High levels of CD44 expression distinguish virgin from antigen-primed B cells

doi:10.1084/jem.173.3.763

This Article

	Full Text (PDF, 436K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Camp, R. L.
	Articles by Pure, E.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Camp, R. L.
	Articles by Pure, E.


Social Bookmarking

	What's this?

ARTICLES

High levels of CD44 expression distinguish virgin from antigen-primed B cells

RL Camp, TA Kraus, ML Birkeland and E Pure
Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, New York 10021.

The in vitro polyclonal stimulation of B cells through their surface immunoglobulin (Ig) induces substantial increases in CD44 protein levels within 24 hours, whereas other stimuli (e.g., lipopolysaccharide, phorbol 12,13 dibutyrate, and interleukin 4) fail to significantly upregulate CD44. The marked increase in CD44 protein expression on anti-Ig-treated B lymphocytes correlates with an increase in CD44-specific mRNA. Cell sorting experiments with B cells isolated from trinitrophenyl-keyhole limpet hemocyanin-immunized mice demonstrate that both short-term antigen-specific, IgG-secreting cells and long-term antigen-primed B cells are exclusively CD44high. We speculate that the rapid and sustained increase in CD44 expression mediated by surface Ig stimulation may alter the homing properties of antigen-primed B cells.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Hogerkorp, C.-M., Bilke, S., Breslin, T., Ingvarsson, S., Borrebaeck, C. A. K. (2003). CD44-stimulated human B cells express transcripts specifically involved in immunomodulation and inflammation as analyzed by DNA microarrays. Blood 101: 2307-2313 [Abstract] [Full Text]
Mandik-Nayak, L., Huang, G., Sheehan, K. C. F., Erikson, J., Chaplin, D. D. (2001). Signaling Through TNF Receptor p55 in TNF-{alpha}-Deficient Mice Alters the CXCL13/CCL19/CCL21 Ratio in the Spleen and Induces Maturation and Migration of Anergic B Cells into the B Cell Follicle. J. Immunol. 167: 1920-1928 [Abstract] [Full Text]
Wang, H., Shlomchik, M. J. (1999). Autoantigen-specific B Cell Activation in Fas-deficient Rheumatoid Factor Immunoglobulin Transgenic Mice. JEM 190: 639-650 [Abstract] [Full Text]
Ingvarsson, S., Dahlenborg, K., Carlsson, R., Borrebaeck, C. A. K. (1999). Co-ligation of CD44 on naive human tonsillar B cells induces progression towards a germinal center phenotype. Int Immunol 11: 739-744 [Abstract] [Full Text]
Noorchashm, H., Bui, A., Li, H.-L., Eaton, A., Mandik-Nayak, L., Sokol, C., Potts, K. M., Pure, E., Erikson, J. (1999). Characterization of anergic anti-DNA B cells: B cell anergy is a T cell-independent and potentially reversible process. Int Immunol 11: 765-776 [Abstract] [Full Text]
Rachmilewitz, J., Tykocinski, M. L. (1998). Differential Effects of Chondroitin Sulfates A and B on Monocyte and B-Cell Activation: Evidence for B-Cell Activation Via a CD44-Dependent Pathway. Blood 92: 223-229 [Abstract] [Full Text]
Yakushijin, Y., Steckel, J., Kharbanda, S., Hasserjian, R., Neuberg, D., Jiang, W.-m., Anderson, I., Shipp, M. A. (1998). A Directly Spliced Exon 10-Containing CD44 Variant Promotes the Metastasis and Homotypic Aggregation of Aggressive Non-Hodgkin's Lymphoma. Blood 91: 4282-4291 [Abstract] [Full Text]
Pasare, C., Morafo, V., Entringer, M., Bansal, P., George, A., Bal, V., Rath, S., Durdik, J. M. (1998). Presence of Activated Antigen-Binding B Cells During Immunization Enhances Relative Levels of IFN-{gamma} in T Cell Responses. J. Immunol. 160: 778-787 [Abstract] [Full Text]
Mandik-Nayak, L., Bui, A., Noorchashm, H., Eaton, A., Erikson, J. (1997). Regulation of Anti-double-stranded DNA B Cells in Nonautoimmune Mice: Localization to the T-B Interface of the Splenic Follicle. JEM 186: 1257-1267 [Abstract] [Full Text]
Schmits, R., Filmus, J., Gerwin, N., Senaldi, G., Kiefer, F., Kundig, T., Wakeham, A., Shahinian, A., Catzavelos, C., Rak, J., Furlonger, C., Zakarian, A., Simard, J. J.L., Ohashi, P. S., Paige, C. J., Gutierrez-Ramos, J. C., Mak, T. W. (1997). CD44 Regulates Hematopoietic Progenitor Distribution, Granuloma Formation, and Tumorigenicity. Blood 90: 2217-2233 [Abstract] [Full Text]