The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 829K)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schwab, J. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schwab, J. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of Experimental Medicine, Vol 116, 17-28, Copyright, 1962, by The Rockefeller Institute

ARTICLE

ANALYSIS OF THE EXPERIMENTAL LESION OF CONNECTIVE TISSUE PRODUCED BY A COMPLEX OF C POLYSACCHARIDE FROM GROUP A STREPTOCOCCI : I. IN VIVO REACTION BETWEEN TISSUE AND TOXIN



John H. Schwab Ph.D.1

1 From the Department of Bacteriology and Immunology, University of North Carolina School of Medicine, Chapel Bill

Specific antibody and a glucosaminidase enzyme react with the serologically active sites on C polysaccharide, and local injection of these reagents will neutralize the toxic effect of C polysaccharide complexes even after an interval of 24 to 72 hours.

The cell wall lysins in S. albus filtrate and from phage-lysed Group C streptococci, break down the cell wall structure of Group A streptococci but leave the serological reactive sites of the C polysaccharide intact. These reagents lose much of their ability to neutralize the C polysaccharide toxin when superinjected after an interval of 4 hours following toxin injections.

Toxic C polysaccharide cannot be recovered from an injection site by intercellular perfusion of the excised skin area after an interval of 1 to 4 hours following toxin injection.

It is concluded that toxic particles of C polysaccharide complexes combine firmly with the dermal tissue of rabbits within 1 to 4 hours following intracutaneous injection. The cell wall lysins neutralize the toxin by reducing the particle size of C polysaccharide complexes. This minimum particle size is required for the initial reaction with tissue and when this has occurred these reagents are no longer able to influence the development of the lesion.

 Submitted on March 4, 1962


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS