The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 115, 763-775, Copyright, 1962, by The Rockefeller Institute

ARTICLE

AN ALUMINUM MARKER FOR THE DIFFERENTIATION AND SEPARATION OF VIRULENT AND ATTENUATED POLIOVIRUSES

Craig Wallis 1, Joseph L. Melnick Ph.D.1, George D. Ferry 1, and Ira L. Wimberly 1

1 From the Department of Virology and Epidemiology, Baylor University College of Medicine, Houston, Texas

A new character, the A marker, for polioviruses is described. In the presence of Al+++ (1 to 100 mM) attenuated, but not virulent, strains of type 1 and type 2 polioviruses are stabilized so that they resist thermal inactivation at 50°C. Differences between the attenuated and virulent type 3 strains studied were of the opposite character and less marked.

By the use of optimal concentrations of Al+++ (12 mM for type 1 and 1 mM for type 2) attenuated strains can be separated from mixtures containing virulent type 1 or 2 strains.

The A marker was found to be correlated with the d but not the rct/40 or T marker. The A marker has been used for following genetic changes of vaccine strains after their multiplication in man.

 Submitted on January 1, 1962


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