The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 118, 175-194, Copyright © 1963, by The Rockefeller Institute

ARTICLE

THE ACTION OF ENZYMES FROM CLOSTRIDIUM TERTIUM ON THE I ANTIGENIC DETERMINANT OF HUMAN ERYTHROCYTES

Donald M. Marcus M.D.1, Elvin A. Kabat Ph.D.1, and Richard E. Rosenfield M.D.1

1 From the Departments of Microbiology and Neurology, College of Physicians and Surgeons, Columbia University and the Neurological Institute, Presbyterian Hospital, and the Department of Hematology, the Mount Sinai Hospital, New York

A method was described for the partial purification of beta galactosidase and beta glucosaminidase from Clostridium tertium culture supernatants. Treatment of erythrocytes with preparations containing both enzymes decreases their ability to react with anti-I cold agglutinins, and with Type XIV antipneumococcal horse serum. Erythrocytes of blood group A1 are altered more rapidly and extensively than are group O cells. The enzymatic treatment of stroma results in a decrease in ability to absorb anti-I agglutinins and the release of galactose and N-acetylglucosamine as monosaccharides. The data suggest that these two sugars may be structural units of the erythrocyte I determinant, but no direct evidence is available.

Submitted on March 7, 1963


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