Journal of Experimental Medicine, Vol 162, 1044-1059, Copyright © 1985 by Rockefeller University Press
Identification of Igh-C-linked determinants on suppressor T cell hybrids and factors specific for L-glutamic acid60-L-alanine30-L- tyrosine10 (GAT)
CM Sorensen, RJ Hayashi and CW Pierce
Hyperimmunization of BALB/c mice with concanavalin A-stimulated blasts from
the Ig allotype-congenic strain, C.B20, results in the production of
antibodies reactive with T cells in an allotype-restricted manner. Spleen
cells from these hyperimmune BALB/c mice were used to generate a panel of
hybridomas that secrete monoclonal antibodies, reactive, in an
allotype-restricted manner, exclusively with T cells subpopulations, and in
particular, reactive with suppressor T cell hybridomas and their secreted
soluble factors. Two functional classes of antibodies were identified:
those that react with single polypeptide-chain suppressor T cell factors
(TsF1) and the suppressor T cell hybridomas that produce such factors, and
those that react with two polypeptide-chain suppressor T cell factors
(TsF2) and their corresponding suppressor T cell hybridomas. These two
classes of antibody were used to isolate molecules from the membranes of
the respective suppressor T cell hybrids that are functionally and
structurally related to the secreted suppressor T cell factors, suggesting
a receptor function for these molecules.
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