Activation of murine B lymphocytes by anti-immunoglobulin is an inductive signal leading to immunoglobulin secretion

doi:10.1084/jem.152.1.138

This Article

	Full Text (PDF)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Parker, D. C.
	Articles by Schneider, G. B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Parker, D. C.
	Articles by Schneider, G. B.


Social Bookmarking

	What's this?

ARTICLES

Activation of murine B lymphocytes by anti-immunoglobulin is an inductive signal leading to immunoglobulin secretion

DC Parker, DC Wadsworth and GB Schneider

Cultures of isolated mouse splenic B lymphocytes activated by the divalent F(ab')2 fragment of purified rabbit anti-mouse Fab or class- specific anti-mouse IgM antibodies can be driven on to high rate Ig secretion by the addition of the supernatant fluid of a 24-h culture of concanavalin A-activated spleen cells (SN). The polyclonal antibody response to anti-Ig pus SN is comparable in magnitude with the lipopolysaccharide response as measured in a reverse plaque assay. The addition of SN can be delayed for 24 h after addition of anti-Ig without changing the kinetics of the response. Addition at 48 h delays the response by 24 h. The response to F(ab')2 anti-Fab plus SN is sensitive to Fc-dependent inhibition because intact anti-Fab antibodies inhibit strongly at relatively low concentrations. The monovalent Fab' fragment fails to induce Ig secretion, indicating that cross-linkage of surface immunoglobulin is required. Although the production of active SN is T cell dependent, the response to anti-Ig plus SN is T independent. These findings are interpreted as a polyclonal model of a thymus-dependent antibody response. X
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Kanswal, S., Katsenelson, N., Selvapandiyan, A., Bram, R. J., Akkoyunlu, M. (2008). Deficient TACI Expression on B Lymphocytes of Newborn Mice Leads to Defective Ig Secretion in Response to BAFF or APRIL. J. Immunol. 181: 976-990 [Abstract] [Full Text]
Skalet, A. H., Isler, J. A., King, L. B., Harding, H. P., Ron, D., Monroe, J. G. (2005). Rapid B Cell Receptor-induced Unfolded Protein Response in Nonsecretory B Cells Correlates with Pro- Versus Antiapoptotic Cell Fate. J. Biol. Chem. 280: 39762-39771 [Abstract] [Full Text]
Ait-Azzouzene, D., Verkoczy, L., Peters, J., Gavin, A., Skog, P., Vela, J. L., Nemazee, D. (2005). An immunoglobulin C{kappa}-reactive single chain antibody fusion protein induces tolerance through receptor editing in a normal polyclonal immune system. JEM 201: 817-828 [Abstract] [Full Text]
Zuniga, E., Rabinovich, G. A., Iglesias, M. M., Gruppi, A. (2001). Regulated expression of galectin-1 during B-cell activation and implications for T-cell apoptosis. J. Leukoc. Biol. 70: 73-79 [Abstract] [Full Text]
Nosanchuk, J. D., Rosas, A. L., Casadevall, A. (1998). The Antibody Response to Fungal Melanin in Mice. J. Immunol. 160: 6026-6031 [Abstract] [Full Text]