Journal of Experimental Medicine, Vol 147, 13-24, Copyright © 1978 by Rockefeller University Press
Migratory behavior of lymphocytes with specific reactivity to alloantigens. II. Selective recruitment to lymphoid cell allografts and their draining lymph nodes
EE Emeson
A dual-antigen, dual-isotope assay has been used to monitor the migratory
behavior of selectively labeled antiallogeneic lymphocytes in mice
challenged subcutaneously in all four foot pads with semiallogeneic spleen
cells. 3H-labeled anti-C3H and 14C-labeled anti- C57BL lymphocytes of
DBA/2J origin were pooled and adoptively transferred to multiple groups of
previously challenged DBA/2J recipients. In some of the studies, separate
groups of recipients were challenged with either CDF or BDF spleen cells in
all four paws, whereas in others CDF spleen cells were used to challenge
the right paws of each mouse in the group and BDF spleen cells to challenge
the left paws of each mouse in the group. At intervals varying from 24 to
96 h after challenge, a subgroup of four mice from each appropriate group
was sacrificed and the relative numbers of anti-C3H and anti- C57BL
lymphocytes present in the challenged paws, draining lymph nodes, and other
tissues of each mouse were inferred from the mean 3H/14C ratios of the
respective tissues of that subgroup. The results of these studies firmly
establish that specific antiallogeneic lymphocytes are selectively
recruited to the paws and draining lymph nodes of mice challenged
subcutaneously in the foot pads with semiallogeneic spleen cells and are
deleted from their circulating blood and nondraining lymph nodes. A
mechanism for antigen-induced selective recruitment and its possible
functional significance in tumor immunology are discussed.
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