynck, C. (2001). Interleukin-6 is a growth factor for nonmalignant human plasmablasts. Blood 97: 1817-1822 [Abstract] [Full Text]
Zan, H., Cerutti, A., Dramitinos, P., Schaffer, A., Casali, P. (1998). CD40 Engagement Triggers Switching to IgA1 and IgA2 in Human B Cells Through Induction of Endogenous TGF-{beta}: Evidence for TGF-{beta} But Not IL-10-Dependent Direct S{micro}->S{alpha} and Sequential S{micro}->S{gamma}, S{gamma}->S{alpha} DNA Recombination. J. Immunol. 161: 5217-5225 [Abstract] [Full Text]
Kienzle, N., Sculley, T. B., Poulsen, L., Buck, M., Cross, S., Raab-Traub, N., Khanna, R. (1998). Identification of a Cytotoxic T-Lymphocyte Response to the Novel BARF0 Protein of Epstein-Barr Virus: a Critical Role for Antigen Expression. J. Virol. 72: 6614-6620 [Abstract] [Full Text]
Cerutti, A., Zan, H., Schaffer, A., Bergsagel, L., Harindranath, N., Max, E. E., Casali, P. (1998). CD40 Ligand and Appropriate Cytokines Induce Switching to IgG, IgA, and IgE and Coordinated Germinal Center and Plasmacytoid Phenotypic Differentiation in a Human Monoclonal IgM+IgD+ B Cell Line. J. Immunol. 160: 2145-2157 [Abstract] [Full Text]
Le, T., Leung, L., Carroll, W. L., Schibler, K. R. (1997). Regulation of Interleukin-10 Gene Expression: Possible Mechanisms Accounting for Its Upregulation and for Maturational Differences in Its Expression by Blood Mononuclear Cells. Blood 89: 4112-4119 [Abstract] [Full Text]