Two adjacent residues in staphylococcal enterotoxins A and E determine T cell receptor V beta specificity

doi:10.1084/jem.177.1.175

This Article

	Full Text (PDF, 1236K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Hudson, K. R.
	Articles by Fraser, J. D.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Hudson, K. R.
	Articles by Fraser, J. D.


Social Bookmarking

	What's this?

ARTICLES

Two adjacent residues in staphylococcal enterotoxins A and E determine T cell receptor V beta specificity

KR Hudson, H Robinson and JD Fraser
Department of Molecular Medicine, University of Auckland, New Zealand.

The T cell receptor (TCR) V beta-determining region of two bacterial superantigens, staphylococcal enterotoxin A (SEA) and SEE, has been mapped to the COOH-terminal region of SEA and SEE using a panel of recombinant SEA/SEE hybrids. Total TCR V beta mRNA enrichment in human peripheral blood T cell cultures was determined by a novel single-tube amplification technique using a redundant V beta-specific primer. SEA routinely enriched mRNA coding for hV beta 1.1, 5.3, 6.3, 6.4, 6.9, 7.3, 7.4, and 9.1, while SEE, which is 83% homologous to SEA, enriched hV beta 5.1, 6.3, 6.4, 6.9, and 8.1 mRNA. Exchanging residues 206 and 207 was sufficient to convert in toto the TCR V beta response of human peripheral T lymphocytes. In addition, an SEA-reactive murine T cell line, SO3 (mV beta 17), unresponsive to wild-type SEE responded to SEE- S206N207, while an SEE-specific human T cell line, Jurkat (hV beta 8.1), unresponsive to SEA was stimulated strongly by SEA-P206D207. Exchanging all other regions of SEA and SEE except residues 206 and 207 did little to change the V beta response. Thus, the V beta binding region appears to be a stable, discrete domain localized within the COOH-terminal region that is largely unaffected by the considerable amino acid variability between SEA and SEE. This region may interact directly with TCR V beta.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Ramsland, P. A., Willoughby, N., Trist, H. M., Farrugia, W., Hogarth, P. M., Fraser, J. D., Wines, B. D. (2007). Structural basis for evasion of IgA immunity by Staphylococcus aureus revealed in the complex of SSL7 with Fc of human IgA1. Proc. Natl. Acad. Sci. USA 104: 15051-15056 [Abstract] [Full Text]
Omoe, K., Imanishi, K., Hu, D.-L., Kato, H., Fugane, Y., Abe, Y., Hamaoka, S., Watanabe, Y., Nakane, A., Uchiyama, T., Shinagawa, K. (2005). Characterization of Novel Staphylococcal Enterotoxin-Like Toxin Type P. Infect. Immun. 73: 5540-5546 [Abstract] [Full Text]
Llewelyn, M., Sriskandan, S., Peakman, M., Ambrozak, D. R., Douek, D. C., Kwok, W. W., Cohen, J., Altmann, D. M. (2004). HLA Class II Polymorphisms Determine Responses to Bacterial Superantigens. J. Immunol. 172: 1719-1726 [Abstract] [Full Text]
Schafer, P. H., Gandhi, A. K., Loveland, M. A., Chen, R. S., Man, H.-W., Schnetkamp, P. P. M., Wolbring, G., Govinda, S., Corral, L. G., Payvandi, F., Muller, G. W., Stirling, D. I. (2003). Enhancement of Cytokine Production and AP-1 Transcriptional Activity in T Cells by Thalidomide-Related Immunomodulatory Drugs. J. Pharmacol. Exp. Ther. 305: 1222-1232 [Abstract] [Full Text]
Proft, T., Webb, P. D., Handley, V., Fraser, J. D. (2003). Two Novel Superantigens Found in Both Group A and Group C Streptococcus. Infect. Immun. 71: 1361-1369 [Abstract] [Full Text]
Proft, T., Arcus, V. L., Handley, V., Baker, E. N., Fraser, J. D. (2001). Immunological and Biochemical Characterization of Streptococcal Pyrogenic Exotoxins I and J (SPE-I and SPE-J) from Streptococcus pyogenes. J. Immunol. 166: 6711-6719 [Abstract] [Full Text]
Proft, T., Moffatt, S. L., Weller, K. D., Paterson, A., Martin, D., Fraser, J. D. (2000). The Streptococcal Superantigen SMEZ Exhibits Wide Allelic Variation, Mosaic Structure, and Significant Antigenic Variation. JEM 191: 1765-1776 [Abstract] [Full Text]
Proft, T., Louise Moffatt, S., Berkahn, C. J., Fraser, J. D. (1999). Identification and Characterization of Novel Superantigens from Streptococcus pyogenes. JEM 189: 89-102 [Abstract] [Full Text]
Garderet, L., Dulphy, N., Douay, C., Chalumeau, N., Schaeffer, V., Zilber, M.-T., Lim, A., Even, J., Mooney, N., Gelin, C., Gluckman, E., Charron, D., Toubert, A. (1998). The Umbilical Cord Blood alpha beta T-Cell Repertoire: Characteristics of a Polyclonal and Naive but Completely Formed Repertoire. Blood 91: 340-346 [Abstract] [Full Text]
Li, P.-L., Tiedemann, R. E., Moffat, S. L., Fraser, J. D. (1997). The Superantigen Streptococcal Pyrogenic Exotoxin C (SPE-C) Exhibits a Novel Mode of Action. JEM 186: 375-383 [Abstract] [Full Text]