The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 127, 109-118, Copyright © 1968 by The Rockefeller University Press

ARTICLE

ULTRASTRUCTURAL LOCALIZATION OF ANTIBODY IN DIFFERENTIATING PLASMA CELLS

Elizabeth H. Leduc Ph.D.1, Stratis Avrameas Ph.D.1, and Michel Bouteille M.D.1

1 From the Division of Biological and Medical Sciences, Brown University, Providence, Rhode Island 02912, and the Laboratoires de Chimie des Protéines et de Microscopic Electronique, Institut de Recherches sur le Cancer, Villejuif, France

Antibody was localized by electron microscopy within differentiating and mature plasma cells of the spleens of hyperimmunized rabbits. Horseradish peroxidase was used as antigen. Intracellular antibody to peroxidase was revealed in glutaraldehyde-fixed tissue by coupling it with its antigen and then revealing the sites of peroxidase activity cytochemically. Antibody first appears in the perinuclear space of hemocytoblasts where it persists through differentiation into immature plasma cells, but it disappears from this site in mature plasma cells. Concomitant with the development of the ergastoplasm, antibody accumulates in many but not all of its cisternae. Antibody is present in the lamellar portion of the Golgi apparatus in all phases of plasmacytic differentiation. Mature plasma cells exhibit two types of antibody distribution, a concentration into large spherical intracisternal granules or an overflowing into all parts of the cytoplasm.

Submitted on August 22, 1967


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