GENETICS OF SOMATIC MAMMALIAN CELLS: III. LONG-TERM CULTIVATION OF EUPLOID CELLS FROM HUMAN AND ANIMAL SUBJECTS

doi:10.1084/jem.108.6.945

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The Journal of Experimental Medicine, Vol 108, 945-956, Copyright, 1958, by The Rockefeller Institute

ARTICLE

GENETICS OF SOMATIC MAMMALIAN CELLS : III. LONG-TERM CULTIVATION OF EUPLOID CELLS FROM HUMAN AND ANIMAL SUBJECTS

Theodore T. Puck Ph.D.¹, Steven J. Cieciura Ph.D.¹, and Arthur Robinson M.D.¹

¹ From the Department of Biophysics, Florence R. Sabin Laboratories, and the Department of Pediatrics, University of Colorado Medical School, Denver

A methodology designed to eliminate mitotic inhibitor actionand involving use of pretested fetal calf serum and carefulpH and temperature control has been described by which cellsfrom normal human and animal tissue can be maintained in activegrowth for long periods in vitro without development of aneuploidy.

Bymeans of this procedure, it is possible reliably to establishcell cultures from minute skin biopsies which can be taken fromany individual.

Clones of mammalian cells with chromosomal markershave been isolated by this means from x-irradiated non-irradiatedcell cultures.

Application of these techniques to chromosomedelineation in large numbers of human subjects; determinationof chromosomal sex in patients; spontaneuos and induced geneticchanges in somatic mammalian cells in vivo and in vitro; comparisonof metabolic differences between normal and cancerous cellsand other problems have been indicated.

Submitted on July 24, 1958

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