The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 109, 271-292, Copyright, 1959, by The Rockefeller Institute

ARTICLE

STUDY ON GROWTH OF RICKETTSIAE : V. PENETRATION OF RICKETTSIA TSUTSUGAMUSHI INTO MAMMALIAN CELLS IN VITRO



Zanvil A. Cohn M.D.1, F. Marilyn Bozeman 1, Janis M. Campbell 1, James W. Humphries 1, and Thomas K. Sawyer 1

1 From the Department of Rickettsial Diseases, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C.

A system has been described in which the penetration of Rickettsia tsutsugamushi (Karp strain) into tissue culture cells can be quantitated, and the factors affecting this process studied. The results indicated that rickettsial penetration in vitro depended largely on the viability of the organisms. Certain components of the fluid environment such as the divalent cations and protein were found to be of importance. The temperature dependence of the penetration process was found to vary with the nature of the suspending medium. A number of compounds related to L-glutamic acid enhanced penetration, whereas metabolic inhibitors depressed this process. Aureomycin at concentrations between 50 and 250 µg./ml. inhibited the penetration of rickettsiae while chloramphenicol at similar concentrations was ineffective. The results are discussed in terms of the biological and biochemical properties of this group of agents.

Submitted on November 30, 1958


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