Councilman Morgan M.D.¹, Konrad C. Hsu Ph.D.¹, Richard A. Rifkind M.D.¹, Alice W. Knox Ph.D.¹, and Harry M. Rose M.D.¹

¹ From the Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York

Freezing of the chorioallantoic membrane after brief fixation in formalin preserves antigenicity of cellular components and allows penetration of ferritin-conjugated antibody. Dense aggregates of viral antigen, presumed to be of the soluble type, were found in the nuclei of cells infected with influenza virus. Intracytoplasmic antigen, on the other hand, was widely dispersed. The experimental observations are consistent with the hypothesis that soluble antigen diffuses into the cytoplasm through intact nuclear membranes. Nucleoli do not appear to be the sites of antigen synthesis.

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• Tani, E., Kawamura, Y., Ametani, T., Handa, H., Imura, H., Kato, Y. (1969). Immunocytochemistry of Acidophil. Arch Neurol 20: 634-643 [Abstract]  
• Morgan, C., Rifkind, R. A., Rose, H. M. (1962). The Use of Ferritin-conjugated Antibodies in Electron Microscopic Studies of Influenza and Vaccinia Viruses. Cold Spring Harb Symp Quant Biol 27: 57-65 [Abstract]  
• Bernhard, W., Tournier, P. (1962). Ultrastructural Cytochemistry Applied to the Study of Virus Infection. Cold Spring Harb Symp Quant Biol 27: 67-82 [Abstract]  

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