Lymphokine-mediated regulation of the proliferative response of clones of T helper 1 and T helper 2 cells

doi:10.1084/jem.168.2.543

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Lymphokine-mediated regulation of the proliferative response of clones of T helper 1 and T helper 2 cells

R Fernandez-Botran, VM Sanders, TR Mosmann and ES Vitetta
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.

Murine Th1 and Th2 subsets differ not only in the lymphokines they produce, but also functionally. It is not clear what factors influence the preferential activation of one subset versus the other and what regulatory interactions exist between them. The purpose of this study was to examine the effect of lymphokines produced by clones of Th1 cells (IL-2 and IFN-gamma), Th2 cells (IL-4), and APC (IL-1) on the proliferative response of Th1 and Th2 cells after antigenic stimulation. Activation of both types of clones in the presence of antigen and APC resulted in the acquisition of responsiveness to the proliferative effects of both IL-2 and IL-4, although Th2 cells were more responsive to IL-4 than Th1 cells. Responsiveness of Th1 and Th2 cells to both lymphokines decreased with time after initial antigenic activation; Th1 cells lost their responsiveness to IL-4 more rapidly and to IL-2 more slowly than Th2 cells. IFN-gamma partially inhibited the IL-2 and IL-4-mediated proliferation of Th2, but not Th1 cells. Although the presence of IL-1 was not required for the response of Th1 or Th2 cells to IL-4, its presence resulted in a synergistic effect with IL-2 or IL-4 in Th2 but not in Th1 cells. Both subsets responded to a mixture of IL-2 and IL-4 in synergistic fashion. Delayed addition and wash-out experiments indicated that both IL-2 and IL-4 had to be present simultaneously in order for synergy to occur. These results suggest that Th cell subsets might regulate each other via the lymphokines that they secrete and that the pathways of IL-2 and IL-4 mediated proliferation are interrelated.
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Dean, G S, Anand, A, Blofeld, A, Isenberg, D A, Lydyard, P M (2002). Characterization of CD3+CD4-CD8- (double negative) T cells in patients with systemic lupus erythematosus: production of IL-4. Lupus 11: 501-507 [Abstract]
Doyle, A. M., Mullen, A. C., Villarino, A. V., Hutchins, A. S., High, F. A., Lee, H. W., Thompson, C. B., Reiner, S. L. (2001). Induction of Cytotoxic T Lymphocyte Antigen 4 (CTLA-4) Restricts Clonal Expansion of Helper T Cells. JEM 194: 893-902 [Abstract] [Full Text]
Bhattacharyya, S., Ghosh, S., Jhonson, P. L., Bhattacharya, S. K., Majumdar, S. (2001). Immunomodulatory Role of Interleukin-10 in Visceral Leishmaniasis: Defective Activation of Protein Kinase C-Mediated Signal Transduction Events. Infect. Immun. 69: 1499-1507 [Abstract] [Full Text]
Huby, R. D. J., Dearman, R. J., Kimber, I. (2000). Why Are Some Proteins Allergens?. Toxicol Sci 55: 235-246 [Abstract] [Full Text]
Cohn, L., Homer, R. J., Niu, N., Bottomly, K. (1999). T Helper 1 Cells and Interferon {gamma} Regulate Allergic Airway Inflammation and Mucus Production. JEM 190: 1309-1318 [Abstract] [Full Text]
Bellone, G., Turletti, A., Artusio, E., Mareschi, K., Carbone, A., Tibaudi, D., Robecchi, A., Emanuelli, G., Rodeck, U. (1999). Tumor-Associated Transforming Growth Factor-{beta} and Interleukin-10 Contribute to a Systemic Th2 Immune Phenotype in Pancreatic Carcinoma Patients. Am. J. Pathol. 155: 537-547 [Abstract] [Full Text]
Wang, M., Qin, X., Mudgett, J. S., Ferguson, T. A., Senior, R. M., Welgus, H. G. (1999). Matrix metalloproteinase deficiencies affect contact hypersensitivity: Stromelysin-1 deficiency prevents the response and gelatinase B deficiency prolongs the response. Proc. Natl. Acad. Sci. USA 96: 6885-6889 [Abstract] [Full Text]
Boland, S., Baeza-Squiban, A., Fournier, T., Houcine, O., Gendron, M.-C., Chevrier, M., Jouvenot, G., Coste, A., Aubier, M., Marano, F. (1999). Diesel exhaust particles are taken up by human airway epithelial cells in vitro and alter cytokine production. Am. J. Physiol. Lung Cell. Mol. Physiol. 276: L604-L613 [Abstract] [Full Text]
Oriss, T. B., McCarthy, S. A., Campana, M. A. K., Morel, P. A. (1999). Evidence of Positive Cross-Regulation on Th1 by Th2 and Antigen-Presenting Cells: Effects on Th1 Induced by IL-4 and IL-12. J. Immunol. 162: 1999-2007 [Abstract] [Full Text]
Bushell, A., Niimi, M., Morris, P. J., Wood, K. J. (1999). Evidence for Immune Regulation in the Induction of Transplantation Tolerance: A Conditional but Limited Role for IL-4. J. Immunol. 162: 1359-1366 [Abstract] [Full Text]
Hung, K., Hayashi, R., Lafond-Walker, A., Lowenstein, C., Pardoll, D., Levitsky, H. (1998). The Central Role of CD4+ T Cells in the Antitumor Immune Response. JEM 188: 2357-2368 [Abstract] [Full Text]
MISHIMA, H., HOJO, M., WATANABE, A., HAMID, Q.�A., MARTIN, J.�G. (1998). CD4+ T Cells Can Induce Airway Hyperresponsiveness to Allergen Challenge in the Brown Norway Rat. Am. J. Respir. Crit. Care Med. 158: 1863-1870 [Abstract] [Full Text]
Collins, H. L., Schaible, U. E., Kaufmann, S. H. E. (1998). Early IL-4 Induction in Bone Marrow Lymphoid Precursor Cells by Mycobacterial Lipoarabinomannan. J. Immunol. 161: 5546-5554 [Abstract] [Full Text]
Wang, Y., Li, S. P., Moser, S. A., Bost, K. L., Domer, J. E. (1998). Cytokine Involvement in Immunomodulatory Activity Affected by Candida albicans Mannan. Infect. Immun. 66: 1384-1391 [Abstract] [Full Text]
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Salgame, P, Abrams, J., Clayberger, C, Goldstein, H, Convit, J, Modlin, R., Bloom, B. (1991). Differing lymphokine profiles of functional subsets of human CD4 and CD8 T cell clones. Science 254: 279-282 [Abstract]
Moore, K., Vieira, P, Fiorentino, D., Trounstine, M., Khan, T., Mosmann, T. (1990). Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI. Science 248: 1230-1234 [Abstract]
Fernandez-Botran, R., Sanders, V.M., Vitetta, E.S. (1989). Regulation of the Biological Effects of IL-4 on Murine T and B Cells. Cold Spring Harb Symp Quant Biol 54: 705-711 [Abstract]