Lysis of ovarian cancer cells by human lymphocytes redirected with a chimeric gene composed of an antibody variable region and the Fc receptor gamma chain

doi:10.1084/jem.178.1.361

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Lysis of ovarian cancer cells by human lymphocytes redirected with a chimeric gene composed of an antibody variable region and the Fc receptor gamma chain

P Hwu, GE Shafer, J Treisman, DG Schindler, G Gross, R Cowherd, SA Rosenberg and Z Eshhar
Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.

To expand the spectrum of recognition of effector lymphocytes and to redirect them towards predefined targets, we have altered the specificity of human tumor-infiltrating lymphocytes (TIL) through stable modification with chimeric receptor genes consisting of single- chain antibody variable regions linked to the gamma subunit common to the immunoglobulin (Ig)G and IgE Fc receptors. Using either hapten or ovarian carcinoma-specific monoclonal antibodies, we constructed chimeric receptor genes and retrovirally introduced them into CD8+ TIL. Redirected TIL specifically lysed trinitrophenyl-labeled Daudi or a human ovarian carcinoma cell line (IGROV-1), and secreted granulocyte/macrophage colony-stimulating factor upon stimulation with the appropriate antigen. This strategy may allow new approaches towards the adoptive immunotherapy of cancer in humans.
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Abiko, Y. (2000). Passive Immunization Against Dental Caries and Periodontal Disease: Development of Recombinant and Human Monoclonal Antibodies. fake Crit Rev Oral Biol Med 11: 140-158 [Abstract] [Full Text]
Nolan, K. F., Yun, C.-O., Akamatsu, Y., Murphy, J. C., Leung, S.-O., Beecham, E. J., Junghans, R. P. (1999). Bypassing Immunization: Optimized Design of ""Designer T Cells"" against Carcinoembryonic Antigen (CEA)-expressing Tumors, and Lack of Suppression by Soluble CEA. Clin. Cancer Res. 5: 3928-3941 [Abstract] [Full Text]
Steele, J. C., Young, S. P., Goodall, J. C., Gallimore, P. H. (1998). Structural Aspects of the Interaction Between Heterogeneic Human Papillomavirus Type 1 E4-Specific T Cell Receptors and the Same Peptide/HLA-DQ8 Complex. J. Immunol. 161: 4745-4752 [Abstract] [Full Text]
Finney, H. M., Lawson, A. D. G., Bebbington, C. R., Weir, A. N. C. (1998). Chimeric Receptors Providing Both Primary and Costimulatory Signaling in T Cells from a Single Gene Product. J. Immunol. 161: 2791-2797 [Abstract] [Full Text]
Fields, R. C., Shimizu, K., Mule, J. J. (1998). Murine dendritic cells pulsed with whole tumor lysates mediate potent antitumor immune responses in vitro and in vivo. Proc. Natl. Acad. Sci. USA 95: 9482-9487 [Abstract] [Full Text]
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Hege, K. M., Cooke, K. S., Finer, M. H., Zsebo, K. M., Roberts, M. R. (1996). Systemic T Cell-independent Tumor Immunity after Transplantation of Universal Receptor-modified Bone Marrow into SCID Mice. JEM 184: 2261-2270 [Abstract] [Full Text]
Kershaw, M. H., Darcy, P. K., Hulett, M. D., Hogarth, P. M., Trapani, J. A., Smyth, M. J. (1996). Redirected Cytotoxic Effector Function. REQUIREMENTS FOR EXPRESSION OF CHIMERIC SINGLE CHAIN HIGH AFFINITY IMMUNOGLOBULIN E RECEPTORS. J. Biol. Chem. 271: 21214-21220 [Abstract] [Full Text]