Coclustering CD45 with CD4 or CD8 alters the phosphorylation and kinase activity of p56lck

doi:10.1084/jem.172.1.347

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Coclustering CD45 with CD4 or CD8 alters the phosphorylation and kinase activity of p56lck

HL Ostergaard and IS Trowbridge
Department of Cancer Biology, Salk Institute, San Diego, California 92138.

Antibody-mediated CD4 crosslinking results in increased tyrosine phosphorylation and tyrosine kinase activity of the associated p56lck. Treatment with anti-CD4 and anti-Ig also induced the phosphorylation of p56lck in a CD45- mutant cell line, indicating that the increase in phosphotyrosine content of p56lck is not the result of being sequestered from CD45 protein tyrosine phosphatase (PTPase). Antibody- mediated coclustering of CD45 with CD4 inhibited the anti-CD4-induced phosphorylation of p56lck on tyrosine and the concomitant increase in in vitro kinase activity. Similar results were obtained when CD45 was coclustered with CD8 on cytotoxic T cell lines. These observations provide strong evidence that p56lck is a substrate for CD45 in vivo and provide an assay to study the regulation and specificity of CD45 PTPase activity.
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Takehisa, Y., Sakiyama, S., Uyama, T., Sumitomo, M., Tamaki, M., Hino, H., Takehisa, M., Liu, M., Kondo, K., Monden, Y. (2002). Progressive increase of CD4+/CD45RC- lymphocytes after allograft rat lung transplantation: A marker of acute rejection. J. Thorac. Cardiovasc. Surg. 124: 675-683 [Abstract] [Full Text]
Ishikawa, H., Tsuyama, N., Abroun, S., Liu, S., Li, F.-J., Taniguchi, O., Kawano, M. M. (2002). Requirements of src family kinase activity associated with CD45 for myeloma cell proliferation by interleukin-6. Blood 99: 2172-2178 [Abstract] [Full Text]
Blank, N., Kriegel, M., Hieronymus, T., Geiler, T., Winkler, S., Kalden, J. R., Lorenz, H.-M. (2001). CD45 Tyrosine Phosphatase Controls Common {{gamma}}-Chain Cytokine-Mediated STAT and Extracellular Signal-Related Kinase Phosphorylation in Activated Human Lymphoblasts: Inhibition of Proliferation Without Induction of Apoptosis. J. Immunol. 166: 6034-6040 [Abstract] [Full Text]
Chiang, G. G., Sefton, B. M. (2000). Phosphorylation of a Src Kinase at the Autophosphorylation Site in the Absence of Src Kinase Activity. J. Biol. Chem. 275: 6055-6058 [Abstract] [Full Text]
Lynch, K. W., Weiss, A. (2000). A Model System for Activation-Induced Alternative Splicing of CD45 Pre-mRNA in T Cells Implicates Protein Kinase C and Ras. Mol. Cell. Biol. 20: 70-80 [Abstract] [Full Text]
Holdorf, A. D., Green, J. M., Levin, S. D., Denny, M. F., Straus, D. B., Link, V., Changelian, P. S., Allen, P. M., Shaw, A. S. (1999). Proline Residues in CD28 and the Src Homology (SH)3 Domain of Lck Are Required for T Cell Costimulation. JEM 190: 375-384 [Abstract] [Full Text]
Katagiri, T., Ogimoto, M., Hasegawa, K., Arimura, Y., Mitomo, K., Okada, M., Clark, M. R., Mizuno, K., Yakura, H. (1999). CD45 Negatively Regulates Lyn Activity by Dephosphorylating Both Positive and Negative Regulatory Tyrosine Residues in Immature B Cells. J. Immunol. 163: 1321-1326 [Abstract] [Full Text]
Seavitt, J. R., White, L. S., Murphy, K. M., Loh, D. Y., Perlmutter, R. M., Thomas, M. L. (1999). Expression of the p56lck Y505F Mutation in CD45-Deficient Mice Rescues Thymocyte Development. Mol. Cell. Biol. 19: 4200-4208 [Abstract] [Full Text]
D'Oro, U., Ashwell, J. D. (1999). Cutting Edge: The CD45 Tyrosine Phosphatase Is an Inhibitor of Lck Activity in Thymocytes. J. Immunol. 162: 1879-1883 [Abstract] [Full Text]
Corey, S. J., Anderson, S. M. (1999). Src-Related Protein Tyrosine Kinases in Hematopoiesis. Blood 93: 1-14 [Full Text]
Okamoto, S., Azhipa, O., Yu, Y., Russo, E., Dennert, G. (1998). Expression of ADP-Ribosyltransferase on Normal T Lymphocytes and Effects of Nicotinamide Adenine Dinucleotide on Their Function. J. Immunol. 160: 4190-4198 [Abstract] [Full Text]
Duplay, P., Alcover, A., Fargeas, C., Sekaly, R. P., Branton, P. E. (1996). An Activated Epidermal Growth Factor Receptor/Lck Chimera Restores Early T Cell Receptor-mediated Calcium Response in a CD45-deficient T Cell Line. J. Biol. Chem. 271: 17896-17902 [Abstract] [Full Text]
Katagiri, T., Ogimoto, M., Hasegawa, K., Mizuno, K., Yakura, H. (1995). Selective Regulation of Lyn Tyrosine Kinase by CD45 in Immature B Cells. J. Biol. Chem. 270: 27987-27990 [Abstract] [Full Text]
Ostergaard, H., Trowbridge, I. (1991). Negative regulation of CD45 protein tyrosine phosphatase activity by ionomycin in T cells. Science 253: 1423-1425 [Abstract]
Fischer, E., Charbonneau, H, Tonks, N. (1991). Protein tyrosine phosphatases: a diverse family of intracellular and transmembrane enzymes. Science 253: 401-406 [Abstract]
Justement, L., Campbell, K., Chien, N., Cambier, J. (1991). Regulation of B cell antigen receptor signal transduction and phosphorylation by CD45. Science 252: 1839-1842 [Abstract]