T cell-activating properties of an anti-Thy-1 monoclonal antibody. Possible analogy to OKT3/Leu-4

doi:10.1084/jem.159.3.716

This Article

	Full Text (PDF, 1000K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Gunter, K. C.
	Articles by Shevach, E. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Gunter, K. C.
	Articles by Shevach, E. M.


Social Bookmarking

	What's this?

ARTICLES

T cell-activating properties of an anti-Thy-1 monoclonal antibody. Possible analogy to OKT3/Leu-4

KC Gunter, TR Malek and EM Shevach

We have identified a single rat monoclonal antibody, G7, that is a potent inducer of interleukin (IL-2) production from all functioning T cell hybridomas as well as from normal T cells. G7 is also mitogenic for normal T cells and is a very effective inducer of IL-2 receptor expression. On fluorescence-activated cell sorter analysis, G7 recognized a pan-T cell antigen. Immunoprecipitation studies demonstrated that G7 recognized a cell surface molecule of 28-32 kD that appeared to be identical to Thy-1 in coprecipitation studies. In addition, G7 precipitated a protein of 50 kD. The possible relationship of the putative molecular complex identified by G7 on murine cells to the molecular complex identified on human T cells with anti-T3 reagents is discussed. In addition, G7 should prove to be a very useful reagent for studying the early events of lymphocyte activation as well as an inducer of lymphokine-rich supernatants.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Davis, A. H., Guseva, N. V., Ball, B. L., Heusel, J. W. (2008). Characterization of Murine Cytomegalovirus m157 from Infected Cells and Identification of Critical Residues Mediating Recognition by the NK Cell Receptor Ly49H. J. Immunol. 181: 265-275 [Abstract] [Full Text]
Tripathy, S. K., Smith, H. R. C., Holroyd, E. A., Pingel, J. T., Yokoyama, W. M. (2006). Expression of m157, a Murine Cytomegalovirus-Encoded Putative Major Histocompatibility Class I (MHC-I)-Like Protein, Is Independent of Viral Regulation of Host MHC-I. J. Virol. 80: 545-550 [Abstract] [Full Text]
Baudler, S., Baumgartl, J., Hampel, B., Buch, T., Waisman, A., Snapper, C. M., Krone, W., Bruning, J. C. (2005). Insulin-Like Growth Factor-1 Controls Type 2 T Cell-Independent B Cell Response. J. Immunol. 174: 5516-5525 [Abstract] [Full Text]
Haeryfar, S. M. M., Hoskin, D. W. (2004). Thy-1: More than a Mouse Pan-T Cell Marker. J. Immunol. 173: 3581-3588 [Abstract] [Full Text]
Shiratsuchi, A., Watanabe, I., Takeuchi, O., Akira, S., Nakanishi, Y. (2004). Inhibitory Effect of Toll-Like Receptor 4 on Fusion between Phagosomes and Endosomes/Lysosomes in Macrophages. J. Immunol. 172: 2039-2047 [Abstract] [Full Text]
Haeryfar, S. M. M., Al-alwan, M. M., Mader, J. S., Rowden, G., West, K. A., Hoskin, D. W. (2003). Thy-1 Signaling in the Context of Costimulation Provided by Dendritic Cells Provides Signal 1 for T Cell Proliferation and Cytotoxic Effector Molecule Expression, but Fails to Trigger Delivery of the Lethal Hit. J. Immunol. 171: 69-77 [Abstract] [Full Text]
Nikolic, B., Cooke, D. T., Zhao, G., Sykes, M. (2001). Both {{gamma}}{{delta}} T Cells and NK Cells Inhibit the Engraftment of Xenogeneic Rat Bone Marrow Cells and the Induction of Xenograft Tolerance in Mice. J. Immunol. 166: 1398-1404 [Abstract] [Full Text]
Cerasoli, D. M., Kelsoe, G., Sarzotti, M. (2001). CD4+Thy1- thymocytes with a Th-type 2 cytokine response. Int Immunol 13: 75-83 [Abstract] [Full Text]
Hederer, R. A., Guntermann, C., Miller, N., Nagy, P., Szollosi, J., Damjanovich, S., Hale, G., Alexander, D. R. (2000). The CD45 tyrosine phosphatase regulates Campath-1H (CD52)-induced TCR-dependent signal transduction in human T cells. Int Immunol 12: 505-516 [Abstract] [Full Text]
Hong, Y., Maeda, Y., Watanabe, R., Ohishi, K., Mishkind, M., Riezman, H., Kinoshita, T. (1999). Pig-n, a Mammalian Homologue of Yeast Mcd4p, Is Involved in Transferring Phosphoethanolamine to the First Mannose of the Glycosylphosphatidylinositol. J. Biol. Chem. 274: 35099-35106 [Abstract] [Full Text]
Gui, M., Wiest, D. L., Li, J., Kappes, D., Hardy, R. R., Hayakawa, K. (1999). Peripheral CD4+ T Cell Maturation Recognized by Increased Expression of Thy-1/CD90 Bearing the 6C10 Carbohydrate Epitope. J. Immunol. 163: 4796-4804 [Abstract] [Full Text]
Marmor, M. D., Bachmann, M. F., Ohashi, P. S., Malek, T. R., Julius, M. (1999). Immobilization of glycosylphosphatidylinositol-anchored proteins inhibits T cell growth but not function. Int Immunol 11: 1381-1393 [Abstract] [Full Text]
Kukulansky, T., Abramovitch, S., Hollander, N. (1999). Cleavage of the Glycosylphosphatidylinositol Anchor Affects the Reactivity of Thy-1 with Antibodies. J. Immunol. 162: 5993-5997 [Abstract] [Full Text]
Wajeman-Chao, S. A., Lancaster, S. A., Graf, L. H. Jr., Chambers, D. A. (1998). Mechanism of Catecholamine-Mediated Destabilization of Messenger RNA Encoding Thy-1 Protein in T-Lineage Cells. J. Immunol. 161: 4825-4833 [Abstract] [Full Text]
Avni, O., Pur, Z., Yefenof, E., Baniyash, M. (1998). Complement Receptor 3 of Macrophages Is Associated with Galectin-1-Like Protein. J. Immunol. 160: 6151-6158 [Abstract] [Full Text]
Kosugi, A., Saitoh, S.-i., Noda, S., Miyake, K., Yamashita, Y., Kimoto, M., Ogata, M., Hamaoka, T. (1998). Physical and Functional Association between Thymic Shared Antigen-1/Stem Cell Antigen-2 and the T Cell Receptor Complex. J. Biol. Chem. 273: 12301-12306 [Abstract] [Full Text]
Gobel, T. W. F., Bolliger, L. (1998). Cutting Edge: The Chicken TCR {zeta}-Chain Restores the Function of a Mouse T Cell Hybridoma. J. Immunol. 160: 1552-1554 [Abstract] [Full Text]
Diep, D. B., Nelson, K. L., Raja, S. M., Pleshak, E. N., Buckley, J. T. (1998). Glycosylphosphatidylinositol Anchors of Membrane Glycoproteins Are Binding Determinants for the Channel-forming Toxin Aerolysin. J. Biol. Chem. 273: 2355-2360 [Abstract] [Full Text]
Hide, M., Francis, D. M., Grattan, C., Hakimi, J., Kochan, J. P., Greaves, M. W. (1993). Autoantibodies against the High-Affinity IgE Receptor as a Cause of Histamine Release in Chronic Urticaria. NEJM 328: 1599-1604 [Abstract] [Full Text]
Takahama, Y, Singer, A (1992). Post-transcriptional regulation of early T cell development by T cell receptor signals. Science 258: 1456-1462 [Abstract]
Letourneur, F, Klausner, R. (1992). Activation of T cells by a tyrosine kinase activation domain in the cytoplasmic tail of CD3 epsilon. Science 255: 79-82 [Abstract]
Frank, S., Niklinska, B., Orloff, D., Mercep, M, Ashwell, J., Klausner, R. (1990). Structural mutations of the T cell receptor zeta chain and its role in T cell activation. Science 249: 174-177 [Abstract]
Lefrancois, L, Goodman, T (1989). In vivo modulation of cytolytic activity and Thy-1 expression in TCR-gamma delta+ intraepithelial lymphocytes. Science 243: 1716-1718 [Abstract]
Shih, C., Truitt, R. (1987). Downregulation of L3T4+ cytotoxic T lymphocytes by interleukin-2. Science 238: 344-347 [Abstract]
Tse, A., Barclay, A., Watts, A, Williams, A. (1985). A glycophospholipid tail at the carboxyl terminus of the Thy-1 glycoprotein of neurons and thymocytes. Science 230: 1003-1008 [Abstract]
Hong, Y., Maeda, Y., Watanabe, R., Inoue, N., Ohishi, K., Kinoshita, T. (2000). Requirement of PIG-F and PIG-O for Transferring Phosphoethanolamine to the Third Mannose in Glycosylphosphatidylinositol. J. Biol. Chem. 275: 20911-20919 [Abstract] [Full Text]