The Journal of Experimental Medicine, Vol 127, 43-53,
Copyright © 1968 by The Rockefeller University Press
HAPTEN CARRIER RELATIONSHIPS IN THE DNP-PLL·FOREIGN ALBUMIN COMPLEX SYSTEM: INDUCTION OF TOLERANCE AND STIMULATION OF CELLS IN VITRO
Ira Green M.D.1,
William E. Paul M.D.1, and
Baruj Benacerraf M.D.1
1 From the Department of Pathology, New York University School of Medicine, New York 10016
Genetic nonresponder guinea pigs made tolerant to BSA and then immunized with DNP-PLL·BSA failed to make anti-DNP-PLL antibodies. Thus, tolerance to a carrier protein renders animals unresponsive to the hapten which it bears.
The addition in vitro of DNP-PLL or DNP-GL to lymph node cell cultures derived from genetic responder animals immunized with these materials led to a significant stimulation of 3H-thymidine incorporation into DNA. However, the addition of DNP-PLL or DNP-GL to lymph node cell cultures from nonresponder animals immunized with these materials failed to produce any stimulation of DNA synthesis. Furthermore, the addition of DNP-PLL to lymph node cell cultures from nonresponder animals immunized with DNP-PLL·BSA or DNP-PLL·OVA also failed to stimulate cell proliferation in spite of the fact that the lymph node cells of these animals were producing anti-DNP-PLL antibodies.
The above facts suggest that the function of the PLL gene product is to act at an early crucial step in the immune mechanism to form an antigen-inducer complex. The specificity of this early step may be of a simple order and different than that of the antibody which is later produced in the immune response.
Submitted on August 7, 1967
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