Biosynthesis of the complement components and the regulatory proteins of the alternative complement pathway by human peripheral blood monocytes

doi:10.1084/jem.151.3.501

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Biosynthesis of the complement components and the regulatory proteins of the alternative complement pathway by human peripheral blood monocytes

K Whaley

Short-term cultures of human peripheral blood monocytes were shown to synthesize the alternative pathway complement components C3, factors B (B) and D (D), and properdin, the regulatory proteins C3b inactivator (C3bINA) and beta 1H, in addition to C2, C4, and C5. B, D, properdin, C3bINA, and C2 were detected by functional assays, whereas beta 1H, C4, C3, and C5 could only be detected using immunochemical procedures. Immunoperoxidase localization studies showed that all the cells in each culture contained each component, so it is possible that all monocytes synthesize each component. It is concluded that cells of the monocyte- macrophage series form a mobile source of complement components and regulatory proteins which can be concentrated at sites of inflammation.
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Mevorach, D., Mascarenhas, J. O., Gershov, D., Elkon, K. B. (1998). Complement-dependent Clearance of Apoptotic Cells by Human Macrophages. JEM 188: 2313-2320 [Abstract] [Full Text]
Fischer, M. B., Ma, M., Hsu, N. C., Carroll, M. C. (1998). Local Synthesis of C3 Within the Splenic Lymphoid Compartment Can Reconstitute the Impaired Immune Response in C3-Deficient Mice. J. Immunol. 160: 2619-2625 [Abstract] [Full Text]
Taylor, P. R., Nash, J. T., Theodoridis, E., Bygrave, A. E., Walport, M. J., Botto, M. (1998). A Targeted Disruption of the Murine Complement Factor B Gene Resulting in Loss of Expression of Three Genes in Close Proximity, Factor B, C2, and D17H6S45. J. Biol. Chem. 273: 1699-1704 [Abstract] [Full Text]
Ault, B. H., Schmidt, B. Z., Fowler, N. L., Kashtan, C. E., Ahmed, A. E., Vogt, B. A., Colten, H. R. (1997). Human Factor H Deficiency. MUTATIONS IN FRAMEWORK CYSTEINE RESIDUES AND BLOCK IN H PROTEIN SECRETION AND INTRACELLULAR CATABOLISM. J. Biol. Chem. 272: 25168-25175 [Abstract] [Full Text]
Akama, H., Johnson, C. A. C., Colten, H. R. (1995). Human Complement Protein C2. J. Biol. Chem. 270: 2674-2678 [Abstract] [Full Text]
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