Journal of Experimental Medicine, Vol 172, 1451-1457, Copyright © 1990 by Rockefeller University Press

ARTICLES

Formation of lipoxins and leukotrienes during receptor-mediated interactions of human platelets and recombinant human granulocyte/macrophage colony-stimulating factor-primed neutrophils

S Fiore and CN Serhan
Hematology Division, Brigham and Women's Hospital, Boston, Massachusetts 02115.

The generation of lipoxygenase products of arachidonic acid is considered an important event in inflammation. This study demonstrates the levels of both lipoxins and leukotrienes (LTC4, LTD4, LTB4, and omega-oxidized LTB4) generated from endogenous sources of arachidonate by PMN primed with recombinant human granulocyte/macrophage colony- stimulating factor and in coincubations with platelets (1:1 to 1:100 ratio). Upon exposure to receptor-mediated stimuli (FMLP and thrombin), the levels of lipoxins generated were within the range of both LTB4 and LTC4. Co-incubation of [1-14C]arachidonate-labeled platelets with primed polymorphonuclear leukocytes (PMN) followed by addition of thrombin and FMLP led to the formation of both 5- and 15-LO products that carried 14C label. Thus, in addition to the transcellular conversion of LTA4 to platelet-derived lipoxins and LTC4, PMN can use platelet-derived arachidonate to generate lipoxygenase products. These results are the first to document the relationship between the levels of lipoxins and leukotrienes generated by receptor-mediated activation of cytokineprimed PMN interacting with platelets. Moreover, they indicate that PMN-platelet interactions utilize bidirectional transcellular routes to contribute to lipoxin formation.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Starosta, V., Pazdrak, K., Boldogh, I., Svider, T., Kurosky, A. (2008). Lipoxin A4 Counterregulates GM-CSF Signaling in Eosinophilic Granulocytes. J. Immunol. 181: 8688-8699 [Abstract] [Full Text]  
• Planaguma, A., Kazani, S., Marigowda, G., Haworth, O., Mariani, T. J., Israel, E., Bleecker, E. R., Curran-Everett, D., Erzurum, S. C., Calhoun, W. J., Castro, M., Chung, K. F., Gaston, B., Jarjour, N. N., Busse, W. W., Wenzel, S. E., Levy, B. D., for the NHLBI Severe Asthma Research Program (SARP, (2008). Airway Lipoxin A4 Generation and Lipoxin A4 Receptor Expression Are Decreased in Severe Asthma. Am. J. Respir. Crit. Care Med. 178: 574-582 [Abstract] [Full Text]  
• Haworth, O., Levy, B. D. (2007). Endogenous lipid mediators in the resolution of airway inflammation. Eur Respir J 30: 980-992 [Abstract] [Full Text]  
• Rajakariar, R., Yaqoob, M. M., Gilroy, D. W. (2006). COX-2 in Inflammation and Resolution. Mol. Interv. 6: 199-207 [Abstract] [Full Text]  
• Abedin, M., Tintut, Y., Demer, L. L. (2004). Vascular Calcification: Mechanisms and Clinical Ramifications. Arterioscler. Thromb. Vasc. Bio. 24: 1161-1170 [Abstract] [Full Text]  
• Gangemi, S., Luciotti, G., D'Urbano, E., Mallamace, A., Santoro, D., Bellinghieri, G., Davi, G., Romano, M. (2003). Physical exercise increases urinary excretion of lipoxin A4 and related compounds. J. Appl. Physiol. 94: 2237-2240 [Abstract] [Full Text]  
• Burkert, E., Szellas, D., Radmark, O., Steinhilber, D., Werz, O. (2003). Cell type-dependent activation of 5-lipoxygenase by arachidonic acid. J. Leukoc. Biol. 73: 191-200 [Abstract] [Full Text]  
• Schottelius, A. J., Giesen, C., Asadullah, K., Fierro, I. M., Colgan, S. P., Bauman, J., Guilford, W., Perez, H. D., Parkinson, J. F. (2002). An Aspirin-Triggered Lipoxin A4 Stable Analog Displays a Unique Topical Anti-Inflammatory Profile. J. Immunol. 169: 7063-7070 [Abstract] [Full Text]  
• Werz, O., Burkert, E., Samuelsson, B., Radmark, O., Steinhilber, D. (2002). Activation of 5-lipoxygenase by cell stress is calcium independent in human polymorphonuclear leukocytes. Blood 99: 1044-1052 [Abstract] [Full Text]  
• Coffey, M. J., Phare, S. M., Peters-Golden, M. (2000). Prolonged Exposure to Lipopolysaccharide Inhibits Macrophage 5-Lipoxygenase Metabolism Via Induction of Nitric Oxide Synthesis. J. Immunol. 165: 3592-3598 [Abstract] [Full Text]  
• Chavis, C, Vachier, I, Godard, P, Bousquet, J, Chanez, P (2000). Lipoxins and other arachidonate derived mediators in bronchial asthma. Thorax 55: 38S-41 [Full Text]  
• Powell, W. S., Gravel, S., Khanapure, S. P., Rokach, J. (1999). Biological Inactivation of 5-oxo-6,8,11,14-Eicosatetraenoic Acid by Human Platelets. Blood 93: 1086-1096 [Abstract] [Full Text]  
• Surette, M. E., Dallaire, N., Jean, N., Picard, S., Borgeat, P. (1998). Mechanisms of the priming effect of lipopolysaccharides on the biosynthesis of leukotriene B4 in chemotactic peptide-stimulated human neutrophils. FASEB J. 12: 1521-1531 [Abstract] [Full Text]  
• Datta, Y. H., Romano, M., Jacobson, B. C., Golan, D. E., Serhan, C. N., Ewenstein, B. M. (1995). Peptido-Leukotrienes Are Potent Agonists of von Willebrand Factor Secretion and P-Selectin Surface Expression in Human Umbilical Vein Endothelial Cells. Circulation 92: 3304-3311 [Abstract] [Full Text]  
• Lopez-Farre, A., Caramelo, C., Esteban, A., Alberola, M. L., Millas, I., Monton, M., Casado, S. (1995). Effects of Aspirin on Platelet-Neutrophil Interactions : Role of Nitric Oxide and Endothelin-1. Circulation 91: 2080-2088 [Abstract] [Full Text]  
• Ware, J. A., Heistad, D. D. (1993). Platelet-Endothelium Interactions. NEJM 328: 628-635 [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS