Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription

doi:10.1084/jem.173.3.711

BioLegend: PerCP, PerCP/Cy5.5 Ab Conjugates

This Article

	Full Text (PDF, 1499K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Schlissel, M. S.
	Articles by Baltimore, D.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Schlissel, M. S.
	Articles by Baltimore, D.


Social Bookmarking

	What's this?

ARTICLES

Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription

MS Schlissel, LM Corcoran and D Baltimore
Whitehead Institute, Cambridge, Massachusetts 02142.

Virus-transformed pre-B cells undergo ordered immunoglobulin (Ig) gene rearrangements during culture. We devised a series of highly sensitive polymerase chain reaction assays for Ig gene rearrangement and unrearranged Ig gene segment transcription to study both the possible relationship between these processes in cultured pre-B cells and the role played by heavy (H) chain (mu) protein in regulating gene rearrangement. Our analysis of pre-B cell cultures representing various stages of maturity revealed that transcription of each germline Ig locus precedes or is coincident with its rearrangement. Cell lines containing one functional rearranged H chain allele, however, continue to transcribe and to rearrange the allelic, unrearranged H chain locus. These cell lines appear to initiate but not terminate rearrangement events and therefore provide information about the requirements for activating rearrangement but not about allelic exclusion mechanisms.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Thomas, L. R., Miyashita, H., Cobb, R. M., Pierce, S., Tachibana, M., Hobeika, E., Reth, M., Shinkai, Y., Oltz, E. M. (2008). Functional Analysis of Histone Methyltransferase G9a in B and T Lymphocytes. J. Immunol. 181: 485-493 [Abstract] [Full Text]
Kiefer, K., Nakajima, P. B., Oshinsky, J., Seeholzer, S. H., Radic, M., Bosma, G. C., Bosma, M. J. (2008). Antigen Receptor Editing in Anti-DNA Transitional B Cells Deficient for Surface IgM. J. Immunol. 180: 6094-6106 [Abstract] [Full Text]
Bhalla, S., Spaulding, C., Brumbaugh, R. L., Zagort, D. E., Massari, M. E., Murre, C., Kee, B. L. (2008). Differential Roles for the E2A Activation Domains in B Lymphocytes and Macrophages. J. Immunol. 180: 1694-1703 [Abstract] [Full Text]
Ju, Z., Volpi, S. A., Hassan, R., Martinez, N., Giannini, S. L., Gold, T., Birshtein, B. K. (2007). Evidence for Physical Interaction between the Immunoglobulin Heavy Chain Variable Region and the 3' Regulatory Region. J. Biol. Chem. 282: 35169-35178 [Abstract] [Full Text]
Shen, L., Zhang, C., Wang, T., Brooks, S., Ford, R. J., Lin-Lee, Y. C., Kasianowicz, A., Kumar, V., Martin, L., Liang, P., Cowell, J., Ambrus, J. L. Jr (2006). Development of Autoimmunity in IL-14{alpha}-Transgenic Mice. J. Immunol. 177: 5676-5686 [Abstract] [Full Text]
Guloglu, F. B., Roman, C. A. J. (2006). Precursor B Cell Receptor Signaling Activity Can Be Uncoupled from Surface Expression.. J. Immunol. 176: 6862-6872 [Abstract] [Full Text]
Afshar, R., Pierce, S., Bolland, D. J., Corcoran, A., Oltz, E. M. (2006). Regulation of IgH Gene Assembly: Role of the Intronic Enhancer and 5'DQ52 Region in Targeting DHJH Recombination. J. Immunol. 176: 2439-2447 [Abstract] [Full Text]
Tarakanova, V. L., Suarez, F., Tibbetts, S. A., Jacoby, M. A., Weck, K. E., Hess, J. L., Speck, S. H., Virgin, H. W. IV (2005). Murine Gammaherpesvirus 68 Infection Is Associated with Lymphoproliferative Disease and Lymphoma in BALB {beta}2 Microglobulin-Deficient Mice. J. Virol. 79: 14668-14679 [Abstract] [Full Text]
Lai, A. Y., Lin, S. M., Kondo, M. (2005). Heterogeneity of Flt3-Expressing Multipotent Progenitors in Mouse Bone Marrow. J. Immunol. 175: 5016-5023 [Abstract] [Full Text]
Pelayo, R., Hirose, J., Huang, J., Garrett, K. P., Delogu, A., Busslinger, M., Kincade, P. W. (2005). Derivation of 2 categories of plasmacytoid dendritic cells in murine bone marrow. Blood 105: 4407-4415 [Abstract] [Full Text]
Hayashi, K., Nojima, T., Goitsuka, R., Kitamura, D. (2004). Impaired Receptor Editing in the Primary B Cell Repertoire of BASH-Deficient Mice. J. Immunol. 173: 5980-5988 [Abstract] [Full Text]
Fuentes-Panana, E. M., Bannish, G., Shah, N., Monroe, J. G. (2004). Basal Ig{alpha}/Ig{beta} Signals Trigger the Coordinated Initiation of Pre-B Cell Antigen Receptor-Dependent Processes. J. Immunol. 173: 1000-1011 [Abstract] [Full Text]
Seet, C. S., Brumbaugh, R. L., Kee, B. L. (2004). Early B Cell Factor Promotes B Lymphopoiesis with Reduced Interleukin 7 Responsiveness in the Absence of E2A. JEM 199: 1689-1700 [Abstract] [Full Text]
Swanson, P. J., Kuslak, S. L., Fang, W., Tze, L., Gaffney, P., Selby, S., Hippen, K. L., Nunez, G., Sidman, C. L., Behrens, T. W. (2004). Fatal Acute Lymphoblastic Leukemia in Mice Transgenic for B Cell-Restricted bcl-xL and c-myc. J. Immunol. 172: 6684-6691 [Abstract] [Full Text]
Hsu, L.-Y., Liang, H.-E., Johnson, K., Kang, C., Schlissel, M. S. (2004). Pax5 Activates Immunoglobulin Heavy Chain V to DJ Rearrangement in Transgenic Thymocytes. JEM 199: 825-830 [Abstract] [Full Text]
Fuxa, M., Skok, J., Souabni, A., Salvagiotto, G., Roldan, E., Busslinger, M. (2004). Pax5 induces V-to-DJ rearrangements and locus contraction of the immunoglobulin heavy-chain gene. Genes Dev. 18: 411-422 [Abstract] [Full Text]
Toyama-Sorimachi, N., Tsujimura, Y., Maruya, M., Onoda, A., Kubota, T., Koyasu, S., Inaba, K., Karasuyama, H. (2004). Ly49Q, a member of the Ly49 family that is selectively expressed on myeloid lineage cells and involved in regulation of cytoskeletal architecture. Proc. Natl. Acad. Sci. USA 101: 1016-1021 [Abstract] [Full Text]
Schweitzer, B. L., DeKoter, R. P. (2004). Analysis of Gene Expression and Ig Transcription in PU.1/Spi-B-Deficient Progenitor B Cell Lines. J. Immunol. 172: 144-154 [Abstract] [Full Text]
Tretter, T., Ross, A. E., Dordai, D. I., Desiderio, S. (2003). Mimicry of Pre-B Cell Receptor Signaling by Activation of the Tyrosine Kinase Blk. JEM 198: 1863-1873 [Abstract] [Full Text]
Sitnicka, E., Brakebusch, C., Martensson, I.-L., Svensson, M., Agace, W. W., Sigvardsson, M., Buza-Vidas, N., Bryder, D., M.Cilio, C., Ahlenius, H., Maraskovsky, E., Peschon, J. J., Jacobsen, S. E. W. (2003). Complementary Signaling through flt3 and Interleukin-7 Receptor {alpha} Is Indispensable for Fetal and Adult B Cell Genesis. JEM 198: 1495-1506 [Abstract] [Full Text]
Dudley, D. D., Sekiguchi, J., Zhu, C., Sadofsky, M. J., Whitlow, S., DeVido, J., Monroe, R. J., Bassing, C. H., Alt, F. W. (2003). Impaired V(D)J Recombination and Lymphocyte Development in Core RAG1-expressing Mice. JEM 198: 1439-1450 [Abstract] [Full Text]
Morvan, C. L., Pinaud, E., Decourt, C., Cuvillier, A., Cogne, M. (2003). The immunoglobulin heavy-chain locus hs3b and hs4 3' enhancers are dispensable for VDJ assembly and somatic hypermutation. Blood 102: 1421-1427 [Abstract] [Full Text]
Tsapogas, P., Breslin, T., Bilke, S., Lagergren, A., Mansson, R., Liberg, D., Peterson, C., Sigvardsson, M. (2003). RNA analysis of B cell lines arrested at defined stages of differentiation allows for an approximation of gene expression patterns during B cell development. J. Leukoc. Biol. 74: 102-110 [Abstract] [Full Text]
Corcoran, L., Ferrero, I., Vremec, D., Lucas, K., Waithman, J., O'Keeffe, M., Wu, L., Wilson, A., Shortman, K. (2003). The Lymphoid Past of Mouse Plasmacytoid Cells and Thymic Dendritic Cells. J. Immunol. 170: 4926-4932 [Abstract] [Full Text]
Oberdoerffer, P., Novobrantseva, T. I., Rajewsky, K. (2003). Expression of a Targeted {lambda}1 Light Chain Gene Is Developmentally Regulated and Independent of Ig{kappa} Rearrangements. JEM 197: 1165-1172 [Abstract] [Full Text]
Johnson, K., Angelin-Duclos, C., Park, S., Calame, K. L. (2003). Changes in Histone Acetylation Are Associated with Differences in Accessibility of VH Gene Segments to V-DJ Recombination during B-Cell Ontogeny and Development. Mol. Cell. Biol. 23: 2438-2450 [Abstract] [Full Text]
Lian, Z.-X., Okada, T., He, X.-S., Kita, H., Liu, Y.-J., Ansari, A. A., Kikuchi, K., Ikehara, S., Gershwin, M. E. (2003). Heterogeneity of Dendritic Cells in the Mouse Liver: Identification and Characterization of Four Distinct Populations. J. Immunol. 170: 2323-2330 [Abstract] [Full Text]
Hesslein, D. G.T., Pflugh, D. L., Chowdhury, D., Bothwell, A. L. M., Sen, R., Schatz, D. G. (2003). Pax5 is required for recombination of transcribed, acetylated, 5' IgH V gene segments. Genes Dev. 17: 37-42 [Abstract] [Full Text]
Delpy, L., Decourt, C., Le Bert, M., Cogne, M. (2002). B Cell Development Arrest Upon Insertion of a neo Gene Between JH and E{micro}: Promoter Competition Results in Transcriptional Silencing of Germline JH and Complete V(D)J Rearrangements. J. Immunol. 169: 6875-6882 [Abstract] [Full Text]
Zhao, F.-Q., Sheng, Z.-M., Tsai, M. M., Hubbs, A. E., Wang, R., O'Leary, T. J., Izon, D. J., Taubenberger, J. K. (2002). Serial analysis of gene expression in murine fetal thymocyte cell lines. Int Immunol 14: 1383-1395 [Abstract] [Full Text]
Sikes, M. L., Meade, A., Tripathi, R., Krangel, M. S., Oltz, E. M. (2002). Regulation of V(D)J recombination: A dominant role for promoter positioning in gene segment accessibility. Proc. Natl. Acad. Sci. USA 99: 12309-12314 [Abstract] [Full Text]
Bender, C. F., Sikes, M. L., Sullivan, R., Huye, L. E., Le Beau, M. M., Roth, D. B., Mirzoeva, O. K., Oltz, E. M., Petrini, J. H. J. (2002). Cancer predisposition and hematopoietic failure in Rad50S/S mice. Genes Dev. 16: 2237-2251 [Abstract] [Full Text]
Tantin, D., Sharp, P. A. (2002). Mouse Lymphoid Cell Line Selected To Have High Immunoglobulin Promoter Activity. Mol. Cell. Biol. 22: 1460-1473 [Abstract] [Full Text]
Herblot, S., Aplan, P. D., Hoang, T. (2002). Gradient of E2A Activity in B-Cell Development. Mol. Cell. Biol. 22: 886-900 [Abstract] [Full Text]
Zou, X., Ayling, C., Xian, J., Piper, T. A., Barker, P. J., Bruggemann, M. (2001). Truncation of the {micro} heavy chain alters BCR signalling and allows recruitment of CD5+ B cells. Int Immunol 13: 1489-1499 [Abstract] [Full Text]
Feeney, A. J., Lawson, B. R., Kono, D. H., Theofilopoulos, A. N. (2001). Terminal Deoxynucleotidyl Transferase Deficiency Decreases Autoimmune Disease in MRL-Faslpr Mice. J. Immunol. 167: 3486-3493 [Abstract] [Full Text]
Goebel, P., Janney, N., Valenzuela, J. R., Romanow, W. J., Murre, C., Feeney, A. J. (2001). Localized Gene-specific Induction of Accessibility to V(D)J Recombination Induced by E2A and Early B Cell Factor in Nonlymphoid Cells. JEM 194: 645-656 [Abstract] [Full Text]
Williams, G. S., Martinez, A., Montalbano, A., Tang, A., Mauhar, A., Ogwaro, K. M., Merz, D., Chevillard, C., Riblet, R., Feeney, A. J. (2001). Unequal VH Gene Rearrangement Frequency Within the Large VH7183 Gene Family Is Not Due to Recombination Signal Sequence Variation, and Mapping of the Genes Shows a Bias of Rearrangement Based on Chromosomal Location. J. Immunol. 167: 257-263 [Abstract] [Full Text]
Lu, L., Bonham, C. A., Liang, X., Chen, Z., Li, W., Wang, L., Watkins, S. C., Nalesnik, M. A., Schlissel, M. S., Demestris, A. J., Fung, J. J., Qian, S. (2001). Liver-Derived DEC205+B220+CD19- Dendritic Cells Regulate T Cell Responses. J. Immunol. 166: 7042-7052 [Abstract] [Full Text]
Mojica, M. P., Perry, S. S., Searles, A. E., Elenitoba-Johnson, K. S. J., Pierce, L. J., Wiesmann, A., Slayton, W. B., Spangrude, G. J. (2001). Phenotypic Distinction and Functional Characterization of Pro-B Cells in Adult Mouse Bone Marrow. J. Immunol. 166: 3042-3051 [Abstract] [Full Text]
Nitschke, L., Kestler, J., Tallone, T., Pelkonen, S., Pelkonen, J. (2001). Deletion of the DQ52 Element Within the Ig Heavy Chain Locus Leads to a Selective Reduction in VDJ Recombination and Altered D Gene Usage. J. Immunol. 166: 2540-2552 [Abstract] [Full Text]
Maki, K., Nagata, K., Kitamura, F., Takemori, T., Karasuyama, H. (2000). Immunoglobulin {beta} Signaling Regulates Locus Accessibility for Ordered Immunoglobulin Gene Rearrangements. JEM 191: 1333-1340 [Abstract] [Full Text]
Medina, K. L., Strasser, A., Kincade, P. W. (2000). Estrogen influences the differentiation, proliferation, and survival of early B-lineage precursors. Blood 95: 2059-2067 [Abstract] [Full Text]
Tachibana, H., Haruta, H., Ueda, K., Chiwata, T., Yamada, K. (2000). Induction of Light Chain Replacement in Human Plasma Cells by Caffeine Is Independent from Both the Upregulation of RAG Protein Expression and Germ Line Transcription. J. Biol. Chem. 275: 5927-5933 [Abstract] [Full Text]
Kuzin, I. I., Ugine, G. D., Wu, D., Young, F., Chen, J., Bottaro, A. (2000). Normal Isotype Switching in B Cells Lacking the I{micro} Exon Splice Donor Site: Evidence for Multiple I{micro}-Like Germline Transcripts. J. Immunol. 164: 1451-1457 [Abstract] [Full Text]
Li, S., Hammer, R. E., George-Raizen, J. B., Meyers, K. C., Garrard, W. T. (2000). High-Level Rearrangement and Transcription of Yeast Artificial Chromosome-Based Mouse Ig{kappa} Transgenes Containing Distal Regions of the Contig. J. Immunol. 164: 812-824 [Abstract] [Full Text]
Morrow, T. A., Muljo, S. A., Zhang, J., Hardwick, J. M., Schlissel, M. S. (1999). Pro-B-Cell-Specific Transcription and Proapoptotic Function of Protein Kinase Ceta. Mol. Cell. Biol. 19: 5608-5618 [Abstract] [Full Text]
Doty, R. T., Xia, D., Nguyen, S. P., Hathaway, T. R., Willerford, D. M. (1999). Promoter Element for Transcription of Unrearranged T-Cell Receptor beta -Chain Gene in Pro-T Cells. Blood 93: 3017-3025 [Abstract] [Full Text]
NIKOLAJCZYK, B.S., DANG, W., SEN, R. (1999). Mechanisms of {micro} Enhancer Regulation in B Lymphocytes. Cold Spring Harb Symp Quant Biol 64: 99-108 [Abstract]
Tachibana, H., Haruta, H., Yamada, K. (1999). Light Chain Shifting: Identification of a Human Plasma Cell Line Actively Undergoing Light Chain Replacement. Blood 93: 198-207 [Abstract] [Full Text]
Angelin-Duclos, C., Calame, K. (1998). Evidence that Immunoglobulin VH-DJ Recombination Does Not Require Germ Line Transcription of the Recombining Variable Gene Segment. Mol. Cell. Biol. 18: 6253-6264 [Abstract] [Full Text]
Cronin, F. E., Jiang, M., Abbas, A. K., Grupp, S. A. (1998). Role of {micro} Heavy Chain in B Cell Development. I. Blocked B Cell Maturation But Complete Allelic Exclusion in the Absence of Ig{alpha}/{beta}. J. Immunol. 161: 252-259 [Abstract] [Full Text]
Malek, S. N., Dordai, D. I., Reim, J., Dintzis, H., Desiderio, S. (1998). Malignant transformation of early lymphoid progenitors in mice expressing an activated Blk tyrosine kinase. Proc. Natl. Acad. Sci. USA 95: 7351-7356 [Abstract] [Full Text]
Ray, R. J., Stoddart, A., Pennycook, J. L., Huner, H. O., Furlonger, C., Wu, G. E., Paige, C. J. (1998). Stromal Cell-Independent Maturation of IL-7-Responsive Pro-B Cells. J. Immunol. 160: 5886-5897 [Abstract] [Full Text]
Zhuang, Y., Barndt, R. J., Pan, L., Kelley, R., Dai, M. (1998). Functional Replacement of the Mouse E2A Gene with a Human HEB cDNA. Mol. Cell. Biol. 18: 3340-3349 [Abstract] [Full Text]
Schlissel, M. S. (1998). Structure of Nonhairpin Coding-End DNA Breaks in Cells Undergoing V(D)J Recombination. Mol. Cell. Biol. 18: 2029-2037 [Abstract] [Full Text]
Constantinescu, A., Schlissel, M. S. (1997). Changes in Locus-specific V(D)J Recombinase Activity Induced by Immunoglobulin Gene Products during B Cell Development. JEM 185: 609-620 [Abstract] [Full Text]
Nutt, S L, Urbanek, P, Rolink, A, Busslinger, M (1997). Essential functions of Pax5 (BSAP) in pro-B cell development: difference between fetal and adult B lymphopoiesis and reduced V-to-DJ recombination at the IgH locus.. Genes Dev. 11: 476-491 [Abstract]
Gong, S., Sanchez, M., Nussenzweig, M. C. (1996). Counterselection against D� Is Mediated through Immunoglobulin (Ig)alpha -Igbeta. JEM 184: 2079-2084 [Abstract] [Full Text]
Tachibana, H., Ushio, Y., Krungkasem, C., Shirahata, S. (1996). Inducing the Loss of Immunoglobulin &lgr;Light Chain Production and the Rearrangement of a Previously Excluded Allele in Human Plasma B Cell Lines with Concanavalin A. J. Biol. Chem. 271: 17404-17410 [Abstract] [Full Text]
Tsai, S, Bartelmez, S, Sitnicka, E, Collins, S (1994). Lymphohematopoietic progenitors immortalized by a retroviral vector harboring a dominant-negative retinoic acid receptor can recapitulate lymphoid, myeloid, and erythroid development.. Genes Dev. 8: 2831-2841 [Abstract]
Scott, E., Simon, M., Anastasi, J, Singh, H (1994). Requirement of transcription factor PU.1 in the development of multiple hematopoietic lineages. Science 265: 1573-1577 [Abstract]
Spanopoulou, E, Roman, C A, Corcoran, L M, Schlissel, M S, Silver, D P, Nemazee, D, Nussenzweig, M C, Shinton, S A, Hardy, R R, Baltimore, D (1994). Functional immunoglobulin transgenes guide ordered B-cell differentiation in Rag-1-deficient mice.. Genes Dev. 8: 1030-1042 [Abstract]
Young, F, Ardman, B, Shinkai, Y, Lansford, R, Blackwell, T K, Mendelsohn, M, Rolink, A, Melchers, F, Alt, F W (1994). Influence of immunoglobulin heavy- and light-chain expression on B-cell differentiation.. Genes Dev. 8: 1043-1057 [Abstract]
Schlissel, M, Constantinescu, A, Morrow, T, Baxter, M, Peng, A (1993). Double-strand signal sequence breaks in V(D)J recombination are blunt, 5'-phosphorylated, RAG-dependent, and cell cycle regulated.. Genes Dev. 7: 2520-2532 [Abstract]
Corcoran, L M, Karvelas, M, Nossal, G J, Ye, Z S, Jacks, T, Baltimore, D (1993). Oct-2, although not required for early B-cell development, is critical for later B-cell maturation and for postnatal survival.. Genes Dev. 7: 570-582 [Abstract]
Schlissel, M, Voronova, A, Baltimore, D (1991). Helix-loop-helix transcription factor E47 activates germ-line immunoglobulin heavy-chain gene transcription and rearrangement in a pre-T-cell line.. Genes Dev. 5: 1367-1376 [Abstract]