The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 117, 603-620, Copyright ©, 1963, by The Rockefeller Institute

ARTICLE

THE ASSOCIATION OF SKIN-SENSITIZING ANTIBODY WITH THE ß2A-GLOBULINS IN SERA FROM RAGWEED-SENSITIVE PATIENTS

Philip Fireman M.D.1, Wilton E. Vannier M.D.1, and Howard C. Goodman M.D.1

1 From the Laboratory of Clinical Investigations and the Laboratory of Immunology, the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda

1. The removal of the ß2A-globulins from three sera from treated ragweed-sensitive individuals by immune absorption was associated with the loss of all detectable skin-sensitizing antibody activity as demonstrated by Prausnitz-Küstner testing.

2. Gel filtration studies, with sephadex G-200, indicated that the fractions containing only macroglobulins were devoid of all detectable skin-sensitizing antibody activity.

3. The immune absorption of the gamma-globulins from a serum fraction containing ß2A-globulins, gamma-globulins, and a trace of ß2M-globulins had no detectable influence on the skin-sensitizing antibody activity.

4. The removal of a portion of the albumin from the allergic sera by immune absorption, with retention of the skin-sensitizing activity, indicated that the loss of skin-sensitizing antibody was not due to non-specific absorption on an antigen-antibody precipitate.

5. No inhibition of the Prausnitz-Küstner reaction was observed when sheep serum, normal human serum, or normal human gamma-globulins were tested in concentrations described.

6. We conclude that the skin-sensitizing antibody activities in the three sera from treated ragweed-sensitive individuals studied were associated with the ß2A-globulins.

 Submitted on December 6, 1962


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