The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 110, 341-353, Copyright, 1959, by The Rockefeller Institute

ARTICLE

ANALYSIS OF THE PROTEINS OF RAT SERUM BY STARCH ELECTROPHORESIS AND BY CATIONIC DETERGENT ANALYSIS : I. IDENTIFICATION OF AN UNUSUAL GLOBULIN



Ralph F. Jacox M.D.1

1 From the Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York

A protein of unusual characteristics has been identified in normal rat sera by electrophoretic separation at pH 8.6, followed by precipitation of each fraction with a cationic detergent. This protein, which is closely identified with albumin after electrophoresis at pH 8.6, precipitates with cationic detergent at a low pH in contrast to albumin which precipitates with detergent only at a high pH. This protein has characteristics of a globulin and is designated as a "fast alpha fraction."

This fraction and albumin are present in about equal amounts in sera of normal rats. A separation of the fast alpha fraction can be made by electrophoresis in an acetate buffer of pH 4.25 and by precipitation according to a modified Cohn, cold-alcohol technique.

A protein of similar characteristics has not been found in either human or rabbit sera studied by the same method of electrophoresis and subsequent sub-fractionation with cationic detergent.

 Submitted on April 21, 1959


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