The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 110, 207-219, Copyright, 1959, by The Rockefeller Institute

ARTICLE

THE EFFECT OF ANTIGENIC STIMULATION ON INCORPORATION OF PHOSPHATE AND METHIONINE INTO PROTEINS OF ISOLATED LYMPH NODE CELLS

Milton Kern Ph.D.1 and Herman N. Eisen M.D.1

1 Division of Dermatology, Department of Internal Medicine, Washington University School of Medicine, and the Barnard Free Skin and Cancer Hospital, St. Louis

Isolated lymph node cells incorporate inorganic orthophosphate into a protein fraction. The phosphorylated product is a phosphoprotein. The rate of phosphate incorporation into phosphoprotein was determined in cells isolated from regional lymph nodes at varying times after antigen injection. The rate was unaltered on the 3rd day, but was enhanced on the 4th day after injection. Parallel results were obtained with L-methionine incorporation into the same gross protein fraction. Possible relationships between antibody formation and the observed enhancement in phosphate incorporation into phosphoprotein are discussed.

Submitted on March 31, 1959


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