Effects of the neutrophil-activating peptide NAP-2, platelet basic protein, connective tissue-activating peptide III and platelet factor 4 on human neutrophils

doi:10.1084/jem.170.5.1745

This Article

	Full Text (PDF, 338K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Walz, A.
	Articles by Baggiolini, M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Walz, A.
	Articles by Baggiolini, M.


Social Bookmarking

	What's this?

ARTICLES

Effects of the neutrophil-activating peptide NAP-2, platelet basic protein, connective tissue-activating peptide III and platelet factor 4 on human neutrophils

A Walz, B Dewald, V von Tscharner and M Baggiolini
Theodor-Kocher-Institut, University of Bern, Switzerland.

Platelet basic protein (PBP), connective tissue-activating peptide III (CTAP-III), and platelet factor 4 (PF-4) were purified from human platelet release supernatants by heparin-Sepharose ion-exchange and reversed-phase HPLC, and their neutrophil-activating effects were compared with those of NAP-2, a peptide of 70 amino acids corresponding to part of the sequence of PBP (1) and with sequence homology to NAF/NAP-1. NAP-2-induced elastase release and a rise in cytosolic free Ca2+ at concentrations between 0.3 and 100 nM, and neutrophil chemotaxis at concentrations between 0.03 and 10 nM. It was half as potent as NAF/NAP-1 in inducing exocytosis but showed the same activity in the other responses. By contrast, only minimal if any effects were obtained with PBP, CTAP-III, and PF-4 up to 100 nM. NAP-2 thus appears to behave like a typical chemotactic receptor agonist. It could be generated from PBP and/or CTAP-III released from activated platelets and lead to the accumulation of neutrophils in platelet aggregates.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Gleissner, C. A., von Hundelshausen, P., Ley, K. (2008). Platelet Chemokines in Vascular Disease. Arterioscler. Thromb. Vasc. Bio. 28: 1920-1927 [Abstract] [Full Text]
Yoshimura, T., Oppenheim, J. J. (2008). Chemerin reveals its chimeric nature. JEM 205: 2187-2190 [Abstract] [Full Text]
Woller, G., Brandt, E., Mittelstadt, J., Rybakowski, C., Petersen, F. (2008). Platelet factor 4/CXCL4-stimulated human monocytes induce apoptosis in endothelial cells by the release of oxygen radicals. J. Leukoc. Biol. 83: 936-945 [Abstract] [Full Text]
Pillai, M. M., Iwata, M., Awaya, N., Graf, L., Torok-Storb, B. (2006). Monocyte-derived CXCL7 peptides in the marrow microenvironment. Blood 107: 3520-3526 [Abstract] [Full Text]
Schiemann, F., Grimm, T. A., Hoch, J., Gross, R., Lindner, B., Petersen, F., Bulfone-Paus, S., Brandt, E. (2006). Mast cells and neutrophils proteolytically activate chemokine precursor CTAP-III and are subject to counterregulation by PF-4 through inhibition of chymase and cathepsin G. Blood 107: 2234-2242 [Abstract] [Full Text]
Schaffner, A., King, C. C., Schaer, D., Guiney, D. G. (2004). Induction and antimicrobial activity of platelet basic protein derivatives in human monocytes. J. Leukoc. Biol. 76: 1010-1018 [Abstract] [Full Text]
Pervushina, O., Scheuerer, B., Reiling, N., Behnke, L., Schroder, Jens.-M., Kasper, B., Brandt, E., Bulfone-Paus, S., Petersen, F. (2004). Platelet Factor 4/CXCL4 Induces Phagocytosis and the Generation of Reactive Oxygen Metabolites in Mononuclear Phagocytes Independently of Gi Protein Activation or Intracellular Calcium Transients. J. Immunol. 173: 2060-2067 [Abstract] [Full Text]
Gouwy, M., Struyf, S., Catusse, J., Proost, P., Van Damme, J. (2004). Synergy between proinflammatory ligands of G protein-coupled receptors in neutrophil activation and migration. J. Leukoc. Biol. 76: 185-194 [Abstract] [Full Text]
El-Gedaily, A., Schoedon, G., Schneemann, M., Schaffner, A. (2004). Constitutive and regulated expression of platelet basic protein in human monocytes. J. Leukoc. Biol. 75: 495-503 [Abstract] [Full Text]
Tang, Y.-Q., Yeaman, M. R., Selsted, M. E. (2002). Antimicrobial Peptides from Human Platelets. Infect. Immun. 70: 6524-6533 [Abstract] [Full Text]
Marti, F., Bertran, E., Llucia, M., Villen, E., Peiro, M., Garcia, J., Rueda, F. (2002). Platelet factor 4 induces human natural killer cells to synthesize and release interleukin-8. J. Leukoc. Biol. 72: 590-597 [Abstract] [Full Text]
Schenk, B. I., Petersen, F., Flad, H.-D., Brandt, E. (2002). Platelet-Derived Chemokines CXC Chemokine Ligand (CXCL)7, Connective Tissue-Activating Peptide III, and CXCL4 Differentially Affect and Cross-Regulate Neutrophil Adhesion and Transendothelial Migration. J. Immunol. 169: 2602-2610 [Abstract] [Full Text]
Malawista, S. E., Van Damme, J., Smallwood, J. I., de Boisfleury Chevance, A. (2002). Chemotactic activity of human blood leukocytes in plasma treated with EDTA: chemoattraction of neutrophils about monocytes is mediated by the generation of NAP-2. J. Leukoc. Biol. 72: 175-182 [Abstract] [Full Text]
Gouwy, M., Struyf, S., Mahieu, F., Put, W., Proost, P., Van Damme, J. (2002). The Unique Property of the CC Chemokine Regakine-1 to Synergize with Other Plasma-Derived Inflammatory Mediators in Neutrophil Chemotaxis Does Not Reside in Its NH2-Terminal Structure. Mol. Pharmacol. 62: 173-180 [Abstract] [Full Text]
Struyf, S., Proost, P., Lenaerts, J.-P., Stoops, G., Wuyts, A., Van Damme, J. (2001). Identification of a blood-derived chemoattractant for neutrophils and lymphocytes as a novel CC chemokine, Regakine-1. Blood 97: 2197-2204 [Abstract] [Full Text]
Petersen, F., Bock, L., Flad, H.-D., Brandt, E. (1999). Platelet Factor 4-Induced Neutrophil-Endothelial Cell Interaction: Involvement of Mechanisms and Functional Consequences Different From Those Elicited by Interleukin-8. Blood 94: 4020-4028 [Abstract] [Full Text]
Wuyts, A., D'Haese, A., Cremers, V., Menten, P., Lenaerts, J.-P., De Loof, A., Heremans, H., Proost, P., Van Damme, J. (1999). NH2- and COOH-Terminal Truncations of Murine Granulocyte Chemotactic Protein-2 Augment the In Vitro and In Vivo Neutrophil Chemotactic Potency. J. Immunol. 163: 6155-6163 [Abstract] [Full Text]
Macphee, C. H., Appelbaum, E. R., Johanson, K., Moores, K. E., Imburgia, C. S., Fornwald, J., Berkhout, T., Brawner, M., Groot, P. H. E., O'Donnell, K., O'Shannessy, D., Scott, G., White, J. R. (1998). Identification of a Truncated Form of the CC Chemokine CK{beta}-8 Demonstrating Greatly Enhanced Biological Activity. J. Immunol. 161: 6273-6279 [Abstract] [Full Text]
Petersen, F., Bock, L., Flad, H.-D., Brandt, E. (1998). A Chondroitin Sulfate Proteoglycan on Human Neutrophils Specifically Binds Platelet Factor 4 and Is Involved in Cell Activation. J. Immunol. 161: 4347-4355 [Abstract] [Full Text]
Proost, P., Struyf, S., Couvreur, M., Lenaerts, J.-P., Conings, R., Menten, P., Verhaert, P., Wuyts, A., Damme, J. V. (1998). Posttranslational Modifications Affect the Activity of the Human Monocyte Chemotactic Proteins MCP-1 and MCP-2: Identification of MCP-2(6-76) as a Natural Chemokine Inhibitor. J. Immunol. 160: 4034-4041 [Abstract] [Full Text]
Luster, A. D. (1998). Chemokines -- Chemotactic Cytokines That Mediate Inflammation. NEJM 338: 436-445 [Full Text]
Ludwig, A., Petersen, F., Zahn, S., Gotze, O., Schroder, J.-M., Flad, H.-D., Brandt, E. (1997). The CXC-Chemokine Neutrophil-Activating Peptide-2 Induces Two Distinct Optima of Neutrophil Chemotaxis by Differential Interaction With Interleukin-8 Receptors CXCR-1 and CXCR-2. Blood 90: 4588-4597 [Abstract] [Full Text]
Rollins, B. J. (1997). Chemokines. Blood 90: 909-928 [Full Text]
Jones, S. A., Dewald, B., Clark-Lewis, I., Baggiolini, M. (1997). Chemokine Antagonists That Discriminate between Interleukin-8 Receptors. SELECTIVE BLOCKERS OF CXCR2. J. Biol. Chem. 272: 16166-16169 [Abstract] [Full Text]
Daly, T. J., LaRosa, G. J., Dolich, S., Maione, T. E., Cooper, S., Broxmeyer, H. E. (1995). High Activity Suppression of Myeloid Progenitor Proliferation by Chimeric Mutants of Interleukin 8 and Platelet Factor 4. J. Biol. Chem. 270: 23282-23292 [Abstract] [Full Text]
Bernabei, A., Maione, T. E, Kowalska, M. A., Yan, Z., Niewiarowski, S., Edmunds, L. H. Jr. (1995). Reversal of heparin anticoagulation by recombinant platelet factor 4 and protamine sulfate in baboons during cardiopulmonary bypass. J. Thorac. Cardiovasc. Surg. 109: 765-771 [Abstract] [Full Text]
Malkowski, M. G., Wu, J. Y., Johnson, P. H., Edwards, B. F. P. (1995). The Crystal Structure of Recombinant Human Neutrophil-activating Peptide-2 (M6L) at 1.9-� Resolution. J. Biol. Chem. 270: 7077-7087 [Abstract] [Full Text]
Ehlert, J. E., Petersen, F., Kubbutat, M. H. G., Gerdes, J., Flad, H.-D., Brandt, E. (1995). Limited and Defined Truncation at the C Terminus Enhances Receptor Binding and Degranulation Activity of the Neutrophil-activating Peptide 2 (NAP-2). J. Biol. Chem. 270: 6338-6344 [Abstract] [Full Text]
Groves, D.T., Jiang, Y. (1995). Chemokines, a Family of Chemotactic Cytokines. fake Crit Rev Oral Biol Med 6: 109-118 [Abstract] [Full Text]
Murphy, P., Tiffany, H. (1991). Cloning of complementary DNA encoding a functional human interleukin-8 receptor. Science 253: 1280-1283 [Abstract]
Stoeckelhuber, M., Dobner, P., Baumgartner, P., Ehlert, J., Brandt, E., Mentele, R., Adam, D., Engelmann, B. (2000). Stimulation of Cellular Sphingomyelin Import by the Chemokine Connective Tissue-activating Peptide III. J. Biol. Chem. 275: 37365-37372 [Abstract] [Full Text]