The Journal of Experimental Medicine
Fluorescence In Vivo Endomicroscopy
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 785K)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shermer, R. W.
Right arrow Articles by Brinkhous, K. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shermer, R. W.
Right arrow Articles by Brinkhous, K. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of Experimental Medicine, Vol 114, 905-920, Copyright, 1961, by The Rockefeller Institute

ARTICLE

STUDIES ON THROMBIN-INDUCED PLATELET AGGLUTINATION

Richard W. Shermer 1, Reginald G. Mason 1, Robert H. Wagner Ph.D.1, and Kenneth M. Brinkhous M.D.1

1 From the Department of Pathology, School of Medicine, University of North Carolina, Chapel Hill

A one-stage macroscopic test for platelet agglutination was used to study the effect of thrombin and thrombin-cation mixtures on washed platelets. Conclusions regarding platelet agglutination are as follows:

(a) Canine, bovine, or human thrombin alone does not cause agglutination of canine or human platelets.

(b) Thrombin with calcium or magnesium causes rapid platelet agglutination. Both calcium and magnesium are active at physiologic concentrations. Divalent manganese or cadmium ions can be substituted for calcium or magnesium.

(c) The agglutination reaction is affected but little by the species of origin of thrombin or platelets, or by variations in ionic strength or pH over a broad range.

(d) Temperature at which the reaction is carried out is critical; optimal temperature for the test is 28°C.

(e) Agglutination is inhibited by high ionic strength, by pH values outside the range 6.4–8.6, and by temperatures outside the range 25–28°C. High concentrations of calcium have a specific inhibitory effect.

(f) Platelet agglutination time is as sensitive an index of thrombin concentration as is the fibrinogen clotting time.

A comparison is made between divalent cations which influence platelet agglutination induced by thrombin, TAg', and TAg. A similar comparison is made of cations influencing the action of thrombin on the "substrates," fibrinogen, TAMe, and platelets.

 Submitted on July 10, 1961


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS