The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 115, 1107-1115, Copyright, 1962, by The Rockefeller Institute

ARTICLE

THE CHARACTERIZATION OF STAPHYLOCOCCAL TOXINS : II. THE ISOLATION AND CHARACTERIZATION OF A HOMOGENEOUS STAPHYLOCOCCAL PROTEIN POSSESSING ALPHA HEMOLYTIC, DERMONECROTIC, LETHAL, AND LEUCOCIDAL ACTIVITIES



Surendra Kumar 1, Keith I. Loken D.V.M.1, Alan J. Kenyon D.V. M.1, and Robert K. Lindorfer Ph.D.1

1 From the Division of Veterinary Bacteriology and Public Health, University of Minnesota, St. Paul

The continuous flow paper electrophoretic methods used to isolate alpha hemolysin are presented.

The purity of the alpha hemolysin preparation is demonstrated by double agar diffusion, immunoelectrophoretic, and ultracentrifugation techniques.

Indirect evidence of purity is provided by the fact that rabbit immunized with purified alpha hemolysin produced detectable antibody only to alpha hemolysin.

Direct support is given to the "unitarian theory" by the demonstration that purified alpha hemolysin also possessed dermonecrotic, lethal, and leucocidal activities.

Further studies dependent on the availability of purified alpha hemolysin are outlined.

 Submitted on January 18, 1962


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